Dose and scanning delay using USPIO for central nervous system macrophage imaging

  • Vincent Dousset
  • Christophe Gomez
  • Klaus G. Petry
  • Christophe Delalande
  • Jean-Marie Caille
Article

Abstract

Rationale and objectives: In experimental allergic encephalomyelitis (EAE), central nervous system (CNS) macrophage imaging is achievable by MRI using AMI-227 an ultra-small particle iron oxide contrast agent at a dose of 300 μmol/kg Fe. The objective was to test the feasibility at the human recommended dose of 45 μmol/kg Fe.Methods: Two groups of EAE rats were tested with AMI-227 using 45 and 300 μmol/kg Fe respectively. Following i.v. injection of AMI-227, they were scanned after a delay of 4–6 and 20–24 h.Results: With a high dose of AMI-227, all animals showed low signal intensity related to iron-loaded macrophages in the CNS. At low dose no abnormalities were found in the CNS. Furthermore, a delay of 4–6 h failed to demonstrate abnormalities even at high dose.Conclusions: Dose, scanning delay after administration and blood half-life are major parameters for T2* CNS macrophage imaging.

Keywords

MRI Allergic encephalomyelitis Macrophages Contrast agents 

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Copyright information

© Elsevier Science B.V. All rights reserved 1999

Authors and Affiliations

  • Vincent Dousset
    • 1
  • Christophe Gomez
    • 1
  • Klaus G. Petry
    • 2
  • Christophe Delalande
    • 3
  • Jean-Marie Caille
    • 1
  1. 1.Laboratoire de Neurobiologie et Neuroimagerie ExpérimentalesUniversité Victor Segalen Bordeaux 2BordeauxFrance
  2. 2.Laboratoire de Neurobiologie IntégrativeINSERM 394BordeauxFrance
  3. 3.Laboratoire de Résonance Magnétique des Systèmes BiologiquesCNRS/Université Victor Segalen Bordeaux 2BordeauxFrance

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