Skip to main content
SpringerLink
Log in

Composition of membranes and mycelia ofStreptomyces levoris at different stages of growth

  • Published:
Current Microbiology Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

The composition of cell membranes and mycelia ofStreptomyces levoris, producer of the polyene antibiotic levorin, was studied. The membrane protein/lipid ratio was shown to be constant during growth of the microorganism. The membrane protein was found to be heterogeneous and to have a low molecular weight. The lipid component of the membranes consisted mainly of polar lipids—phosphatidyl ethanolamine, diphosphatidyl glycerol, and phosphatidyl inositol mannoside being predominant. During growth, the phospholipid content of the polar lipid fraction decreased, apparently due to replacement of phospholipids by phosphorus-free analogs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Batrakov, S. G., Pridachina, N. N., Kruglyak, E. B., Martyakova, A. V., Bergelson, L. D. 1977. The ornithine containing lipids fromActinomyces aureoverticillus, the possible exchangeability of phosphatidylethanolamine and ornithinolipids in the cell membranes of actinomycetes. [In Russian, with English summary]. Bioorganicheskaya Khimiya3:920–929.

    CAS  Google Scholar 

  2. Chen, P. S., Toribara, T. Y., Warner, H. 1956. Microdetermination of phosphorus. Analytical Chemistry28:1756–1758.

    Article  CAS  Google Scholar 

  3. Diche, Z., Schwarz, K. 1937. Mikromethode zur Bestimmung verschiedener Pentosen nebeneinander bei Gegenwart von Hexosen. Mikrochimica Acta2:13–19.

    Article  Google Scholar 

  4. Dorokhova, L. A., Agre, N. S., Krasil'nikov, N. A. 1971. Ultrastructure of hyphae and spores inActinomyces megasporus. [In Russian, with English summary]. Mikrobiologiya40:517–521.

    CAS  Google Scholar 

  5. Efimova, T. P., Pechatnikova, I. Sh., Tereshin, I. M. 1977. The composition of membranes ofActinomyces hygroscopicus during course of growth and development. [In Russian, with English summary]. Mikrobiologiya46:676–682.

    CAS  Google Scholar 

  6. Efimova, T. P., Pechatnikova, I. Sh., Tereshin, I. M. 1978. Composition of membranes ofActinomyces hygroscopicus. [In Russian, with English Summary]. Mikrobiologiya47:85–90.

    CAS  Google Scholar 

  7. Huber, M. L. B., Godfrey, O. 1978. A general method for lysis ofStreptomyces species. Canadian Journal of Microbiology24:631–632.

    Article  PubMed  CAS  Google Scholar 

  8. Kates, M. 1972. Techniques of lipidology. Isolation analysis and identification of lipids. Amsterdam, London: North Holland Publishing Co.

    Google Scholar 

  9. Keller, U., Kleinkauf, H. 1977. Studies of the biosynthesis of actinomycin in protoplasts fromStreptomyces antibioticus. Archives of Biochemistry and Biophysics184:111–124.

    Article  PubMed  CAS  Google Scholar 

  10. Kulalaeva, Z. I., Poltorak, V. A., Zaichkin, E. I., Tiurin, V. S., Silaev, A. B. 1976. Vegetative mycelium ofActinomyces flavus studied by electron microscopy in the course of biosynthesis of flavofungin. [In Russian, with English summary]. Mikrobiologiya45:133–136.

    CAS  Google Scholar 

  11. Kurylowicz, W. 1977. The site of antibiotic accumulation in streptomycetes andPenicillium chrysogenum. Acta Microbiologica Academiae Scientiarum Hungaricae24:263–271.

    CAS  Google Scholar 

  12. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry193:265–275.

    PubMed  CAS  Google Scholar 

  13. Lugtenberg, B., Meijers, J., Peters, R., van der Hoek, P., van Alphen, L. 1975. Electrophoretic resolution of the major outer membrane protein ofEscherichia coli K-12 into four bands. FEBS Letters58:254–258.

    Article  PubMed  CAS  Google Scholar 

  14. Minnikin, D. F., Abdolrahimzaden, H. 1974. The replacement of phosphatidylethanolamine and acidic phospholipids by an ornithineamine lipid and minor phosphorus-free lipid inPseudomonas fluorescens NCMB 129. FEBS Letters43:257–260.

    Article  PubMed  CAS  Google Scholar 

  15. Muñoz, E., Marquet, A., Larraga, V., Coyette, J. 1972. Isolation, partial characterization of the cytoplasmic membrane fraction ofStreptomyces albus G andDD-carboxypeptidase localization. Archiv für Mikrobiologie81:273–288.

    Article  PubMed  Google Scholar 

  16. Okanishi, M. R., Suzuki, K., Umezawa, H. 1974. Formation and reversion of streptomycete protoplasts: Cultural condition and morphological study. Journal of General Microbiology80:389–400.

    PubMed  CAS  Google Scholar 

  17. Pechatnikova, I. Sh., Efimova, T. P., Tereshin, I. M. 1976. Obtainment of protoplasts and membranes fromActinomyces hygroscopicus. [In Russian, with English summary]. Mikrobiologiya45:859–863.

    CAS  Google Scholar 

  18. Salton, M. R. J., Freer, J. H. 1965. Composition of the membranes isolated from several Gram-positive bacteria. Biochimica et Biophysica Acta107:531–538.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The USSR Research Technological Institute of Antibiotics and Enzymes for Medical Use, Ogorodnikov Prospekt 41, 198020, Leningrad, USSR

    M. L. Zuzina, T. P. Efimova & I. M. Tereshin

Authors
  1. M. L. Zuzina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. P. Efimova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. M. Tereshin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zuzina, M.L., Efimova, T.P. & Tereshin, I.M. Composition of membranes and mycelia ofStreptomyces levoris at different stages of growth. Current Microbiology 2, 215–217 (1979). https://doi.org/10.1007/BF02601715

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02601715

Keywords

Search

Navigation