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Disturbance of lymphocyte circulation byPseudomonas aeruginosa infection in oxazolone-sensitized mice

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Abstract

Pseudomonas aeruginosa infection depresses contact sensitivity to oxazolone in mice. To test whether an altered lymphocyte circulation plays a role in this depression51Cr-labeled lymphocytes fromP. aeruginosa-infected and oxazolone-sensitized donors were injected intravenously into infected and sensitized recipients, and the radioactivity uptake of several organs was determine. The controls consisted of normal mice receiving labeled lymphocytes from normal donors. While the radioactivity recovered from the liver, spleen, and mesenteric lymph nodes was similar in the test and the control group, significantly more radioactivity was recovered from the draining lymph nodes of infected and sensitized recipients. The concentration of labeled lymphocytes from sensitized donors in the draining lymph nodes of sensitized recipients was 18% greater than that of the controls but 31% lower than that of infected and sensitized animals receiving cells from infected and sensitized donors.P. aeruginosa infection enhances lymphocyte entrapment within the draining lymph nodes of oxazolone-sensitized mice.

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Literature Cited

  1. Asherson, G. L. 1977. Depression of cell-mediated immunity by pretreatment with adjuvants, pp. 382–387. In: Schlesinger, D. (ed.), Microbiology— 1977. Washington, D.C.: American Society for Microbiology.

    Google Scholar 

  2. Bainbridge, D. R., Brent, L., Gowland, G. 1966. Distribution of allogeneic51Cr-labeled lymph node cells in mice. Transplantation4:138–153.

    Article  PubMed  CAS  Google Scholar 

  3. Bartell, P. F., Orr, T. E., Gargia, M. 1968. The lethal events in experimentalPseudomonas aeruginosa infection in mice. Journal of Infectious Diseases118:165–172.

    PubMed  CAS  Google Scholar 

  4. Bliss, C. I. 1976. Statistics in biology. New York: McGraw-Hill.

    Google Scholar 

  5. Brissette, W. H., Coleman, R. M., Rencricca, N. J. 1978. Depressed splenic T lymphocyte numbers and thymocyte migratory patterns in murine malaria. Proceeding of the Society for Experimental Biology and Medicine159:317–320.

    CAS  Google Scholar 

  6. Bulloch, W. E. 1976. Perturbation of lymphocyte circulation in experimental murine leprosy. II. Nature of the defect. Journal of Immunology117:1171–1178.

    Google Scholar 

  7. Campa, M., Ferrannini, E., Colizzi, V., Garzelli, C. 1977. Distribution of51Cr-labeled lymph node cells inPseudomonas aeruginosa-infected mice. Journal of Nuclear Medicine and Allied Sciences21:37–42.

    PubMed  CAS  Google Scholar 

  8. Campa, M., Garzelli, C., Falcone, G. 1975. Depression of contact sensitivity and enhancement of antibody response inPseudomonas aeruginosa-infected mice. Infection and Immunity12:1252–1257.

    PubMed  CAS  Google Scholar 

  9. Campa, M., Garzelli, C., Ferrannini, E., Falcone, G. 1976. Evidence for suppressor cell activity associated with depression of contact sensitivity inPseudomonas aeruginosa-infected mice. Clinical and Experimental Immunology26:355–362.

    PubMed  CAS  Google Scholar 

  10. Colizzi, V., Garzelli, C., Campa, M., Falcone, G. 1978. Depression of contact sensitivity by enhancement of suppressor cell activity inPseudomonas aeruginosa-infected mice. Infection and Immunity21:354–359.

    PubMed  CAS  Google Scholar 

  11. Floersheim, G. L., Hopff, W. H., Gasser, M., Bucher, K. 1971. Impairment of cell mediated immune responses byPseudomonas aeruginosa. Clinical and Experimental Immunology9:241–247.

    PubMed  CAS  Google Scholar 

  12. Freitas, A. A., de Sousa, M. 1977. Control mechanism of lymphocyte traffic. A study of the action of two sulfated polysaccharides on the distribution of51Cr- and [3H]adenosine-labeled mouse lymph node cells. Cellular Immunology31:62–76.

    Article  PubMed  CAS  Google Scholar 

  13. Frost, P., Lance, E. M. 1978. On the mechanism of action of adjuvants. Immunology35:63–68.

    PubMed  CAS  Google Scholar 

  14. Hall, J. G., Morris, B. 1962. The output of cells in lymph from the popliteal node of sheep. Quarterly Journal of Experimental Physiology47:360–369.

    CAS  Google Scholar 

  15. Sasaki, S. 1967. Production of lymphocytosis by polysaccharide polysulphate. (Heparinoids). Nature214:1041–1042.

    Article  PubMed  CAS  Google Scholar 

  16. Spry, C. J. F. 1972. Inhibition of lymphocyte recirculation by stress and corticotropin. Cellular Immunology4:86–92.

    Article  PubMed  CAS  Google Scholar 

  17. Stone, H. H., Given, K. S., Martin, J. D. 1967. Delayed rejection of skin homografts inPseudomonas sepsis. Surgery Gynecology and Obstetrics124:1067–1070.

    CAS  Google Scholar 

  18. Taub, R. H., Rosett, W., Adler, A., Morse, S. I. 1972. Distribution of labeled lymph node cells in mice during the lymphocytosis induced byBordetella pertussis. Journal of Experimental Medicine136:1581–1593.

    Article  PubMed  CAS  Google Scholar 

  19. Woodruff, M. F. A., Nolan, B., Robson, J. S., MacDonald, M. K. 1969. Renal transplantation in man. Lanceti:6–12.

    Article  Google Scholar 

  20. Woodruff, J. J., Woodruff, J. F. 1974. Virus-induced alterations of lymphoid tissues. IV. The effect of Newcastle disease virus on the fate of radiolabeled thoracic duct lymphocytes. Cellular Immunology10:78–85.

    Article  PubMed  CAS  Google Scholar 

  21. Zatz, M. M., Lance, E. M. 1971. The distribution of51Cr-labeled lymphocytes into antigen-stimulated mice. Journal of Experimental Medicine134:224–241.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Istituto di Microbiologia, Universitá di Pisa, Via S. Zeno, 39, 56100, Pisa, Italy

    Mario Campa, Carlo Garzelli, Vittorio Colizzi & Giuseppe Falcone

  2. Laboratorio di Fisiologia Clinica del C.N.R., Via Savi, 8, 56100, Pisa, Italy

    Eleuterio Ferrannini

Authors
  1. Mario Campa
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  2. Eleuterio Ferrannini
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  3. Carlo Garzelli
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  4. Vittorio Colizzi
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  5. Giuseppe Falcone
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Campa, M., Ferrannini, E., Garzelli, C. et al. Disturbance of lymphocyte circulation byPseudomonas aeruginosa infection in oxazolone-sensitized mice. Current Microbiology 2, 283–286 (1979). https://doi.org/10.1007/BF02602860

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