Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures
- 114 Downloads
Reproducibility of relative binding ratios (RBR) was studied with 26 labeled DNA-unlabeled DNA hybridization reactions carried out at two temperatures (60 and 75°C) and following three procedures: the S1-nuclease method with precipitation of S1-resistant DNA by trichloroacetic acid (S1-TCA), the S1-nuclease method with adsorption of S1-resistant DNA onto DE81 filters (S1-DE81), and the hydroxyapatite (HA) method. Analyses of variance indicated that different hybridization methods give different RBR, but similar percent divirgence (ΔTm) results. A curvilinear relationship was found between RBR data obtained with S1-TCA and HA procedures at 60 or 75°C, and between 60°C RBR data obtained with S1-DE81 and HA procedures. At 75°C, RBR obtained with S1-DE81 and HA methods are comparable without any transformation of the data.
Unable to display preview. Download preview PDF.
- 4.Cooley, W. W., Lohnes, P. R. 1971. Multivariate data analysis. New York: Wiley.Google Scholar
- 8.Grimont, P. A. D., Grimont, F., Starr, M. P. 1978.Serratia proteamaculans (Paine and Stansfield) comb. nov., a senior subjective synonum ofSerratia liquefaciens (Grimes and Hennerty) Bascomb et al. International Journal of Systematic Bacteriology28:503–510.Google Scholar
- 12.Maniatis, T., Jeffrey, A., Kleid, D. G. 1975. Nucleotide sequence of the rightward operator of phage. Proceedings of the National Academy of Sciences of the United States of America48:1390–1397.Google Scholar
- 18.Sokal, R. R., Rohlf, F. J. 1969. Biometry, the principles and practice of statistics in biological research. Ames, Iowa: Iowa State University Press.Google Scholar