Skip to main content
SpringerLink
Log in

Intracytoplasmic membrane production inEscherichia coli O111a1: Nutritional parameters

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Escherichia coli O111a1 ceased growth prematurely and accumulated intracytoplasmic membrane at 42°C in an amino acids-mineral salts medium. The amount of membrane formed appeared to be proportional to the concentration of amino acids in the medium—the greater the concentration of amino acids in the medium, the greater the membrane production.E. coli O111a1, did not grow at 42°C in glucose-, glycerol- or acetate-mineral salts medium, but mesosome-like structures were produced in glucose-grown cells and some intracytoplasmic membrane in cells grown on glycerol and acetate. Supplementation of the glucose medium with pantothenate and/or thiamine permitted normal growth. The vitamins did not restore growth of the mutant in glycerol or acetate, but intracytoplasmic membrane production was increased, especially in glycerol. Amino acids plus glucose supported normal growth with no membrane production. Glycerol and acetate had no effect on the growth in the amino acids medium, but stimulated the accumulation of membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Altenburg, B. C., Suit, J. C. 1970. Relation between deoxyribonucleic acid and intracytoplasmic membranes inEscherichia coli O111a1. Journal of Bacteriology103:227–237.

    PubMed  CAS  Google Scholar 

  2. Cousia, D., Kohiyama, M. 1955.Escherichia coli K12 temperature-sensitive mutations, pp. 273–283 In: Proceedings of the Symposia on Mutational Process. Prague. Prague. Academia.

    Google Scholar 

  3. Dennis, C. A., Rogerson, A. C. 1975. Unusual membranous structures in minicells and minicell-producing strains ofEscherichia coli. Journal of Bacteriology124:1610–1612.

    PubMed  CAS  Google Scholar 

  4. DePetris, S. 1967. Ultrastructure of the cell wall ofEscherichia coli and chemical nature of its constiment layers. Journal of Ultrastructure Research19:45–83.

    Article  CAS  Google Scholar 

  5. DiJoseph, C. G., Bayer, M. E., Kaji, A. 1973. Host cell growth in the presence of the thermosensitive drug resistance factor, Rts 1. Journal of Bacteriology115:399–410.

    PubMed  CAS  Google Scholar 

  6. Fischman, D. A., Weinbaum, G. 1967. The formation of multiple layers of membrane-like structures inEscherichia coli B. Journal of Cell Biology32:524–528

    Article  PubMed  CAS  Google Scholar 

  7. Greenawalt, J. W. 1974. The isolation of intracellular membranes ofEscherichia coli Ollla1. Methods in Enzymology31:633–642.

    Article  PubMed  CAS  Google Scholar 

  8. Greenawalt, J.W., Whiteside, T. L. 1975. Mesosomes: Membranous bacterial organelles. Bacteriological Reviews39:405–463.

    PubMed  CAS  Google Scholar 

  9. Grula, E. A., Grula, M. M. 1962. Cell division in species ofErwinia. III. Reversal of inhibition of cell division caused byd-amino acids, penicillin, and ultraviolet light. Journal of Bacteriology83:981–988.

    PubMed  CAS  Google Scholar 

  10. Higgins, M. L., Shockman, G. D. 1970. Early changes in the ultrastructure ofStreptococcus faecalis after amino acid starvation. Journal of Bacteriology103:244–254.

    PubMed  CAS  Google Scholar 

  11. Hirota, Y., Ryter, A., Jacob, F. 1968. Thermosensitive mutants ofE. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harbor Symposia on Quantitative Biology33:677–693.

    PubMed  CAS  Google Scholar 

  12. Kohiyama, M., Cousin, D., Ryter, A., Jacob, F. 1966. Mutants thermosensibles d'Escherichia coli K12. Annales de l'Institut Pasteur, Paris110:465–486.

    CAS  Google Scholar 

  13. Lutsch, G., Venker, P. 1969. Intracytoplasmatische Membranen inE. coli nach Magnesium-bzw. Phosphat-Mangel. Naturwissenschaften56:568.

    Article  CAS  Google Scholar 

  14. Massey, L. I., Sokatch, J. R., Conrad, R. S. 1976. Branchedchain amino acid catabolism in bacteria. Bacteriological Reviews40:42–54.

    PubMed  CAS  Google Scholar 

  15. Morgan, C., Rosenkranz, H. S., Chan, B., Rose, H. M. 1966. Electron microscopy of magnesium-depleted bacteria. Journal of Bacteriology91:891–895.

    PubMed  CAS  Google Scholar 

  16. Ohnishi, Y. 1971. Phospholipids of virus-induced membranes in cytoplasm ofEscherichia coli. Journal of Bacteriology107:918–925.

    CAS  Google Scholar 

  17. Ohnishi, Y., Kuwand, M. 1971. Growth inhibition and appearance of the membranous structure inEscherichia coli infected with bacteriophage fd. Journal of Virology7:673–678.

    Google Scholar 

  18. Pontefract, R. D., Bergeron, G., Thatcher, F. S. 1969. Mesosomes inEscherichia coli. Journal of Bacteriology97:367–375.

    Article  PubMed  CAS  Google Scholar 

  19. Schnaitman, C., Greenawalt, J. W. 1966. Intracytoplasmic membranes inEscherichia coli. Journal of Bacteriology92:780–783.

    PubMed  CAS  Google Scholar 

  20. Singh, M., Dempsey, W. B., Srere, P. A. 1975. Incorporation of pantothenate into citrate lyase by a pantothenateless mutant ofKlebsiella pneumoniae. Jounral of Bacteriology124:686–692.

    CAS  Google Scholar 

  21. Slater, M., Schaecter, M. 1974. Control of cell division in bacteria. Bacteriological Reviews38:199–221.

    PubMed  CAS  Google Scholar 

  22. Smith, K. R., Jr., Smith, G. R. 1960. An electron-microscopic study of methionine deficientEscherichia coli. Journal of Ultrastructure Research4:213–221.

    Article  Google Scholar 

  23. Umbarger, H. E. 1971. The regulation of enzyme levels in the pathways to the branched chain amino acids, pp. 447–462. In: H. E. Vogel (ed.), Metabolic pathways, vol. 5, 3rd ed. New York: Academic Press.

    Google Scholar 

  24. Weigand, R. A. 1972. Extra membranes inEscherichia coli O111a: Their formation, isolation and partial characterization. PhD. dissertation. The Johns Hopkins University Medical School, Baltimore, Maryland.

    Google Scholar 

  25. Weigand, R. A., Holt, S. C., Shively, J. M., Decker, G. L., Greenwalt, J. W. 1973. Ultrastructural properties of extra membranes ofEscherichia coli O111a as revealed by freezefracturing and negative-staining techniques. Journal of Bacteriology113:433–444.

    PubMed  CAS  Google Scholar 

  26. Weigand, R. A., Shively, J. M., Greenwalt, J. W. 1970. Formation and ultrastructure of extra membranes inEscherichia coli. Journal of Bacteriology102:240–249.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Clemson University, 29631, Clemson, South Carolina, USA

    J. W. Brunson, J. M. Shively & Kathleen Stralka

  2. Department of Physiological Chemistry, The Johns Hopkins University Medical School, 21205, Baltimore, Maryland, USA

    G. L. Decker

Authors
  1. J. W. Brunson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. M. Shively
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kathleen Stralka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. L. Decker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. W. Greenawalt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brunson, J.W., Shively, J.M., Stralka, K. et al. Intracytoplasmic membrane production inEscherichia coli O111a1: Nutritional parameters. Current Microbiology 1, 123–128 (1978). https://doi.org/10.1007/BF02605429

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02605429

Keywords

Search

Navigation