Summary
Using chemical transformation of primary monolayers of hamster ventral prostate tissue, we have established the HVP-G3 and HVP-B1 cloned cell lines which have been growing in our laboratories for over 18 months. Both cell lines contain high-affinity, saturable androgen receptors which migrate into the nucleus when exposed to physiological doses of androgen. However, neither cell line demonstrates a growth response to androgens. Each cell line will produce a tumor in 6 to 8 weeks when injected subcutaneously into male or female Syrian hamsters. In vitro cell doubling times range from 12 hr in the presence of luteinizing hormone (LH-NIH-S-18) to 18 hr in the presence of 5% serum. Both cell lines have a diploid stemline.
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This work was supported in part by Grants AM-17307 and HD-07495.
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Norris, J.S., Bowden, C. & Kohler, P.O. Description of a new hamster ventral prostate cell line containing androgen receptors. In Vitro 13, 108–114 (1977). https://doi.org/10.1007/BF02615074
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DOI: https://doi.org/10.1007/BF02615074