Skip to main content
SpringerLink
Log in

Fine mapping of the human pentraxin gene region on chromosome 1q23

  • Original Paper
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The 1q21 to 25 region of human chromosome 1 contains genes which encode proteins with immune- and inflammation-associated functions. These include the pentraxin genes, for C-reactive protein (CRP), serum amyloid P (SAP) protein (APCS), and a CRP pseudogene (CRPP1). The region of chromosome 1 containing this cluster is syntenic with distal mouse chromosome 1. We constructed an approximately 1.4 megabase yeast artificial chromosome (YAC) contig with the pentraxin genes at its core. This four-YAC contig includes other genes with immune functions including theFCER1A gene, which encodes the α-subunit of the IgE high-affinity Fc receptor and theIFI-16 gene, an interferon-γ-induced gene. In addition, it contains the histoneH3F2 andH4F2 genes and the gene for erythroid α-spectrin (SPTA1). The gene order is cen.-SPTA1-H4F2-H3F2-IFI-16-CRP-CRPP1-APCS-FCER1A-tel. The contig thus consists of a cluster of genes whose products either have immunological importance, bind DNA, or both.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. Basic local alignment search tool.J Mol Biol 215: 403–410, 1990

    PubMed  CAS  Google Scholar 

  • Anand, R., Riley, J. H., Butler, R., Smith, J. C., and Markham, A. F. A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.Nucleic Acids Res 18: 1951–1956, 1990

    Article  PubMed  CAS  Google Scholar 

  • Baltz, M. L., deBeer, F. C., Feinstein, A., Munn, E. A., Milstein, C. P., Fletcher, T. C., March, J. F., Taylor, J., Bruton, C., Clamp, J. R., Davies, A. J. S., and Pepys, M. B. Phylogenetic aspects of C-reactive protein and related proteins.Ann NY Acad Sci 398: 49–75, 1982

    Article  Google Scholar 

  • Benn, P. A. and Perle, M. A. Chromosome staining and banding techniques.In D. E. Rooney and B. H. Czepulkowski (eds.):Human Cytogenetics: A Practical Approach, pp. 106–109, IRL Press, Oxford, 1992

    Google Scholar 

  • Bodmer, B. and Siboo, R. Isolation of mouse C-reactive protein from liver and serum.J Immunol 118: 1086–1089, 1977

    PubMed  CAS  Google Scholar 

  • Brevario, F., d'Aniello, E. M., Golay, J., Peri, G., Bottazi, B., Bairoch, A., Saccone, S., Marzella, R., Predazzi, V., Rocchi, M., Della Valle, G., Dejana, E., Mantovani, A., and Introna, M. Interleukin-1 inducible genes in endothelial cells.J Biol Chem 267: 22190–22197, 1992

    Google Scholar 

  • Burke, D. T., Carle, G. F., and Olson, M. V. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.Science 236: 806–812, 1987

    Article  PubMed  CAS  Google Scholar 

  • Castano, E. M. and Frangione, B. Biology of disease: human amyloidosis, Alzheimer Disease and related disorders.Lab Invest 58: 122–131, 1988

    PubMed  CAS  Google Scholar 

  • Claus, D. R., Siegel, J., Petras, K., Skour, D., Osmond, A., and Gewurz, H. Complement activation by inteaction of multiple polyanions and polycations in the presence of C-reactive protein.J Immunol 118: 83–87, 1977

    PubMed  CAS  Google Scholar 

  • DeBeer, F. C., Baltz, M. L., Holford, S., Feinstein, A., and Pepys, M. B. Fibronectin and C4-binding protein are selectively bound by aggregated amyloid P component.J Exp Med 154: 1134–1149, 1981

    Article  CAS  Google Scholar 

  • DeWit, T. P. M., Suijkerbuijk, R. F., Capel, P. J. A., van Kessel, A. G., and van der Winkel, J. G. J. Assignment of three human high-affinityFcγ receptor I genes to chromosome 1, band q21.1.Immunogenetics 38: 57–59, 1993

    Article  CAS  Google Scholar 

  • DuClos, T. W., Zlock, L. T., and Rubin, R. L. Analysis of the binding of C-reactive protein to histones and chromatin.J Immunol 141: 4266–4278, 1988

    CAS  Google Scholar 

  • Eckert, R. L. and Green, H. Structure and evolution of the human involucrin gene.Cell 46: 583–589, 1986

    Article  PubMed  CAS  Google Scholar 

  • Ferrari, S., Calabretta, B., de Riel, J. K., Battini, R., Ghezzo, F., Lauret, E., Griffin, C., Emanuel, B. S., Gurrieri, F., and Baserga, R. Structural and functional analysis of a growth-regulated gene, the human calcyclin.J Biol Chem 262: 8325–8332, 1987

    PubMed  CAS  Google Scholar 

  • Fiedel, B. A. Influence of tuftsin-like synthetic peptide derived from C-reactive protein (CRP) on platelet behaviour.Immunology 64: 487–493, 1988

    PubMed  CAS  Google Scholar 

  • Floyd-Smith, G., Whitehead, A. S., Colten, H. R., and Francke U. The human C-reactive protein gene (CRP) and serum amyloid P component gene (APCS) are located on the proximal long arm of chromosome 1.Immunogenetics 24: 171–176, 1986

    PubMed  CAS  Google Scholar 

  • Goldman, N. D., Liu, T., and Lei, K. Structural analysis of the locus containing the human C-reactive protein gene and its related pseudogene.J Biol Chem 262: 7001–7005, 1987

    PubMed  CAS  Google Scholar 

  • Green, L., Antwerpen, V., Stein, J., and Stein, G. A major human histone gene cluster on the long arm of chromosome 1.Science 226: 838–840, 1984

    Article  PubMed  CAS  Google Scholar 

  • Hamazaki, H. Calcium-dependent binding of human serum amyloid P component to Alzheimer's β-amyloid peptide.J Biol Chem 270: 10392–10394, 1995

    PubMed  CAS  Google Scholar 

  • Hardas, B. D., Zhang, J., Trent, J. M., and Elder, J. T. Direct evidence for homologous sequences on the paracentric regions of human chromosome 1.Genomics 21: 359–363, 1994

    Article  PubMed  CAS  Google Scholar 

  • Hawkins, P. N. and Pepys, M. B.In M. B. Pepys (ed.): Acute Phase Proteins in the Acute Phase Response, pp. 187–206, Springer, London, 1989

    Google Scholar 

  • Hicks, P. S., Saunero-Nava, L., DuClos, T. W., and Mold, C. Serum amyloid P binds to histones and activates the classical complement pathway.J Immunol 149: 3689–3694, 1992

    PubMed  CAS  Google Scholar 

  • Hupp, K., Siwarski, D., Mock, B. A., and Kinet, J. Gene mapping of the three subunits of the high affinity FcR for IgE to mouse chromosomes 1 and 19.J Immunol 143: 3787–3791, 1989

    PubMed  CAS  Google Scholar 

  • Jones, D. A., Carlton, D. P. D., McIntyre, T. M., Zimmerman G. A., and Prescott, S. M. Molecular cloning of human prostaglandin endoperoxide synthase type II and demonstration of expression in response to cytokines.J Biol Chem 268: 9049–9054, 1993

    PubMed  CAS  Google Scholar 

  • Kalaria, R. N., Galloway, P. G., and Perry, G. Widespread serum amyloid P immunoreactivity in cortical amyloid deposits and the neurofibrillary pathology of Alzheimer's disease and other degenerative disorders.Neuropathol Appl Neurobiol 17: 189–201, 1991

    PubMed  CAS  Google Scholar 

  • Kilpatrick, J. M. and Volanakis, J. E. Opsonic properties of C-reactive protein. Stimulation by phorbolmyristate acetate enables human neurophils to phagocytize C-reactive protein covered cells.J Immunol 134: 3364–3370, 1985

    PubMed  CAS  Google Scholar 

  • Kingsmore S. F., Watson, M. L., Howard, T. A., and Seldin, M. F. A 6000 kb segment of chromosome 1 is conserved in human and mouse.EMBO J 8: 4073–4080, 1989

    PubMed  CAS  Google Scholar 

  • Kochan, J. L. F., Pettine, J., Hakimi, J., Kishi, K., and Kinet, J. P. Isolation of the gene coding for the alpha-subunit of the human high affinity IgE receptor.Nucleic Acids Res 16: 3584, 1988

    Article  PubMed  CAS  Google Scholar 

  • Kushner, I. The acute phase response: an overview.Meth Enzymol 163: 373–383, 1982

    Article  Google Scholar 

  • Lee, T. H., Lee, G. W., Ziff, E. B., and Vilcek, J. Isolation and characterisation of eight tumour necrosis factor induced gene sequences from human fibroblasts.Mol Cell Biol 10: 1982–1988, 1990

    PubMed  CAS  Google Scholar 

  • Linnenbach, A. J., Speicher, D. W., Marchesi, V. T. and Forget, B. G. Cloning of a portion of the chromosomal gene for human erythrocyte α-spectrin by using a synthetic gene fragment.Proc Natl Acad Sci USA 83: 2397–2401, 1986

    Article  PubMed  CAS  Google Scholar 

  • Marashi, F., Helms, S., Shiels, A., Silverstein, A., Greenspan, D. S., Stein, G., and Stein, J. Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5′ regulatory sequences.Biochem Cell Biol 64: 277–289 1986

    Article  PubMed  CAS  Google Scholar 

  • Martin, L. H., Calabi, F., Lefebvre, F.-A., Bilsland, C. A. G., and Milstein, C. Structure and expression of the human thymocyte antigens CD1a, CD1b and CD1c.Proc Natl Acad Sci USA 84: 9189–9193, 1987

    Article  PubMed  CAS  Google Scholar 

  • Mendez, M. J., Scott, P., Erickson, P. B., Drabkin, H. A., and Gemmill, R. M.In D. Nelson and B. Brownstein (eds.):YAC Libraries: A User's Guide, pp. 57–91, W. H. Freeman Press, New York, 1994

    Google Scholar 

  • Morley, J. J. and Kushner, I. Serum C-reactive protein levels in disease.Ann NY Acad Sci. 389: 406–418, 1982

    Article  PubMed  CAS  Google Scholar 

  • Mortensen, R. F., Beisel, K., Zeleznik, N. J., and Le, P. T. Acute phase reactants of mice. II. Strain dependence of serum amyloid-P component (SAP) levels and response to inflammation.J Immunol 130: 885–889, 1983

    PubMed  CAS  Google Scholar 

  • Moseley, W. S. and Seldin, M. F. Definition of mouse chromosome 1 and 3 gene linkage groups that are conserved on human chromosome 1: evidence that a conserved linkage group spans the centromere of human chromosome 1.Genomics 5: 899–905, 1989

    Article  PubMed  CAS  Google Scholar 

  • Moseley, W. S., Watson, M. L., Kingsmore, S. F., and Seldin, M. F.CD1 defines conserved linkage group border between human chromosome 1 and mouse chromosomes 1 and 3.Immunogenetics 30: 378–382, 1989

    Article  PubMed  CAS  Google Scholar 

  • Noland, T. D., Friday, B. B., Maulit, M. T., and Gerton, G. L. The sperm acrosomal matrix contains a novel member of the pentaxin family of calcium-dependent binding proteins.J Biol Chem 269: 32607–32614, 1994

    PubMed  CAS  Google Scholar 

  • Ohnishi, S., Maeda, S., Shimada, K., and Arao, T. Isolation and characterisation of the complete complementary and genomic DNA sequences of human serum amyloid P component.J Biochem 100: 849–858, 1986

    PubMed  CAS  Google Scholar 

  • Oliviera, E. B., Gotschlich, E. C., and Liu, T.-Y. Comparative studies on the binding properties of human and rabbit C-reactive proteins.J Immunol 124: 1396–1402, 1980

    Google Scholar 

  • Osmand, A. P., Friedenson, B., Gewurz, H., Painter, R. H., Hofmann, T., and Shelton, E.. Characterisation of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying pentameric symmetry (pentraxins).Proc Natl Acad Sci USA 74: 739–743, 1977

    Article  PubMed  CAS  Google Scholar 

  • Peltz, G. A., Grundy, H. O., Lebo, R. V., Yssel, H., Barsh, G. S., and Moore, K. W., Human FcγRIII: cloning, expression and identification of the chromosomal locus of two Fc receptors for IgG.Proc Natl Acad Sci USA 86: 1013–1017, 1989

    Article  PubMed  CAS  Google Scholar 

  • Pepys, M. B., Baltz, M., Gomer, K., Davis, A. J. S., and Doenhoff, M. Serum amyloid-P component is an acute phase reactant in mouse.Nature 278: 259–261, 1979

    Article  PubMed  CAS  Google Scholar 

  • Pepys, M. B. and Butler, P. J. G. Serum amyloid P-component is the major calcium dependent specific DNA binding protein of the serum.Biochem Biophys Res Commun 148: 308–313, 1987

    Article  PubMed  CAS  Google Scholar 

  • Pinkel, S., Straume, T., and Gray, J. W. Cytogenetic analysis using quantitative, high sensitivity fluorescence hybridisation.Proc Natl Acad Sci USA 83: 2934–2938, 1986

    Article  PubMed  CAS  Google Scholar 

  • Reid, M. S. and Blobel, C. P. Apexin, an acrosomal pentraxin.J Biol Chem 269: 32615–32620, 1994

    PubMed  CAS  Google Scholar 

  • Riley, J., Butler, R., Ogilvie, D., Finniear, R., Jenner, D., Powell, S., Anand, R., Smith, J. C., and Markham, A. F. A novel rapid method for the isolation of terminal sequences from yeast artificial chromosomes (YAC) clones.Nucleic Acids Res 18: 2887–2890, 1990

    Article  PubMed  CAS  Google Scholar 

  • Robey, F. A., Jones, K. D., Tanata, T., and Liu, T.-Y. Binding of C-reactive protein to chromatin and nucleosome core particles. A possible physiological role of C-reactive protein.J Biol Chem 259: 7311–7316, 1984

    PubMed  CAS  Google Scholar 

  • Rubio, N., Sharp, P. M., Rits, M., Zahedi, K., and Whitehead, A. S. Structure, expression and evolution of guinea pig serum amyloid P-component and C-reactive protein.J Biochem 113: 277–284, 1993

    PubMed  CAS  Google Scholar 

  • Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. Primer-directed enzyme amplification of DNA with a thermostable DNA polymerase.Science 239: 487–491, 1988

    Article  PubMed  CAS  Google Scholar 

  • Sammartino, L., Webber, L. M., Hogarth, P. M., McKenzie, I. F. C., and Garson, O. M. Assignment of the gene coding for human FcRII (CD32) to bands q23q24 on chromosome 1.Immunogenetics 28: 380–381, 1988

    Article  PubMed  CAS  Google Scholar 

  • Sanger, F., Niklen, S., and Coulson, A. R. DNA sequencing with chain termination inhibitors.Proc Natl Acad Sci USA 74: 5463–5467, 1977

    Article  PubMed  CAS  Google Scholar 

  • Schlimgen, A. K., Helms, J. A., Vogel, H., and Perin, M. S. Neuronal pentraxin, a secreted protein with homology to acute phase proteins of the immune system.Neuron 14: 519–526, 1995

    Article  PubMed  CAS  Google Scholar 

  • Seery, L. T., Schoenberg, D. R., Barbaux, S., Sharp, P. M., and Whitehead, A. S. Identification of a novel member of the pentraxin family inXenopus laevis.Proc R Soc Lond B 253: 263–270, 1993

    Article  CAS  Google Scholar 

  • Shephard, E. G., Smith, P. J., Coetzee, S., Strachan, A. F., and deBeer, F. C. Pentraxin binding to isolated rat liver nuclei.Biochemistry J 279: 257–260, 1991

    CAS  Google Scholar 

  • Siegel, J., Rent, R., and Gewurz, H. Interaction of the C-reactive protein with the complement system. I. Protamine-induced consumption of complement in acute phase sera.J Exp Med 140: 631–647, 1974

    Article  PubMed  CAS  Google Scholar 

  • Sierra, F., Stein, G., and Stein, J. Structure and in vitro transcription of a human H4 histone gene.Nucleic Acids Res. 11: 7069–7086, 1983

    Article  PubMed  CAS  Google Scholar 

  • Steel, D. M. and Whitehead, A. S. The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein.Immunol Today 15: 81–88, 1994

    Article  PubMed  CAS  Google Scholar 

  • Tay, A., Squire, A., Goldberg, H., and Skorecki, K. Assignment of the human prostaglandin-endoperoxide synthase 2 (PTGS2) gene to 1q25 by fluorescence in situ hybridisation.Genomics 23: 718–719, 1994

    Article  PubMed  CAS  Google Scholar 

  • Tennent, G. A., Lovat, L. B., and Pepys, M. B. Serum amyloid P component prevents proteolysis of the amyloid fibrils of Alzheimer's disease and systemic amyloidosis.Proc Nat Acad Sci USA 92: 4299–4303, 1995

    Article  PubMed  CAS  Google Scholar 

  • Tepler, I., Morton, C. C., Shimizu, A., Holcombe, R. F., Eddy, R., Shows, T. B., and Leder, P. The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localisation to 1q21-23 and RFLP analysis.Am J Hum Genet 45: 761–765, 1989

    PubMed  CAS  Google Scholar 

  • Trapani, J. A., Browne, K. A., Dawson, M. J., Ramsay, R. G., Eddy, R. L., Shows, T. B., White, P. C., and Dupont, B. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-γ in myeloid cells.Immunogenetics 36: 369–376, 1992

    Article  PubMed  CAS  Google Scholar 

  • Volz, A., Korge, B. P., Compton, J. G., Ziegler, A., Steinhert, P. M., and Mischke, D. Physical mapping of epidermal differentiation genes on chromosome 1q21.Genomics 18: 92–99, 1993

    Article  PubMed  CAS  Google Scholar 

  • Whitehead, A. S., Rits, M., and Michaelson, J. Molecular genetics of mouse serum amyloid P component (SAP): cloning and gene mapping.Immunogenetics 28: 388–390, 1988

    Article  PubMed  CAS  Google Scholar 

  • Woo, P., Korenberg, J. R., and Whitehead, A. S., Characterisation of genomic and complementary DNA sequence of human C-reactive protein, and comparison with the complementary DNA sequence of serum amyloid-P component.J Biol Chem 260: 13384–13388, 1985

    PubMed  CAS  Google Scholar 

  • Yuan, J., Wegenka, U. M., Lutticken, C., Buschmann, J., Decker, T., Schindler, C., Heinrich, P. C., and Horn, F. The signalling pathways of IL-6 and gamma interferon converge by the activation of different transcription factors which bind to common responsive DNA elements.Mol Cell Biol 14: 1657–1668, 1994

    PubMed  CAS  Google Scholar 

  • Yunis, I. and Whitehead, A. S. The mouse C-reactive protein gene maps to distal chromosome 1 and, like its human counterpart, is closely linked to the serum amyloid P-component gene.Immunogenetics 32: 361–363, 1990

    PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Aspasia Divane

    Present address: Diagnostic Genetic Centre, Kolokotroni 78, Piraeus, Greece

Authors and Affiliations

  1. Department of Genetics and Biotechnology Institute, University of Dublin, Trinity College, 2, Dublin, Ireland

    Marie-Therese Walsh & Alexander S. Whitehead

  2. Department of Pathology, University of Cambridge, Cambridge, England

    Aspasia Divane

Authors
  1. Marie-Therese Walsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aspasia Divane
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexander S. Whitehead
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Walsh, MT., Divane, A. & Whitehead, A.S. Fine mapping of the human pentraxin gene region on chromosome 1q23. Immunogenetics 44, 62–69 (1996). https://doi.org/10.1007/BF02602657

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02602657

Keywords

Search

Navigation