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Physical mapping of theE/C andgrc regions of the rat major histocompatibility complex

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Abstract

Alignment of class I-hybridizing cosmids from an R21 (A l B l D l E u grc +) genomic DNA library gave two contigs: one [150 kilobases (kb)] encompassed theE/C region, or a large part thereof, and the other (110 kb) contained thegrc region which has genes influencing resistance to chemical carcinogens (rcc), fertility (ft), and growth (dw-3). Amplification of gene sequences in the four cosmids in theE/C region usingE u-specific andLW2 (RT1.C)-specific primers showed that each cosmid contained bothE u-like andC-like genes. They are clearly different but closely associated, and they show some variation from the prototypicE (E u) andC (LW2) genes, respectively. Comparison of DNA fromgrc + andgrc − strains of rats showed that the deletion in thegrc − strains was approximately 50 kb, and that it was located on two of the three cosmids in thegrc-region contig. The use of specific class I probes showed that thegrc region contained tandemly duplicatedRT1.O-RT1.N genes and that theRT.BM1 loci lay outside of thegrc region. Neither contig reacted with probes specific for class II,TNFA, Hsp70, orRT1.M genes. The data presented here and the previous data in the literature (summarized in Gill et al. 1995) suggest that the gene order in the major histocompatibility complex (MHC) andMHC-linked region of the rat is:A-E/C-grc-M.

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  1. Andrea L. Cortese Hassett

    Present address: Cytology Laboratory, Central Blood Bank, 3636 Boulevard of the Allies, 15213, Pittsburgh, PA, USA

Authors and Affiliations

  1. Department of Pathology, University of Pittsburgh, School of Medicine, 15261, Pittsburgh, PA, USA

    Xiu-Juan Yuan, Shashikumar K. Salgar, Andrea L. Cortese Hassett, Kathleen P. McHugh, Heinz W. Kunz & Thomas J. Gill III

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  1. Xiu-Juan Yuan
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  2. Shashikumar K. Salgar
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Yuan, XJ., Salgar, S.K., Cortese Hassett, A.L. et al. Physical mapping of theE/C andgrc regions of the rat major histocompatibility complex. Immunogenetics 44, 9–18 (1996). https://doi.org/10.1007/BF02602652

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