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Lasers in Medical Science

, Volume 3, Issue 1–4, pp 71–80 | Cite as

Chromatographic analysis and tissue distribution of radiocopper-labelled haematoporphyrin derivatives

  • Brian C. Wilson
  • Gunter Firnau
  • W. Patrick Jeeves
  • Kay L. Brown
  • Diane M. Burns-McCormick
Article

Abstract

The photosensitizers haematoporphyrin derivative (HPD) and dihaematoporphyrin ether/ester (DHE) have been labelled with the radionuclide copper-64 to allow non-invasive measurement of the concentration of photosensitizer in tumour and normal tissues. The effects of labelling have been assessed by gel and high-performance liquid chromatography.

Direct comparison of the tissue uptake and clearance of64Cu-labelled HPD/DHE with those of [14C]HPD/DHE has been made in mice bearing intramuscular radiation-induced fibrosarcoma (RIF) tumours, by gamma and beta counting of tumour, skin, liver, muscle, lung, kidney, brain, and blood samples at 1–72 h after intraperitoneal injection of test and control drug. The uptake and clearance curves of64Cu-labelled HPD or DHE in tumour, liver and blood agree to within 20% with those for the14C-labelled control.

Non-invasive quantification of64Cu-labelled DHE by gamma counting in vivo in rabbits bearing VX2 tumours in the ear shows good agreement with the photosensitizer concentrations in tumour and liver measured post-mortem in tissue samples.

Key words

Photodynamic therapy Haematoporphyrin derivative Chromatography Biodistribution Radiolabelling 

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Copyright information

© Baillière Tindall 1988

Authors and Affiliations

  • Brian C. Wilson
    • 1
  • Gunter Firnau
    • 1
  • W. Patrick Jeeves
    • 1
  • Kay L. Brown
    • 1
  • Diane M. Burns-McCormick
    • 1
  1. 1.Hamilton Regional Cancer Centre and McMaster UniversityHamiltonCanada

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