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Effect of trypsinization on susceptibility of primary human amniotic cells toChlamydia trachomatis TW-3 strain

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Abstract

The effect of trypsinization of human amnion membranes on the susceptibility of amnion cells toChlamydia trachomatis TW-3 infection was examined by infectivity titrations using standard procedures of chlamydial inoculation, and detection of chlamydial inclusions. Epithelial cells derived from freshly trypsinized membranes as well as primary and secondary cultured cells that were freshly removed from monolayers by trypsin treatment were not susceptible to infection at 30 min and at 2 and 6 h after trypsinization. Monolayers grown 18 h and up to 5 or more days after trypsinization were susceptible to infection. Primary 5-day monolayers derived from each of nine placentas inoculated with chlamydiae showed a range of titers from 10−3 to 10−6.5 (SD=1.2 logarithm). Primary monolayers supported the multiplication of chlamydiae to consistently higher titers than secondary and tertiary monolayers from the same amnion.

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Authors and Affiliations

  1. Department of Medical Microbiology, The Univesity of Manitoba, R3E 0W3, Winnipeg, Manitoba, Canada

    Nonna Kordová & John C. Wilt

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  1. Nonna Kordová
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  2. John C. Wilt
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Kordová, N., Wilt, J.C. Effect of trypsinization on susceptibility of primary human amniotic cells toChlamydia trachomatis TW-3 strain. Current Microbiology 4, 27–30 (1980). https://doi.org/10.1007/BF02602887

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