Skip to main content
SpringerLink
Log in

Interspecific protoplast fusion in fission yeasts

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Protoplasts of auxotrophic mutants isolated fromSchizosaccharomyces pombe andSchizosaccharomyces octosporus were fused. The fusion products were capable of complementation and growth on minimal medium, but the morphological observations suggested a disturbed physiological balance, which was reflected in low viability, osmotic sensitivity, and the formation of incomplete cell wall. Their asymmetric segregation indicated thatSchizosaccharomyces octosporus might be dominant in the fused cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Ahkong, A. F., Fischer, D., Tampion, W., Lucy, J. A. 1975. Mechanisms of cell fusion. Nature253:194–195.

    Article  PubMed  CAS  Google Scholar 

  2. Allmark, B. M., Morgan, A. J., Whittaker, P. A. 1978. The use of protoplast fusion in demonstrating chromosomal and mitochondrial inheritance of respiratory-deficiency inKluyveromyces lactis, a petite-negative yeast. Molecular and General Genetics159:297–299.

    Article  Google Scholar 

  3. Anne, J., Peberdy, J. F. 1976. Induced fusion of fungal protoplasts following treatment with polyethylene glycol. Journal of General Microbiology92:413–417.

    PubMed  CAS  Google Scholar 

  4. Ferenczy, L. 1976. Some characteristics of intra- and interspecific protoplast fusion products ofAspergillus nidulans andAspergillus fumigatus, pp. 171–182. In: Dudits, D., Farkas, G. L., Maliga, P. (eds.), Cell genetics in higher plants. Budapest: Akadémiai Kiadó.

    Google Scholar 

  5. Ferenczy, L., Kevei, F., Szegedi, M. 1975. High-frequency fusion of fungal protoplasts. Experientia31:1028–1030.

    Article  CAS  Google Scholar 

  6. Ferenczy, L., Maráz, A. 1977. Transfer of mitochondria by protoplast fusion inSaccharomyces cerevisiae. Nature268:524–525.

    Article  PubMed  CAS  Google Scholar 

  7. Fodor, K., Alföldi, L. 1976. Fusion of protoplasts ofBacillus megaterium. Proceedings of the National Academy of Sciences of the United States of America73:2147–2150.

    Article  PubMed  CAS  Google Scholar 

  8. Fournier, P., Provost, A., Bourguignon, C., Heslot, H. 1977. Recombination after protoplast fusion in the yeastCandida tropicalis. Archives of Microbiology115:143–149.

    Article  PubMed  CAS  Google Scholar 

  9. Kao, K. N., Michayluk, M. R. 1974. A method for high-frequency intergeneric fusion of plant protoplasts. Planta115:355–367.

    Article  CAS  Google Scholar 

  10. Provost, A., Bourguignon, C., Fournier, P., Ribet, A. M., Heslot, H. 1978. Intergeneric hybridization in yeasts through protoplast fusion. FEMS Microbiology Letters3:309–312.

    Article  Google Scholar 

  11. Schaeffer, P., Cami, B., Hotchkiss, R. D. 1976. Fusion of bacterial protoplasts. Proceedings of the National Academy of Sciences of the United of America73:2151–2155.

    Article  CAS  Google Scholar 

  12. Sipiczki, M., Ferenczy, L. 1977. Protoplast fusion ofSchizosaccharomyces pombe auxotrophic mutants of identical mating type. Molecular and General Genetics151:77–81.

    Article  PubMed  CAS  Google Scholar 

  13. Sipiczki, M., Ferenczy, L.. 1977 Fusion ofRhodosporidium (Rhodotorula) protoplasts. FEMS Microbiology Letters2:203–205.

    Article  Google Scholar 

  14. Sipiczki, M., Ferenczy, L.. 1978 Enzymic methods for enrichment of fungal mutants. I. Enrichment ofSchizosaccharomyces pombe mutants. Mutation Research50:163–173.

    CAS  Google Scholar 

  15. Stahl, U. 1978. Zygote formation and recombination between like mating types in the yeastSaccharomycopsis lipolytica by protoplast fusion. Molecular and General Genetics160:111–113.

    Google Scholar 

  16. Svoboda, A. 1978. Fusion of yeast protoplasts induced by polyethylene glyco. Journal of General Microbiology109:169–175.

    Google Scholar 

  17. van Solingen, P., van der Plaat, J. B. 1977. Fusion of yeast protoplasts. Journal of Bacteriology130:946–947.

    PubMed  Google Scholar 

  18. Whittaker, P. A., Leach, S. M. 1978. Interspecific hybrid production between the yeastsKluyveromyces lactis andKluyveromyces fragilis by protoplast fusion. FEMS Microbiology Letters4:31–34.

    Article  Google Scholar 

  19. Yamamoto, M., Fukui, S. 1977. Fusion of yeast protoplasts. Agricultural and Biological Chemistry41:1829–1830.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Attila József University, P.O. Box 428, H-6701, Szeged, Hungary

    M. Sipiczki

Authors
  1. M. Sipiczki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sipiczki, M. Interspecific protoplast fusion in fission yeasts. Current Microbiology 3, 37–40 (1979). https://doi.org/10.1007/BF02603131

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02603131

Keywords

Search

Navigation