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Rat hepatocyte primary cell cultures

III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium

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Summary

The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of the cultures in various media was compared revealing that in complex media, particularly containing galactose, survival was improved.

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Authors and Affiliations

  1. Naylor Dana Institute for Disease Prevention, American Health Foundation, 1 Dana Road, 10595, Valhalla, New York

    Gary M. Williams, Edilberto Bermudez & Dominick Scaramuzzino

Authors
  1. Gary M. Williams
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  2. Edilberto Bermudez
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  3. Dominick Scaramuzzino
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Additional information

This study was supported by Contract No. N01-CP-55705 from the National Cancer Institute and Research Grant No. BC-133B from the American Cancer Society.

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Williams, G.M., Bermudez, E. & Scaramuzzino, D. Rat hepatocyte primary cell cultures. In Vitro 13, 809–817 (1977). https://doi.org/10.1007/BF02615128

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