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Comparison of different methods for the detection of autoantibodies in autoimmune diseases

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International Journal of Clinical and Laboratory Research

An Erratum to this article was published on 01 March 1996

Summary

Different immunological techniques were compared for their sensitivity in detecting some important autoantibodies in the sera of patients with rheumatic diseases. Sera of patients with systemic lupus erythematosus were screened for anti-dsDNA, Sm, and RNP autoantibodies byCrithidia luciliae assay, Farr assay, enzyme immunoassay, and immunoblotting. Sera of patients with Sjögren's syndrome were screened for anti-SSA and anti-SSB antibodies by enzyme immunoassay, counter immunoelectrophoresis, and immunoblotting and sera of patients with scleroderma for SCL-70 autoantibodies by enzyme immunoassay counter immunoelectrophoresis, and immunofluorescence on Hep-2 cells. Enzyme immunoassay and counter immunoelectrophoresis gave the most positive results and the best agreement compared with the other techniques. Immunofluorescence gave few positive results for each antibody evaluated. Immunoblotting gave intermediate results for all autoantibodies except anti-SSA, where the prevalence was low. There was no relationship between levels of dsDNA, Sm, and RNP antibodies and disease activity score in systemic lupus erythematosus patients.

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Authors and Affiliations

  1. Laboratorip di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 1-40136, Bologna, Italy

    B. Grigolo, I. Mazzetti, R. M. Borzì & A. Facchini

  2. Patologia Medica I, Università degli Studi di Bologna, Bologna, Italy

    R. Meliconi

  3. Istituto di Medicina Interna, Università degli Studi di Milano, Milano, Italy

    L. Origgi & R. Scorza

  4. Istituto di Clinica Medica e Gastroenterologia, Università degli Studi di Bologna, Bologna, Italy

    A. Facchini

Authors
  1. B. Grigolo
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  2. I. Mazzetti
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  3. R. M. Borzì
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  4. R. Meliconi
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  5. L. Origgi
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  6. R. Scorza
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  7. A. Facchini
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02644774.

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Grigolo, B., Mazzetti, I., Borzì, R.M. et al. Comparison of different methods for the detection of autoantibodies in autoimmune diseases. Int J Clin Lab Res 25, 205–210 (1995). https://doi.org/10.1007/BF02592699

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  • DOI: https://doi.org/10.1007/BF02592699

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