Skip to main content
SpringerLink
Log in

Mechanism of the effect of levorin on transport of amino acids inCandida albicans

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

By use of incubation media of various ionic compositions and pH's, it was possible to reveal that inhibition of the transport of neutral amino acids having long hydrophobic chains (leucine, phenylalanine) inCandida albicans by the polyene antibiotic levorin was mainly due to redistribution—caused by the antibiotic—of monovalent cations in the cells and protoplasts of the yeast. In an acidic medium, levorin was also found to induce a decrease of proton release from the cells, accompanied by some inhibition of leucine transport. Transport of neutral amino acids having short hydrophobic chains (alanine, glycine, proline) was inhibited by this polyene antibiotic, irrespective of the ionic composition or pH of the medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Bradley, S. G., Jones, L. A. 1960. Mechanisms of action of antibiotics. Annals of the New York Academy of Sciences89:122–133.

    Article  CAS  Google Scholar 

  2. Johnson, B., White, R. J., Williamson, G. M. 1978. Factors influencing the susceptibility ofCandida albicans to the polyenoic antibiotics nystatin and amphotericin B. Journal of General Microbiology104:325–333.

    PubMed  CAS  Google Scholar 

  3. Karst, F., Jund, R. 1976. Sterol replacement inSaccharomyces cerevisiae. Effect on cellular permeability and sensitivity to nystatin. Biochemical and Biophysical Research Communications71:535–543.

    Article  PubMed  CAS  Google Scholar 

  4. Kotyk, A. 1976. Transport in yeast, the simplest eucaryotic organisms, Third Winter School in Biophysics of Membrane Transport. Poland. Biophysics of Membrane Transport. Part 1, 9–39.

    Google Scholar 

  5. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry193:265–275.

    PubMed  CAS  Google Scholar 

  6. Obukhovskaya, A. S., Lishnevskaya, E. B., Tereshin, I. M. 1978. Dependence of the inhibition by levorin of the amino acid incorporation into protoplasts and subcellular proteins ofCandida albicans on the change of potassium endocellular concentration [In Russian, with English summary]. Biokhimia43:127–131.

    CAS  Google Scholar 

  7. Saiyid, N. H., Kotyk, A. 1972. Amino acid transport inRhodotorula glutinis. Folia Microbiologia17:261–268.

    Article  CAS  Google Scholar 

  8. Schwencke, J., Magana-Schwencke, N. 1969. Depression of proline transport system inSaccharomyces chevalieri by nitrogen starvation. Biochemica et Biophysica Acta173:302–312.

    Article  CAS  Google Scholar 

  9. Termkhitarova, N. G., Shulga, A. V. 1974. Determination of protein in yeast cells [In Russian, with English summary]. Prikladnaya Biokhimia i Mikrobiologiya8:928–932.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The USSR Research Technological Institute of Antibiotics and Enzymes for Medical Use, Ogorodnikov Prospekt, 41, 198020, Leningrad, USSR

    A. S. Obukhovskaya, E. B. Lishnevskaya & I. M. Tereshin

Authors
  1. A. S. Obukhovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. B. Lishnevskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. M. Tereshin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Obukhovskaya, A.S., Lishnevskaya, E.B. & Tereshin, I.M. Mechanism of the effect of levorin on transport of amino acids inCandida albicans . Current Microbiology 2, 219–222 (1979). https://doi.org/10.1007/BF02601716

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02601716

Keywords

Search

Navigation