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Calf articular cartilage in organ culture in a chemically defined medium

1. Autoradiographic study after [35S]sulfate labeling

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Summary

Articular cartilage from 5-month-old calves survived for 4 weeks in organ culture in a chemically defined medium. The viability was assessed by calculating the labeling frequency of chondrocytes on autoradiograms after [35S]sulfate labeling. The survival rate of chondrocytes was highest in 20% oxygen tension, whereas 50% oxygen resulted in chondrocyte death after 2 weeks. The survival rate was improved by using 1-mm thick standardized pieces of cartilage. The cells of the superficial layer showed a higher proportion of living chondrocytes than the cells in the deeper layers. No differentiation, dedifferentiation, or outgrowth of cells occurred during the experimental period.

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Authors and Affiliations

  1. Departments of Periodontology and Orthopaedic Surgery, University of Ume∢, S-901, 87 Ume∢, Sweden

    Axel Bergenholtz & Rudolf K. Lemperg

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  1. Axel Bergenholtz
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  2. Rudolf K. Lemperg
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This work was supported by a grant from the Swedish Medical Research council project no. 17 X-138.

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Bergenholtz, A., Lemperg, R.K. Calf articular cartilage in organ culture in a chemically defined medium. In Vitro 11, 286–290 (1975). https://doi.org/10.1007/BF02615639

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