Lasers in Medical Science

, Volume 3, Issue 1–4, pp 99–102 | Cite as

Detection of near-infrared porphyrin fluorescence excited in experimental animal tumour by the HeNe laser

  • M. Zandomeneghi
  • C. Festa
  • C. A. Angelletti
  • G. Menconi
  • T. Sicuro
  • I. Cozzani


The red emission (632 nm) of a low-power (about 10 mW) HeNe laser is used to excite liposome-bound haematoporphyrin dimethylester localized in a solid tumour implanted in the mouse leg (MS-2 sarcoma). Fluorescence from the target area, filtered at >710 nm, is converted into visible green light by means of a near-infrared-visible image converter. The photographically recorded images clearly reveal differences in fluorescence intensity of tumour tissues with respect to normal tissues. Clinical application of the procedure is proposed, and the expected advantage with respect to current fluorescence endoscopy are discussed.


Haematoporphyrin Red light laser Fluorescence endoscopy Tumour detection 


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  1. 1.
    Policard A. Etude sur les aspects offerts par des tumeurs expérimentales examinées à la lumière de Wood.CR Soc Biol 1924,91:1423–4Google Scholar
  2. 2.
    Auler H, Banzer G. Untersuchungen über die Rolle der Porphyrine geschwulstkranken Menschen und Tieren.Z Krebsforsch 1943,53:65–8Google Scholar
  3. 3.
    Lipson RL, Baldes EJ, Olsen AM. The use of a derivative of hematoporphyrin in tumor detection.J Natl Cancer Inst 1961,26:1–11PubMedGoogle Scholar
  4. 4.
    Lipson RL, Baldes EJ, Gray MJ. Hematoporphyrin derivative for detection and management of cancer.Cancer 1967,20:2255–7PubMedCrossRefGoogle Scholar
  5. 5.
    Profio AE, Doiron DR, King EG. Laser fluorescence bronchoscopy for localization of occult lung tumors.Med Phys 1979,6:523–5PubMedCrossRefGoogle Scholar
  6. 6.
    Cortese DA, Kinsey JH, Woolner RB et al. Clinical applications of a new endoscopic technique for detection of in situ bronchial carcinoma.Mayo Clin Proc 1979,34: 635–41Google Scholar
  7. 7.
    Profio AE, Doiron DR, Balchum OJ et al. Fluorescence bronchoscopy for localization of carcinoma in situ.Med Phys 1983,10:35–9PubMedCrossRefGoogle Scholar
  8. 8.
    Zandomeneghi M, Festa C, Cozzani I. Detection of near-infrared porphyrin fluorescence excited in vivo by low-power red laser. 1st European Congress on Photobiology, Grenoble, 1986:236 (abstr.)Google Scholar
  9. 9.
    Gijsbers GHM, Breederrel D, van Gemert MJC et al. In vivo fluorescence excitation and emission spectra of hematoporphyrin-derivative.Laser Life Sci 1986,1:29–48Google Scholar
  10. 10.
    Jori G, Cozzani I, Reddi E et al. In vitro and in vivo studies on the interaction of hematoporphyrin and its dimethylester with normal and malignant cells. In: Doiron DR, Gomer CF (eds)Porphyrin localization and treatment of tumors. New York: AR Liss, 1984:471–82Google Scholar
  11. 11.
    Cozzani I, Jori G, Reddi E et al. Interaction of free and liposome-bound porphyrins with normal and malignant cells: biochemical and photosensitization studies in vitro and in vivo. In: Andreoni A, Cubeddu R (eds)Porphyrins in tumor phototherapy. New York: Plenum Press, 1984:157–65Google Scholar
  12. 12.
    Sicuro T, Carlini P, Scarcelli, V, et al. Mechanisms of porphyrin binding and photosensitization at the subcellular and molecular levels in normal and malignant cells and tissues. In: ASI Series textbookPrimary photoprocesses in biology and medicine. New York: Plenum Press, 1984:367–9Google Scholar
  13. 13.
    Jori G, Beltramini M, Reddi E et al. Evidence for a major role of plasma lipoproteins as hematoporphyrine carriers in vivo.Cancer Lett 1984:291–7Google Scholar
  14. 14.
    Jori G, Reddi E, Cozzani I et al. Controlled targeting of subcellular sites by porphyrins in tumor bearing mice.Br J Cancer 1986,53:615–21PubMedGoogle Scholar
  15. 15.
    Cozzani I, Vigna MF, Zandomeneghi M et al. Biochemical and biophysical factors controlling porphyrin-sensitized photodynamic action in isolated cells and in spontaneous tumor. In: Jori G, Perria C (eds)Photodynamic therary of tumors and other diseases. Padova: Libreria Progetto, 1985:149–52Google Scholar
  16. 16.
    Ben-Hur E, Rosenthal I. Photosensitization of Chinese hamster cells by water-soluble phthalocyanines.Photochem Photobiol 1986,43:615–9PubMedGoogle Scholar
  17. 17.
    Spikes JD. Phthalocyanines as photosensitizers in biological systems and for the photodynamic therapy of tumors.Photochem Photobiol 1986,43:615–9Google Scholar
  18. 18.
    Chan WS, Hart IR. Cellular studies on the uptake and retention of hematoporphyrin derivative and aluminium sulphonated phthalocyanine. In: Jori G, Perria C (eds)Photodynamic therapy of tumors and other diseases. Padova: Libreria Progetto, 1985:155–8Google Scholar

Copyright information

© Baillière Tindall 1988

Authors and Affiliations

  • M. Zandomeneghi
    • 1
  • C. Festa
    • 1
  • C. A. Angelletti
    • 2
  • G. Menconi
    • 2
  • T. Sicuro
    • 3
  • I. Cozzani
    • 3
  1. 1.Dipartimento di Chimica e Chimica Industriale dell'Università di PisaPisaItaly
  2. 2.Clinical Chirurgical Generale dell'Università di PisaItaly
  3. 3.Cattedra di Biochimica ApplicataFacoltà di Medicina e Chirurgia dell'Università di Reggio CalabriaItaly

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