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A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA

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Abstract

Bleomycin treatment of PM2 DNA results in fragmentation of the genome at several specific sites. Application of restriction endonuclease digestion followed by bleomycin treatment has provided the basis for constructing a physical map of bleomycin fragmentation sites. Eleven sites have been located on the physical map relative toHpa II,Pst I, andHindIII cleavage sites. The fragmentation sites are not clustered in a particular region of the PM2 genome but 3 of the 11 sites do occur between theHpa II andPst I cleavage sites, a segment of DNA which comprises 14% of the PM2 DNA length.

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Literature Cited

  1. Brack, C., Eberle, H., Bickle, T. A., Yuan, R. 1976. A map of the sites on bacteriophage PM2 DNA for the restriction endonucleasesHindIII andHpaII. Journal of Molecular Biology104:305–309.

    Article  PubMed  CAS  Google Scholar 

  2. Crooke, S. S., Bradner, W. T. 1976. Bleomycin, a review. Journal of Medicine7:333–428.

    PubMed  CAS  Google Scholar 

  3. Davis, R. W., Simon, M., Davidson, N. 1971. Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids, pp. 413–428. In: Grossman, L., Moldave, K. (eds.) Methods in enzymology. New York: Academic Press.

    Google Scholar 

  4. Espejo, R. T., Canelo, E. S. 1968. Properties of bacteriophage PM2: A lipid-containing bacterial virus. Virology34:738–747.

    Article  PubMed  CAS  Google Scholar 

  5. Freifelder, D. 1969. Matching of single-strand breaks to form double-strand breaks in DNA. Biopolymers7:681–693.

    Article  CAS  Google Scholar 

  6. Haidle, C. W. 1971. Fragmentation of deoxyribonucleic acid by bleomycin. Molecular Pharmacology7:645–652.

    PubMed  CAS  Google Scholar 

  7. Haidle, C. W., Weiss, K. K., Kuo, M. T. 1972. Release of free bases from deoxyribonucleic acid after reaction, with bleomycin. Molecular Pharmacology8:531–537.

    PubMed  CAS  Google Scholar 

  8. Hsu, M., Berg, P. 1978. Altering the specificity of restriction endonuclease: Effect of replacing Mg2+ with Mn2+. Biochemistry17:131–138.

    Article  PubMed  CAS  Google Scholar 

  9. Lloyd, R. S., Haidle, C. W., Robberson, D. L. 1978. Bleomycin-specific fragmentation of double-stranded DNA. Biochemistry, in press.

  10. Lloyd, R. S., Hewitt, R. R., Haidle, C. W. 1977. Bleomycin-induced alkaline-labile damage and direct strand breakage in covalent-circular DNA. Federation Proceedings36:694.

    Google Scholar 

  11. McDonell, M. W., Simon, M. N., Studier, F. W. 1977. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology110:119–146.

    Article  PubMed  CAS  Google Scholar 

  12. Müller, W. E. G., Yamazaki, Z., Zahn, R. K. 1972. Action of bleomycin on DNA and RNA. European Journal of Biochemistry31:518–525.

    Article  PubMed  Google Scholar 

  13. Parker, R. C., Watson, R. M., Vinograd, J. 1977. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis. Proceedings of the National Academy of Sciences74:851–855.

    Article  CAS  Google Scholar 

  14. Povirk, L. F., Wübker, W. Köhnlein, W., Hutchinson, F. 1977. DNA double-strand breaks and alkali-labile bonds produced by bleomycin. Nucleic Acids Research4:3573–3580.

    Article  PubMed  CAS  Google Scholar 

  15. Salditt, M., Braunstein, S. N., Camerini-Otero, R. D., Franklin, R. M. 1972. Structure and synthesis of a lipid-containing bacteriophage. Virology48:259–262.

    Article  PubMed  CAS  Google Scholar 

  16. Strong, J. E., Hewitt, R. R. 1975. Investigation of human DNases by DNA-polyacrylamide gel electrophoresis, pp. 473–483. In: Markert, C. (ed.), Isozymes, vol. 3: Developmental biology. New York: Academic Press.

    Google Scholar 

  17. Sugden, B., DeTroy, B., Roberts, R. J., Sambrook, J. 1975. Agarose slab-gel electrophoresis equipment. Analytic Biochemistry68:36–46.

    Article  CAS  Google Scholar 

  18. Suzuki, H., Nagai, K., Yamaki, H., Tanaka, N., Umezawa, H. 1969. On the mechanism of action of bleomycin: Scission of DNA strandsin vitro andin vivo. Journal of Antibiotics22:446–448.

    PubMed  CAS  Google Scholar 

  19. Thomas, M., Davis, R. W. 1975. Studies on the cleavage of bacteriophage lamda DNA withEcoRI restriction endonuclease. Journal of Molecular Biology91:315–328.

    Article  PubMed  CAS  Google Scholar 

  20. Umezawa, H., Maeda, K., Takeuchi, T., Oakami, Y. 1966. New antibiotics, bleomycin A and B. Journal of Antibiotics19:200–209.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 77030, Houston, Texas, USA

    R. Stephen Lloyd, Charles W. Haidle & Donald L. Robberson

  2. Environmental Science Park, Research Division, The University of Texas System Cancer Center, 78957, Smithville, Texas, USA

    Marion L. Dodson Jr.

Authors
  1. R. Stephen Lloyd
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  2. Charles W. Haidle
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  3. Donald L. Robberson
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  4. Marion L. Dodson Jr.
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Lloyd, R.S., Haidle, C.W., Robberson, D.L. et al. A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA. Current Microbiology 1, 45–50 (1978). https://doi.org/10.1007/BF02601707

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