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Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture

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Summary

The characteristics of normal mammary epithelial and 7,12-dimethylbenz[a]anthracene (DMBA)-induced adenocarcinoma cells derived from rats and grown in monolayer culture were compared. Normal mammary epithelial cells exhibited different morphology and agglutinability by plant lectins, slower growth rate, and lower saturation density and cloning efficiency. In addition, the normal cells were sensitive to the toxic effect of DMBA, and were unable to grow in soft agar or to form tumors, when inoculated into newborn Sparague-Dawley rats. The converse was true in each case for the adenocarcinoma cells.

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Authors and Affiliations

  1. Naylor Dana Institute for Disease Prevention, American Health Foundation, 10021, New York, New York

    Leonard A. Cohen, Julian Tsuang & Po C. Chan

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  1. Leonard A. Cohen
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  2. Julian Tsuang
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  3. Po C. Chan
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Supported by Public Health Service Research Grant CA 01237603 from the National Cancer Institute

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Cohen, L.A., Tsuang, J. & Chan, P.C. Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture. In Vitro 10, 51–62 (1974). https://doi.org/10.1007/BF02615338

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