Abstract
Flotation rates of the major Sf 0–12 lowdensity lipoprotein component in human serum may be calculated from ultracentrifuge data utilizing two computer programs. One program calculates a classical moving boundary uncorrected flotation rate by a best fit straight line for the points (1n xi, ω2ti). The other program permits correction for concentration dependence and correction to standard reference conditions. Preliminary application of these methods indicates significantly greater flotation rates in normal human females than in males for the 35–49 year age group.
The significance of interrelationships between the serum lipoprotein spectra, the serum lipids and the serum proteins is considered, resulting in the development of a revised method of measuring serum proteins by precision refractometry. The refractometric measurement is corrected in accordance with (any of various) lipid measurements in order to account for the contribution of lipoproteins to the total refractive increment. Such a technique, giving potentially a very accurate protein measurements, has application in screening studies involving abnormalities of both serum lipoprotein and serum protein metabolism.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
deLalla, O., and J. W. Gofman, in “Methods of Biochemical Analysis,” Vol. 1, edited by D. Glick, Interscience Publishers, New York, 1954, p. 459.
Ewing, A. M., N. K. Freeman and F. T. Lindgren, in “Advances of Lipid Research,” Vol. 3, edited by R. Paoletti and D. Kritchevsky, New York, Academic Press, Inc., 1965, Chap. 2, p. 25.
Waugh, D. F., and D. A. Yphantis, Rev. Sci. Instr.23, 609 (1952).
Johnston, J. P., and A. G. Ogston, Trans. Faraday Soc.42, 789 (1946).
Svedberg, T., and K. O. Pederson, “The Ultracentrifuge,” Oxford University Press, London and New York, 1940.
Shachman, H. I., “Ultracentrifugation in Biochemistry,” Academic Press, New York, 1959.
Gropper, L., Anal. Biochem.6, 170 (1963).
Oncley, J. L., K. W. Walton and D. G. Cornwell, J. Am. Chem. Soc.79, 4666 (1957).
Mills, G. L., and P. A. Wilkinson, Clin. Chim. Acta8, 701 (1963).
Zipf, R. E., B. J. Katchman, and F. W. Sunderman, in “Serum Proteins and the Dysproteinemias,” (ed. F. W. Sunderman and F. W. Sunderman, Jr., J. B. Lippincott Co., Philadelphia, Pa., 1964, p. 40.
Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall, J. Biol. Chem.193, 265 (1951).
Gornal, A. G., C. J. Bardawill and M. M. David, J. Biol. Chem.177, 751 (1949).
Williams, F. G., and M. C. Nixon, in “Serum Proteins and the Dysoproteinemias,” ed. F. W. Sunderman and F. W. Sunderman, Jr., J. B. Lippincott Co., Philadelphia, Pa., 1964, p. 110.
deLalla, O. F., University of California Lawrence Radiation Laboratory Report, UCRL-8550, November (1958).
Reiss, E., Inaug, Diss., Strassburg (1902).
Naumann, H. N., in “Serum Proteins and the Dysproteinemias,” ed. F. W. Sunderman and F. W. Sunderman, Jr., J. B. Lippincott Co., Philadelphia, Pa., 1964, p. 86.
Armstrong, S. H., M. J. E. Budka, K. C. Morrison and M. Hasson, J. Am. Chem. Soc.69 1747 (1947).
Hanig, M. and J. R. Shainoff, J. Biol. Chem.219, 479 (1956).
Lindgren, F. T., A. V. Nichols, N. K. Freeman, R. D. Wills, L. Wing and J. E. Gullberg, J. Lipid Res.5, 68 (1964).
Perlmann, G. E. and L. G. Longsworth, J. Am. Chem. Soc.70, 2719 (1948).
Zimm, H., and W. B. Dandliker, J. Phys. Chem.58, 644 (1954).
Heller, W., and T. L. Pugh, J. Coll. Sci.12, 294 (1957).
Bauer, N., K. Fajans and S. Z. Lewin, in “Physical Methods of Organic Chemistry,” 3rd ed., Vol. I, ed. A. Weissberger, Interscience Publishers, New York, 1960, part II, p. 1239.
Sunderman, F. W., J. Biol. Chem.153, 139 (1944).
Drickman, A., and F. A. McKeon, Am. J. Clin. Path.,38, 392 (1962).
Bausch and Lomb, Abee-3L Refractometer, Reference Manual; Serum Protein Meter Reference Manual, Rochester 2, New York (1963).
deLalla, O. F. and J. W. Gofman, in “Blood and Other Body Fluids,” ed. D. S. Dittmer, Washington, D.C., Federation of American Societies for Experimental Biology, 1961, p. 64.
Freeman, N. K., J. Lipid Res. 5, 236 (1964).
Freeman, N. K., E. Lampo and A. A. Windsor, University of California Lawrence Radiation Laboratory Report (in press).
Author information
Authors and Affiliations
About this article
Cite this article
Lindgren, F.T., Freeman, N.K., Ewing, A.M. et al. Serum lipoprotein distribution, flotation rates and protein analysis. J Am Oil Chem Soc 43, 281–285 (1966). https://doi.org/10.1007/BF02609674
Issue Date:
DOI: https://doi.org/10.1007/BF02609674