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Luminol-enhanced, whole blood chemiluminescence of human neutrophils evaluated by means of an automated, computer-assisted, and high-sensitivity luminescence analyzer

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International Journal of Clinical and Laboratory Research

Summary

Luminol-enhanced whole blood chemiluminescence of human neutrophils was studied using opsonized zymosan as a stimulus. Heparinized blood (0.5μl) was used, and the chemiluminescence signals were recorded by a very sensitive, automated, and computer-assisted luminometer (LB 950, Berthold, Wildbad, Germany). The following parameters were provided: integral values over the total measuring time, peak values, the time to reach maximum value, and the time to reach half maximum value. Normal subjects, neutropenic patients, subjects with total or partial myeloperoxidase deficiency, patients with recurrent infections, phagocytic defects, thrombocythemic patients, and those with non-Hodgkin's lymphomas undergoing therapy with recombinant human granulocyte colony-stimulating factor were studied. The integral response of chemiluminescence and the time of reach half maximal value were useful indicators of chemiluminescence defects; the assay, was able to detect chemiluminescence responses in neutropenic subjects with neutrophil levels as low as 0.6×109/I; differences between cellular and plasma defects could be identified; the quenching effect of exerted by erythrocytes was negligible.

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Authors and Affiliations

  1. Hematology Unit, Medical Clinic 1, University of Pisa, Ospedale S. Chiara, Via Roma 67, I-56100, Pisa, Italy

    G. Carulli, S. Minnucci, C. Angiolini, A. Azzarà & F. Ambrogi

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  1. G. Carulli
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  2. S. Minnucci
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  3. C. Angiolini
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  4. A. Azzarà
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  5. F. Ambrogi
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Carulli, G., Minnucci, S., Angiolini, C. et al. Luminol-enhanced, whole blood chemiluminescence of human neutrophils evaluated by means of an automated, computer-assisted, and high-sensitivity luminescence analyzer. Int J Clin Lab Res 25, 216–221 (1995). https://doi.org/10.1007/BF02592701

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  • DOI: https://doi.org/10.1007/BF02592701

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