Skip to main content
SpringerLink
Log in

Substrate specificity of carboxyl proteinase ofAspergillus sojae

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The specificity and mode of action ofAspergillus sojae carboxyl proteinase I were investigated with the oxidized B-chain of insulin.A. sojae carboxyl proteinase I hydrolyzed primarily two peptide bonds in the oxidized B-chain of insulin, the Leu15-Tyr16 bond and the Phe24-Phe25 bond. Additional cleavage of the bond Tyr16-Leu17 was also noted.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Craig, L. C., Konigsberg, W., King, T. P. 1961. Peptide chains (A and B) from beef insulin. Biochemical Preparations8:70–75.

    CAS  Google Scholar 

  2. Frankel-Conrat, H., Harris, J. I., Levy, A. L. 1955. Recent developments in techniques for terminal and sequence studies in peptides and proteins, pp. 359–425. In: Glick, D. (ed.), Methods in biochemical analysis, vol. II. New York: Interscience.

    Chapter  Google Scholar 

  3. Gabriel, O. 1971. Analytical disc gel electrophoresis, pp. 565–578. In: Jakoby, W. B. (ed.) Methods in enzymology, vol. XXII. New York: Academic Press.

    Google Scholar 

  4. Griffin, T. B., Wagner, F. W., Prescott, J. M. 1966. Anion exchange chromatography of oxidized insulin peptides. Journal of Chromatography23:280–286.

    Article  PubMed  CAS  Google Scholar 

  5. Hayashi, K., Mizunuma, T. 1974. Acid proteinase from soy sauce brewing fungusAspergillus sojae, p. 42. In: Abstract Papers of Agricultural Chemical Society of Japan. Tokyo: Agricultural Chemical Society of Japan.

    Google Scholar 

  6. Ichihara, S., Uchino, F. 1975. The specificity of acid proteinase fromAcrocylindrium. Agricultural Biological Chemistry39:423–428.

    CAS  Google Scholar 

  7. Ichishima, E. 1970. Purification and mode of assay for acid proteinase ofAspergillus saitoi, pp. 397–406. In: Perlmann, G. E., Lorand, L. (eds.), Methods in enzymology New York: Academic Press.

    Google Scholar 

  8. Ichishima, E. 1972. Purification and characterization of a new type of acid carboxypeptidase fromAspergillus saitoi. Biochimica et Biophysica Acta293:444–450.

    Google Scholar 

  9. IUPAC and IUB Recommendations. 1972. Units of enzyme activity, pp. 26–27. In: Comprehensive biochemistry. Enzyme nomenclature, vol. 13. Amsterdam: Elsevier

    Google Scholar 

  10. Mains, G., Takahashi, M., Sodek, J., Hofmann, T. 1971. The specificity of penicillopepsin. Canadian Journal of Biochemistry49:1134–1149.

    Article  PubMed  CAS  Google Scholar 

  11. Oda, K., Murao, S. 1976. Action ofScytalidium lignicolumn acid proteinases on insulin B-chain. Agricultural Biological Chemistry40:1221–1225.

    CAS  Google Scholar 

  12. Reichelt, D., Jacobson, E., Haschen, R. T. 1974. Purification and properties of cathepsin D from human erythrocytes. Biochimica et Biophysica Acta341:15–26.

    PubMed  CAS  Google Scholar 

  13. Roberts, N. B., Taylor, W. H. 1978. The isolation and properties of a non-pepsin proteinase from human gastric mucosa. Biochemical Journal169:617–624.

    PubMed  CAS  Google Scholar 

  14. Roberts, N. B., Taylor, W. H. 1979. Action of human pepsins 1, 2, 3 and 5 on the oxidized B-chain of insulin. Biochemical Journal179:183–190.

    PubMed  CAS  Google Scholar 

  15. Ryle, A. P., Lecherc, J., Falla, F. 1968. The substrate specificity of pepsin C. Biochemical Journal110:4p.

  16. Sanger, F., Tuppy, H. 1951. The amino acid sequence in the phenylalanyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates. Biochemical Journal49:481–490.

    PubMed  CAS  Google Scholar 

  17. Tanaka, N., Takeuchi, M., Ichishima, E. 1977. Purification of an acid proteinase fromAspergillus saitoi and determination of peptide bond specificity. Biochimica et Biophysica Acta485:406–416.

    PubMed  CAS  Google Scholar 

  18. Tsuru, D., Hattori, A., Tsuji, H., Yamamoto, T., Fukumoto, J. 1969. Studies on mold proteases. Part II. Substrate specificity of acid protease ofRhizopus chinensis. Agricultural Biological Chemistry33:1419–1426.

    CAS  Google Scholar 

  19. Vesterberg, O. 1971. Isoelectric focusing of proteins, pp. 389–412. In: Jakoby, W. B. (ed.), Methods in enzymology, vol. XXII. New York: Academic Press.

    Google Scholar 

  20. Yamamoto, K., Hayashi, K. 1961. Proteinase-inhibitor from the cell free extract ofAspergillus sojae and its inactivation. Symposium of Enzyme Chemistry, Japan13:137–139.

    Google Scholar 

  21. Yong, F. M., Wood, B. J. B. 1974. Microbiology and biochemistry of soy sauce fermentation, pp. 157–194. In: Perlman, D. (ed.), Advances in applied microbiology, vol. 17. New York: Academic Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Enzymology and Microbial Chemistry, Tokyo Noko University, Fuchu, 183, Tokyo, Japan

    Takeshi Kimura, Yasuhiro Mayumi, Michio Takeuchi & Eiji Ichishima

  2. Central Research Laboratories of Kikkoman Shoyu Co., Noda, Chiba, Japan

    Kazuya Hayashi

Authors
  1. Takeshi Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuhiro Mayumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michio Takeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazuya Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eiji Ichishima
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kimura, T., Mayumi, Y., Takeuchi, M. et al. Substrate specificity of carboxyl proteinase ofAspergillus sojae . Current Microbiology 3, 153–156 (1979). https://doi.org/10.1007/BF02601858

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02601858

Keywords

Search

Navigation