Summary
The primary objective of this study was to obtain pure cultures of prostatic epithelium. Encapsulation by epithelial cells and hypocellularity in stroma occurred when explants of prostatic tissue were maintained in suspension cultures. Twenty per cent fetal bovine serum incorporated into the medium provided optimal conditions for encapsulation and preservation of epithelial cell viability and architecture. Horse serum at the same concentration was less effective.
When encapsulated explants were allowed to attach to the substrate, 10 and 20% horse serum favored growth of epithelial cells while fetal bovine serum also stimulated fibroblastic growth.
Mechanisms for the induction of hypocellularity, encapsulation, squamous metaplasia and central necrosis in explants were studied. Relationship between the type and concentration of serum and the nature and extent of outgrowth are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Homburger, F., and C. W. Nixon. 1970. Cystic prostatic hypertrophy in two inbred lines of Syrian hamsters. Proc. Soc. Exp. Biol. Med. 134: 284–286.
Brendler, H. 1963. Experimental prostatic cancer: background of the problem. In:Biology of the Prostate and Related Tissues. Natl. Cancer Inst. Monogr. 12: 343–349.
Chen, T. T., and C. Heidelberger. 1969.In vitro transformation of cells derived from mouse prostate in the presence of methylcholanthrene. J. Natl. Cancer Inst. 42: 915–925.
Paulson, D. F., E. E. Fraley, J. B. DeKernion, and A. S. Robson. 1973. Virus-induced genitourinary malignancy—macromolecular identity. Invest. Urol. 10: 298–304.
Fraley, E. E., and S. Ecker. 1971. Tumor production in immune suppressed hamsters by spontaneously transformed human prostatic epithelium. J. Urol. 106: 95–99.
Brehmer, B., H. Marquardt, and P. O. Madsen. 1972. Growth and hormonal response of cells derived from carcinoma and hyperplasia of the prostate in monolayer cell cultures. A possiblein vitro mode for clinical chemotherapy. J. Urol. 108: 890–896.
Schrodt, G. R., and C. D. Foreman. 1971.In vitro maintenance of human hyperplastic prostate tissue. Invest. Urol. 9: 85–94.
Schroeder, F. H., G. Sato, and R. F. Gittes. 1971. Human prostatic adenocarcinoma. Growth in monolayer tissue culture. J. Urol. 106: 734–739.
Stonington, O. G., and H. Hemingson. 1971. Culture of cells as a monolayer derived from the epithelium of the human prostate. A new cell growth technique. J. Urol. 106: 393–400.
Webber, M. M., O. G. Stonington, and J. Lehman. 1973. Virus in prostatic epithelium of man. Urology 1: 561–567.
Webber, M. M. 1975. Ultrastructural changes in human prostatic epithelium grownin vitro. J. Ulstrastruct. Res. 50: in press.
Puck, T. T., S. J. Cieciura, and A. Robinson. 1958. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945–956.
Gaillard, P. J. 1953. Growth and differentiation of explanted tissue. Int. Rev. Cytol. 2: 331–401.
Mostofi, F. K. 1970. Benign hyperplasia of the prostate gland. In: M. F. Campbell and J. H. Harrison (Eds.),Urology. Vol. 2. W. B. Saunders, Philadelphia, pp. 1065–1129.
Lasnitzki, I. 1963. Growth patterns of the mouse prostate glands in organ cultures and its response to sex hormones, vitamin A and 3-methylcholanthrene. In:Biology of the Prostate and Related Tissues. Natl. Cancer Inst. Monogr. 12: 381–403.
Fell, H. B., and L. M. Rinaldini. 1965. The effects of vitamins A and C on cells and tissues in culture. In: E. N. Willmer (Ed.),Cells and Tissue in Culture. Vol. I. Academic Press, New York, pp. 659–699.
Altman, P. L., and D. S. Dittmer (Eds.) 1961.Blood and Other Body Fluids. Federation of the American Societies for Experimental Biology, Washington, p. 89.
Clarke, G. D., and M. G. P. Stoker. 1971. Conditions affecting the response of cultured cells to serum. In: G. E. W. Wolstenholme and J. Knight (Eds.)Growth Control in Cell Cultures. Ciba Foundation Symposium, Churchill Livingston, London, p. 17.
Dulbecco, R. 1970. Topoinhibition and serum requirements of transformed and untrasformed cells. Nature 227: 802–806.
Franks, L. M. 1961. The growth of mouse prostate during culturein vitro in chemically defined and natural media, and after transplantationin vivo. Exp. Cell Res. 22: 56–72.
Holley, R. W., and J. A. Kiernan. 1968. Contact inhibition of cell division in 3T3 cells. Proc. Natl. Acad. Sci. U.S.A. 60: 300–304.
Shodell, M., and H. Rubin. 1970. Studies on the nature of serum stimulation of proliferation in cell culture. In Vitro 6: 66–74.
Temin, H. M., R. W. Pierson, Jr., and R. C. Dulak. 1972. The role of serum in the control of multiplication of avian and mammalian cells in culture. In: G. H. Rothblat and V. J. Cristofalo (Eds.)Growth, Nutrition and Metabolism of Cells in Culture. Vol. I. Academic Press, New York, p. 49.
Todaro, G. J., Y. Matsuya, S. Bloom, A. Robbins, and H. Green. 1967. Stimulation of RNA synthesis and cell division in resting cells by a factor present in serum. In: V. Defendi and M. Stoker (Eds.),Growth Regulating Substances for Animal Cells in Culture. Wistar Inst. Symp. Monogr. 7: 87–101.
Webber, M. M. 1974. Effects of serum on the growth of prostatic cellsin vitro. J. Urol., in press.
Merchant, D. J., R. H. Kahn, and W. H. Murphy. 1964.Handbook of Cell and Organ Culture. Burgess Publishing Co., Minneapolis, p. 238.
Author information
Authors and Affiliations
Additional information
This work was supported in part by the American Cancer Society Grants DT-20 and IN-5N, U. S. Public Health Service Grant RR-05357, the Parke Davis Fund, and the John U. White Fund for Urological Research.
Rights and permissions
About this article
Cite this article
Webber, M.M., Stonington, O.G. & Poché, P.A. Epithelial outgrowth from suspension cultures of human prostatic tissue. In Vitro 10, 196–205 (1974). https://doi.org/10.1007/BF02615233
Issue Date:
DOI: https://doi.org/10.1007/BF02615233