References
Morgan, J. F., H. J. Morton, and R. C. Parker. 1950. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. Exp. Biol. Med. 73: 1–8.
Evans, V. J., W. R. Earle, K. K. Sanford, J. E. Shannon, and H. K. Waltz. 1951. The preparation and handling of replicate tissue cultures for quantitative studies. J. Natl. Cancer Inst. 11: 907–927.
Puck, T. T., P. I. Marcus, and S. J. Cieciura. 1956. Clonal growth of mammalian cellsin vitro. Growth characteristics of colonies from single HeLa cells with and without a ‘feeder’ layer. J. Exp. Med. 103: 273–284.
Healy, G. M., and R. C. Parker. 1966. An improved chemically defined basal medium (CMRL-1415) for newly explanted mouse embryo cells. J. Cell Biol. 30: 531–538.
Healy, G. M., S. Teleki, A. V. Seefried, M. J. Walton, and H. G. Macmorine. 1971: Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Appl. Microbiol. 21: 1–5.
Waymouth, C. 1965. Thecultivation of cells in chemically defined medium and the malignant transformation of cellsin vitro. In: C. V. Ramakrishnan (Ed.)Tissue Culture. W. Junk. The Hague, pp. 168–179.
Holley, R. W., and J. A. Kiernan. 1971. Studies of serum factors required by 3T3 and SV 3T3 cells. In: G. E. W. wolstenholme, and J. Knight (Eds.),Growth Control in Cell Cultures. Ciba Foundation Symposium. Churchill Livingstone, Ediburgh, pp. 3–15.
Temin, H. W., R. W. Pierson, Jr., and N. C. Dulak. 1972. The role of serum in the control of multiplication of avian and mammalian cells in culture. In: G. H. Rothblat, and V. J. Cristofalo (Eds.),Growth, Nutrition and Metabolism of Cells in Culture. Vol. 1 Academic Press, New York, pp. 49–81.
Kaighn, M. E., and A. M. Prince. 1971. Production of albumin and other serum proteins by clonal cultures of normal human liver. Proc. Natl. Acad. Sci. U.S.A. 68: 2396–2400.
Ham, R. G. 1963. An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res. 29: 515–526.
Ham, R. G. 1965. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. U.S.A. 53: 288–293.
Coon, H.G. 1966. Clonal stability and phenotypic expression of chick cartilage cellsin vitro. Proc. Natl. Acad. Sci. U.S.A. 55: 66–73.
Coon, H. G., and M. C. Weiss. 1969. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids. Proc. Natl. Acad. Sci. U.S.A. 62: 852–859.
Ham, R. G. 1967. Discussion following talk by C. Waymouth at Second Decennial Review Conference on Cell, Tissue and Organ Culture. Natl. Cancer Inst. Monogr. 26: 19–21.
Freed, J. J., and S. A. Schatz. 1969. Chromosome aberrations in cultured cells deprived of single essential amino acids. Exp. Cell Res. 55: 393–409.
Stanbridge, E. J., L. Hayflick, and F. T. Perkins. 1971. Modification of amino acid concentrations induced by Mycoplasmas in cell culture medium. Nature [New Biol.] 232: 242–244.
Kraemer, P. M. 1964. Interaction of Mycoplasma (PPLO) and murine lymphoma cell cultures: prevention of cell lysis by arginine. Proc. Soc. Exp. Biol. Med. 115: 206–212.
Miller, G., J. Emmons, and D. Stitt. 1971. Susceptibility of human lymphoblastoid cell lines to a cytopathic effect induced by an arginine-utilizing Mycoplasma strain. J. Infect. Dis. 124: 322–326.
Lieberman, I., and P. Ovc. 1960. Inhibition of growth of cultured mammalian cells by liver extracts. Biochim. Biophys. Acta 38: 153.
Holley, R. W. 1967. Evidence that a rat liver “inhibitor” of the synthesis of DNA in cultured mammalian cells is arginase. Biochim. Biophys. Acta 145: 525–527.
Freed, J. J., and S. Sorof. 1967. The nature of the inhibition of replication of cultured cells by a liver macromolecule. Wistar Inst. Symp. Monogr. 7: 15–24.
Sorof, S., E. M. Young, L. Luongo, V. M. Kish, and J. J. Freed. 1967. Inhibition of cell multiplicationin vitro by liver arginase. Wistar Inst. Symp. Monogr. 7: 25–37.
Kihara, H., and S. D. De La Flor. 1968. Arginase in fetal calf serum. Proc. Soc. Exp. Biol. Med. 129: 303–304.
Eagle, H. 1959. Amino acid metabolism in mammalian cell cultures. Science 130: 432–437.
Blaker, G. J., and S. J. Pirt. 1971. The uptake of vitamins by mouse fibroblast cells (strain LS) during growth in a chemically defined medium. J. Cell Sci. 8: 701–708.
Hakala, M. T. 1957. Prevention of toxicity of amethopterin for sarcoma 180 cells in tissue culture. Science 126: 255.
Lieberman, I., and P. Ove. 1960. Control of growth of mammalian cells in culture with folic acid, thymidine and purines. J. Biol. Chem. 235: 1119–1123.
Morris, N. R., and G. A. Fischer. 1960. Studies concerning inhibition of the synthesis of deoxycytidine by phosphorylated derivative of thymidine. Biochim. Biophys. Acta 42: 183–184.
Thomas, J. A., and M. J. Johnson. 1967. Trace metal requirements of NCTC clone 929 strain L cells. J. Natl. Cancer Inst. 39: 337–345.
Ham, R. G. 1964. Putrescine and related amines as growth factors for a mammalian cell line. Biochem. Biophys. Res. Commun. 14: 34–38.
Alarcon, R. A. 1964. Isolation of acrolein from incubated mixtures of spermine with calf serum and its effects on mammalian cells. Arch. Biochem. Biophys. 106: 240–242.
Pohjanpelto, P., and A. Raina. 1972. Identification of a growth factor produced by human fibroblastsin vitro as putrescine. Nature New Biol. 235: 247–249.
Spector, A. A. 1972. Fatty acid, glyceride and phospholipid metabolism. In: G. H. Rothblat, and V. J. Cristofalo (Eds.),Growth, Nutrition, and Metabolism of Cells in Culture. Vol. 1. Academic Press, New York, pp. 257–296.
Bailey, J. M., and L. M. Dunbar. 1973. Essential fatty acid requirements of cells in tissue culture. Exp. Mol. Pathol. 18: 142–161.
Neuman, R. E., and A. A. Tytell. 1960. Serumless medium for cultivation of cells of normal and malignant origin. Proc. Soc. Exp. Biol. Med. 104: 252–256.
Ham, R. G. 1963. Albumin replacement by fatty acids in clonal growth of mammalian cells. Science 140: 802–803
Gerschenson, L. E., I. Harary, and J. G. Mead. 1967. Studiesin vitro on single beating rat heart cells. X. The effect of linoleic and palmitic acids on beating and mitochondrial phosphorylation. Biochim. Biophys. Acta 131: 50–58.
Jenkin, H. M., and L. E. Anderson. 1970. The effect of oleic acid on the growth of monkey kidney cells (LLC-MK2). Exp. Cell Res. 59: 6–10.
Holmes, R., J. Helms, and G. Mercer. 1969. Cholesterol requirement of primary diploid human fibroblasts. J. Cell Biol. 42: 262–271.
Rothblat, G. H. 1972. Cellular sterol metabolism. In: G. H. Rothblat, and V. J. Cristofalo (Eds.),Growth, Nutrition, and Metabolism of Cells in Culture. Vol. 1. Academic Press, New York, 297–325.
Pierson, R. W., Jr., and H. M. Temin. 1972. The partial purification from calf serum of a fraction with multiplication-stimulating activity for chicken fibroblasts in culture and with non-suppressible insulin-like activity. J. Cell. Physiol. 79: 319–330.
Dulak, N. C., and H. M. Temin. 1973. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153–160.
Dulak, N. C., and H. M. Temin. 1973. Multiplication-stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: a family of small peptides. J. Cell. Physiol. 81: 161–170.
Daughaday, W. H., K. Hall, M. S. Raben, W. D. Salmon, Jr., J. L. Van den Brande, and J. J. Van Wyck. 1972. Somatomedin: proposed designation for sulfation factor. Nature 235: 107.
Gerschenson, L. E., T. Okigaki, M. Andersson, J. Molson, and M. B. Davidson. 1972. Finc structural and growth characteristics of cultured rat liver cells. Exp. Cell Res. 71: 49–58.
Saifer, A., and L. Goldman. 1961. The free fatty acids bound to human serum albumin. J. Lipid Res. 2: 268–270.
Sato, G., J. Clark, M. Posner, H. Leffert, D. Paul, M. Morgan, and C. Colby. 1971 Enrichment culture techniques for selection of cell culture strains with desirable physiological and genetic properties. In: E. Diczfalusy (Ed.), In Vitro Methods in Reproductive Cell Biology, Third Karolinska Symposia on Research Methods in Reproductive Endocrinology. Acta Endocrinol. 153: (Suppl.), 126–131.
Sato, G. 1972. Hormone dependent animal cell strains in culture. In: M. Sussman (Ed.),Molecular Genetics and Developmental Biology. Prentice-Hall, Inc., Englewood Cliffs, N. J. pp. 475–481.
Rutter, W. J., R. L. Pictet, and P. W. Morris. 1973. Toward molecular mechanisms of developmental processes. Annu. Rev. Biochem. 42: 601–646.
Eagle, H. 1973. The effect of environmental pH on the growth of normal and malignant cells. J. Cell. Physiol. 82: 1–8.
Eagle, H. 1971. Buffer combinations for mammalian cell culture. Science 174: 500–503.
Ham, R. G. 1972. Cloning of mammalian cells. In: D. M. Prescott (Ed.),Methods in Cell Physiology. Vol. 5. Academic Press, New York, 37–74.
Ling, C. T., G. O. Gey, and V. Richters. 1968. Chemically characterized concentrated corodies for continuous cell culture (the 7C's culture media). Exp. Cell Res. 52: 469–489.
Birch, J. R., and S. J. Pirt. 1970. Improvements in a chemically defined medium for the growth of mouse cells (strain LS) in suspension. J. Cell Sci. 7: 661–670.
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Ham, R.G. Nutritional requirements of primary cultures a neglected problem of modern biology. In Vitro 10, 119–129 (1974). https://doi.org/10.1007/BF02615345
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DOI: https://doi.org/10.1007/BF02615345