Abstract
A number ofBacillus subtilis mutants that are able to sporulate in the presence of relatively high concentrations of carbon sources have been isolated in our laboratory. The present study shows that some of the mutants are also able to sporulate in the presence of nalidixic acid (Nal) under the condition where sporulation of wild-type strains is inhibited. Furthermore, it has been found that a Nal-resistant mutant is unable to sporulate normally when Nal (40 μg/ml) or glucose (55 mM) is present. Since the adverse effect of Nal on inducible enzymes is eliminated when bacterial strains carry a Nal-resistance marker, the above result suggests that the effect of this drug on sporulation might be mediated by a unique mechanism.
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Literature Cited
Cook, T. M., Brown, K. G., Boyle, J. V., Goss, W. A. 1966. Bactericidal action of nalidixic acid onBacillus subtilis. Journal of Bacteriology92:1510–1514.
Freese, E., Klofat, W., Gallier, E. 1970. Commitment to sporulation and induction of glucose-phosphoenolpyruvate-transferase. Biochimica et Biophysica Acta222:265–289.
Gellert, M., O’Dea, M. H., Itoh, T., Tomizawa, J. 1976. Novobiocin and courmermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proceedings of National Academy of Sciences of the United States of America73:4474–4478.
Ginsberg, D., Keynan, A. 1978. Independence ofBacillus subtilis spore outgrowth from DNA synthesis. Journal of Bacteriology136:111–116.
Sankaran, L., Pogell, B. M. 1973. Differential inhibition of catabolite sensitive enzyme induction by intercalating dyes. Nature New Biology245:257–260.
Sanzey, B. 1979. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase. Journal of Bacteriology138:40–47.
Schaeffer, P., Millet, J., Aubert, J.-P. 1965. Catabolic repression of bacterial sporulation. Proceedings of National Acacemy of Sciences of the United States of America54:704–711.
Shuman, H., Schwartz, M. 1975. The effect of nalidixic acid on the expression of some genes inEscherichia coli K-12. Biochemical and Biophysical Research Communications64:204–209.
Sugino, A., Peebles, C. L., Kreuzer, K. N., Cozzarelli, N. R. 1977. Mechanism of action of nalidixic acid: Purification ofEscherichia coli nal A gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme. Proceedings of National Academy of Sciences of the United States of America74:4767–4771.
Takahashi, I. 1961. Genetic transduction inBacillus subtilis. Biochemical and Biophysical Research Communications5:171–175.
Takahashi, I. 1979. Catabolite repression-resistant mutants ofBacillus subtilis. Canadian Journal of Microbiology25:1283–1287.
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Takahashi, I. Effect of nalidixic acid on sporulation of catabolite-resistant mutants ofBacillus subtilis . Current Microbiology 4, 159–161 (1980). https://doi.org/10.1007/BF02602821
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DOI: https://doi.org/10.1007/BF02602821