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Hydrocarbon-metabolizing activity of various mammalian cells in culture

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Summary

Two methods for determining the hydrocarbon-metabolizing enzyme activity of cultured mammalian cells were compared. The method designed to measure benzo[a]an-thracene-induced aryl hydrocarbon hydroxylase activity could detect and quantify enzyme activities in low passage rodent cells, but could not reproducibly detect levels in intermediate or high passage mouse, rat, or human cells. The method designed to measure the ability of a cell to convert benzo[a]pyrene from an organic-soluble to an aqueous acetone-soluble form proved to be more reproducible. This technique, when modified, was demonstrated to be an effective screening test for the detection of those lines with higher levels of hydrocarbon-metabolizing enzymes.

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Author notes

  1. Raymond Kiefer & Eugene M. Zimmerman

    Present address: , Litton-Bionetics, 20795, Kensington, Md

Authors and Affiliations

  1. Department of Viral-Chemical Oncology, Microbiological Associates, 20014, Bethesda, Maryland

    Richard E. Kouri, Raymond Kiefer & Eugene M. Zimmerman

Authors
  1. Richard E. Kouri
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  2. Raymond Kiefer
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  3. Eugene M. Zimmerman
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Additional information

Supported by the Council for Tobacco Research and Contract NIH 70-2068 within the Virus Cancer Program, National Cancer Institute, National Institutes of Health.

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Kouri, R.E., Kiefer, R. & Zimmerman, E.M. Hydrocarbon-metabolizing activity of various mammalian cells in culture. In Vitro 10, 18–25 (1974). https://doi.org/10.1007/BF02615334

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