Skip to main content
Log in

On the contamination of cell cultures byLeptospira biflexa

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

A contaminant belonging to the genusLeptospira was detected in different cell lines. This organism was isolated and identified asLeptospira biflexa, a saprophytic species commonly inhabiting fesh waters. The source of this contaminant was not ascertained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Minowada, J., T. Ohnuma, and G. E. Moore. 1972. Rosette-forming human lymphoid lines. 1. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891–895.

    PubMed  CAS  Google Scholar 

  2. Wright, B., P. A. O’Brien, G. T. Shibley, S. A. Mayyasi, and J. C. Lasfargues 1967. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 27: 1672–1677.

    PubMed  CAS  Google Scholar 

  3. Vera, H. D., and M. Dumoff. 1970. Culture media. In: J. E. Blair, E. H. Lennette, and J. P. Truant (Eds.),Manual of Clinical Microbiology. American Society for Microbiology, Bethesda, Md., pp. 633–674.

    Google Scholar 

  4. Alexander, A. D. 1970. Leptospira. In: J. E. Blair, E. H. Lennette, and J. P. Truant (Eds.),Manual of Clinical Microbiology. American Society for Microbiology, Bethesda, Md., pp. 244–250.

    Google Scholar 

  5. Johnson, R. C., and P. Rogers. 1964. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88: 1618–1623.

    PubMed  CAS  Google Scholar 

  6. Füsi, M., and R. Cźoka. 1960. Differentiation of pathogenic and saprophytic leptospirae by means of a copper sulfate test. Zentralbl. Bakteriol. Parasitenk. (Orig.) 179: 231–237.

    Google Scholar 

  7. Fogh, J., N. B. Holmgren, and P. P. Ludovici. 1971. A review of cell culture contaminations. In Vitro 7: 26–41.

    PubMed  CAS  Google Scholar 

  8. Armstrong, D., 1973. Contamination of tissue culture by bacteria and fungi. In: J. Fogh (Ed.),Contamination in Tissue Culture, Academic Press, Inc., New York, pp. 51–64.

    Google Scholar 

  9. Merrill, C. R., T. B. Friedman, A. F. Attallah, M. R. Geier, K. Krell, and R. Yarkin. 1972. Isolation of bacteriophages from commercial sera. In Vitro 8: 91–93.

    Google Scholar 

  10. Smibert, R. M. 1973. Spirochetales, a review. In: A. I. Laskin and H. Lechevalier (Eds.),Critical Reviews in Microbiology. Vol. 2. Chemical Rubber Co., Cleveland, Ohio, pp. 491–552.

    Google Scholar 

  11. Dimitroff, V. T. 1927.Leptospira biflexa (Wolbach and Binger, 1914, Emend. Noguchi, 1918). J. Infect. Dis. 40: 508–515.

    Google Scholar 

  12. Brendle, J. J., and A. D. Alexander. 1974. Contamination of bacteriological media byLeptospira biflexa. Appl. Microbiol., in press.

  13. Finn, M. A., and H. M. Jenkin. 1973. Cytopathic effects of Leptospira, serotypes patoc and canicola in three kidney cell culture systems. Am. J. Vet. Res. 34: 669–672.

    PubMed  CAS  Google Scholar 

  14. Ellison, D., J. Rigg, S. J. McConnell, A. D. Alexander, and R. H. Yager. 1965. Use of antibiotics in the preparation of canine tissue culture vaccines to eliminate leptospiral infection hazards. Appl. Microbiol. 13: 595–599.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tumilowicz, J.J., Alexander, A.D. & Stafford, K. On the contamination of cell cultures byLeptospira biflexa . In Vitro 10, 238–242 (1974). https://doi.org/10.1007/BF02615238

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02615238

Key words

Navigation