Abstract
The primary structure ofTetrahymena thermophila 5S rRNA is reported. A secondary structure model is presented which can encompass most published eukaryotic 5S rRNA sequences. Unlike other eukaryotic 5S rRNAs,Tetrahymena is found to contain the sequence-CGAAC- beginning at position 40. The presence of this segment had previously been thought to be an exclusive characteristic of eubacterial 5S rRNAs.
Similar content being viewed by others
Literature Cited
Bellemare, G., Vigne, R., Jordan, B. R. 1973 Interaction betweenEscherichia coli ribosomal proteins and 5S RNA molecules: Recognition of prokaryotic 5S RNAs and rejection of eukaryotic 5S RNAs. biochimie55:29–35.
Benhamou, J., Jordan, B. R. 1976. Nucleotide sequence ofDrosophila melanogaster 5S RNA—evidence for a general 5S RNA model. FEBS Letters62:146–149.
Brownlee, G. C., Sanger, F., Barrell, B. G. 1968. The sequence of 5S ribosomal ribonucleic acid. Journal of Molecular Biology34:379–412.
Forget, B. G., Weissman, S. M. 1969. The nucleotide sequence of ribosomal 5S ribonucleic acid from KB cells. Journal of Biological Chemistry244:3148–3165.
Fox, G. F., Woese, C. R. 1975. 5S rRNA secondary structure. Nature256:505–507.
Fox, G. E., Woese, C. R. 1975. The architecture of 5S rRNA and its relation to function. Journal of Molecular Evolution6:61–76.
Fox, G. E., Stackebrandt, E., Hespell, R. B., Gibson, J., Maniloff, J., Dyer, T. A., Wolfe, R. S., Balch, W. E., Tanner, R. S., Magrum, L. J., Zablen, L. B., Blakemore, R., Gupta, R., Bonen, L., Lewis, B. J., Stahl, D. A., Luehrsen, K. R., Chen, K. N., Woese, C. R. 1980. The phylogeny of prokaryotes. Science209:457–463.
Holley, R. W., Apgar, J., Everett, G. A., Madison, J. T., Marquissee, M., Merrill, S. H., Penswick, J. R., Zamir, A. 1965. A structure of a ribonucleic acid. Science147:1462–1465.
Hori, H. 1976. Molecular evolution of 5S rRNA. Molecular and General Genetics145:119–123.
Hori, H., Osawa, S. 1979. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proceedings of the National Academy of Sciences of the United States of America76:381–385.
Jordan, B. R., Galling, F., Jordan, R. 1974 Sequence and conformation of 5S RNA fromChlorella cytoplasmic ribosomes: Comparison with other 5S RNA molecules. Journal of Molecular Biology87:205–225.
Komiya, H., Takemura, S. 1979. Nucleotide sequence of 5S ribosomal RNA from rainbow trout (Salmo gairdnerii) liver. Journal of Biochemistry86:1067–1080.
Leich, V., Engberg, J., Emmersen, J. 1970. Nascent subribosomal particles inTetrahymena pyriformis. European Journal of Biochemistry13:238–246.
Miyazaki, M. 1977. Nucleotide sequence characterization of 5S ribosomal RNA from several yeast species. Nucleic Acid Research SIS3:S153-S156.
Nanney, D. L., McCoy, J. W. 1976. Characterization of species ofTetrahymena-pyriformis complex. Transactions of the American Microscopical Society95:664–682.
Nishikawa, K., Takemura, S. 1974. Structure and function of 5S ribosomal ribonucleic acid fromTorulopsis utilis: Partial digestion with ribonucleases and derivation of complete sequence. Journal of Biochemistry76:935–947.
Sogin, S. J., Sogin, M. L., Woese, C. R. 1972. Phylogenetic measurement in prokaryotes by primary structural characterization. Journal of Molecular Evolution1:173–184.
Uchida, T., Bonen, L., Schaup, H. W., Lewis, B. J., Zablen, L., Woese, C. 1974. The use of ribonuclease U2 in RNA sequence determination: Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion ofEscherichia coli 16 S ribosomal RNA. Journal of Molecular Evolution3:63–77.
Woese, C. R., Gibson, J., Fox, G. E. 1980. Do genealogical patterns in purple photosynthetic bacteria reflect interspecific gene transfer. Nature283:212–214.
Woese, C. R., Pribula, C. D., Fox, G. E., Zablen, L. 1975. Nucleotide sequence of the 5S ribosomal RNA from a photobacterium. Journal of Molecular Evolution5:35–46.
Woese, C., Sogin, M., Stahl, D., Lewis, B. J., Bonen, L. 1976. A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: Some modifications in the Sanger method for RNA sequencing. Journal of Molecular Evolution7:197–213.
Woese, C. R., Sogin, M. L., Sutton, L. A. 1974. Procaryotic phylogeny: Concerning the relatedness ofAerobacter aerogenes toEscherichia coli. Journal of Molecular Evolution3: 293–299.
Wrede, P., Erdmann, V. A. 1973. Activities ofB. stearothermophilus 50 S ribosomes reconstituted with prokaryotic and eukaryotic 5S RNA. FEBS Letters33:315–319.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luehrsen, K.R., Fox, G.E. & Woese, C.R. The sequence ofTetrahymena thermophila 5S ribosomal ribonucleic acid. Current Microbiology 4, 123–126 (1980). https://doi.org/10.1007/BF02602905
Issue Date:
DOI: https://doi.org/10.1007/BF02602905