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Quantitative complement-fixation

II. Standardization of the unit of complement

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Antonie van Leeuwenhoek Aims and scope Submit manuscript

Summary

Using as a diluent a veronal buffer with Mg and Ca ions a wide range of concentrations of hemolytic antibody, prepared according to the prescription of the New York State Department of Health, could sensitize different lots of Alsever preserved sheep red cells to the same maximal degree.

The 50% (Ko) unit of complement, chemically preserved according toRichardson (1941), remains fairly constant during a period of two weeks against maximally sensitized red cells. At the end of this period the susceptibility of the sensitized cells may suddenly increase with about 10%. The moment of this change in susceptibility unfortunately cannot be predicted.

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Author notes

  1. J. H. de Boer

    Present address: Mathematical Institute, State University of Groningen, Groningen, the Netherlands

Authors and Affiliations

  1. Laboratory for Bacteriology and Serology, State University, Groningen

    H. H. Cohen, B. Van Linge, J. H. de Boer &  H. Polman

Authors
  1. H. H. Cohen
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  2. B. Van Linge
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  3. J. H. de Boer
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  4. H. Polman
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Cohen, H.H., Van Linge, B., de Boer, J.H. et al. Quantitative complement-fixation. Antonie van Leeuwenhoek 19, 197–210 (1953). https://doi.org/10.1007/BF02594847

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  • DOI: https://doi.org/10.1007/BF02594847

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