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Analysis of glucose catabolites by using head-space gas chromatography for differentiation of some Gram-negative species

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Abstract

Volatile products from the degradation of glucose by a total of 66 strains ofEnterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, andPseudomonas aeruginosa were analyzed by head-space gas chromatography. The bacteria were incubated for 1 h in a glucose-containing buffer solution before being analyzed by gas chromatography, using manual, semiautomatic, and automatic head-space injection. From the chromatographic patterns obtained, all strains could be differentiated as to species, with the exception of two strains ofProteus mirabilis, which gave chromatograms similar to those produced byProteus vulgaris. The gas chromatographic head-space technique developed provides a rapid and easily performed means for identification of bacteria, particularly when using an automatic injection unit, as exemplified in this study on some of the Gram-negative species commonly encountered in urinary tract infections.

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Authors and Affiliations

  1. Department of Medical Microbiology, University of Lund, Sölvegatan 23, S-223 62, Lund, Sweden

    Lennart Larsson & Per-Anders M»rdh

  2. Laboratory of Ecological Chemistry, University of Lund, Sweden

    Göran Odham

Authors
  1. Lennart Larsson
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  2. Per-Anders M»rdh
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  3. Göran Odham
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Larsson, L., M»rdh, PA. & Odham, G. Analysis of glucose catabolites by using head-space gas chromatography for differentiation of some Gram-negative species. Current Microbiology 4, 143–146 (1980). https://doi.org/10.1007/BF02602817

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