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Circulating levels of soluble CD23 reflect clinical and biological features of leukemic B-cell chronic lymphoproliferative disorders

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International Journal of Clinical and Laboratory Research

Summary

One hundred and twenty-four sera, from patients with various leukemic B-cell chronic lymphoproliferative diseases were investigated at diagnosis by ELISA for their soluble CD23 content. Immunophenotyping was carried out in all patients, and in a selected subset the mean number of membrane-bound CD23 molecules per cell was also investigated. Seventy-three patients had typical B chronic lymphocytic leukenia, 41 leukemic B-cell disorders with atypical morphological and/or immunophenotypic features, 5 had low-grade follicular cell lymphoma in the leukemic phase, and 5 had splenic lymphoma with villous lymphocytes Soluble CD23 levels were significantly higher than in normal sera (mean±SD: typical B chronic lymphocytic leukemia 3,650±4,654 U/ml, atypical B chronic lymphocytic leukemia 3,440±4,671 U/ml, follicular cell lymphoma 3,200±1,511 U/ml, splenic lymphoma with villous lymphocytes 8,236±7,294 U/ml, controls 137±128 U/ml;P<0.001). More advanced Rai's stages were related to higher soluble CD23 levels (P<0.01), both in typical and atypical B chronic lymphocytic leukemias, the highest levels and the best correlation with the absolute number of circulating CD19+ cells (r=0.50) being observed in the typical form. The number of membrane-bound CD23 molecules per cell was significantly higher in typical than in atypical B chronic lymphocytic leukemias (mean number 156,727±94,668 vs. 12,010±10,643,P<0.001). Our data suggest that soluble CD23 levels correlate with the clinical and biological features of leukemic B-cell lymphoproliferative disorders.

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References

  1. Thorley-Lawson D, Nadler LM, Bhar AK, Schooley RT. Blast-2 (EBV CS), an early cell surface marker of B cell activation, is super-induced by Epstein-Barr virus. J Immunol 1985; 123:3007.

    Google Scholar 

  2. Kintner C, Sugden B. Identification of antigenic determinants unique to the surfaces of cells transformed by Epstein-Barr virus. Nature 1981; 294:458.

    Article  PubMed  CAS  Google Scholar 

  3. Kikutani H, Suemura A, Owaki H, Nakamura H, Sato R, Yamasaki K, Barsumian E, Hardy R, Kishimoto T. Fce receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching. J Exp Med 1986 164:1455.

    Article  PubMed  CAS  Google Scholar 

  4. Gonzalez-Molina A, Spiegelberg HL. A subpopulation of normal human peripheral B lymphocytes that bind IgE. J Clin Invest 1977; 59:616.

    PubMed  CAS  Google Scholar 

  5. Nadler LM. B cell/leukemia panel workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte typing II, vol 2. New York Berlin Heidelberg: Springer; 1986:3.

    Google Scholar 

  6. Yukawa K, Kikutani H, Owaki H, Yamasaki K, Yokota A, Nakamura H, Barsumian EL, Hardy RR, Suemura M, Kishimoto T. A B cell-specific differentiation antigen, CD23, is a receptor for IgE (FceR) on lymphocytes. J Immunol 1987; 138:2576.

    PubMed  CAS  Google Scholar 

  7. Gordon J, Flores-Romo L, Cairns JA, Millsum MJ, Lane PJ, Johnson GD, MacLennan ICM. CD23: a multi-functional receptor/lymphokine? Immunol Today 1989; 10:153.

    Article  PubMed  CAS  Google Scholar 

  8. Delespesse G, Sarfati M, Hofstetter H. Human IgE-binding factor. Immunol Today 1989; 10:159.

    Article  PubMed  CAS  Google Scholar 

  9. Fournier S, Delespesse G, Rubio M, Biron G, Sarfati M. CD23 antigen regulation and signalling in chronic lymphocytic leukemia. J Clin Invest 1992; 89:1312.

    Article  PubMed  CAS  Google Scholar 

  10. Yokota A, Kikutani H, Tanaka T, Sato R, Barsumian E, Suemura M, Kishimoto T. Two species of human Fce receptor II (Fce-RII/CD23): tissue-specific and IL-4-specific regulation of gene expression. Cell 1988; 55:611.

    Article  PubMed  CAS  Google Scholar 

  11. Fournier S, Rubio M, Delespresse G, Sarfati M. Role for low-affinity receptor for IgE (CD23) in normal and leukemic B-cell proliferation. Blood 1994; 84:1881.

    PubMed  CAS  Google Scholar 

  12. Aubry JP, Pochon S, Graber P, Jansen KU, Bonnefoy JY. CD21 is a ligand for CD23 and regulates IgE production. Nature 1992; 358:505.

    Article  PubMed  CAS  Google Scholar 

  13. Saeland S, Duvert V, Moreau I, Banchereau J. Human B cell precursors proliferate and express CD23 after CD40 ligation. J Exp Med 1993; 178:113.

    Article  PubMed  CAS  Google Scholar 

  14. Galibert L, Durand I, Banchereau J, Rousset F. CD40-acivated surface IgD-positive lymphocytes constitute the long term IL-4-dependent proliferating B cell pool. J Immunol 1994; 152:22.

    PubMed  CAS  Google Scholar 

  15. Luo H, Hofstetter H, Banchereau J, Delespesse G. Cross-linking of CD23 antigen by its natural ligand (IgE) or by anti-CD23 antibody prevents B lymphocyte proliferation and differentiation. J Immunol 1991; 146:2122.

    PubMed  CAS  Google Scholar 

  16. Liu YJ, Cairns JA, Holder MJ, Abbot SD, Jansen KU, Bonnefoy JY, Gordon J, MacLennan ICM. Recombinant 25-kDa CD23 and interleukin la promote the survival of germinal cancer B cells: evidence for bifurcation in the development of centrocytes rescued from apoptosis. Eur J Immunol 1991; 21:1107.

    Article  PubMed  CAS  Google Scholar 

  17. Letellier M, Sarfati M, Delespesse G. Mechanism of formation of IgE-binding factors (soluble CD23). I. FceRII bearing B cells generate IgE-binding factors of different molecular weights. Mol Immunol 1990; 26:1105.

    Article  Google Scholar 

  18. Mossalayi MD, Lecron JC, Dalloul AH, Sarfti M, Bertho JM, Hofstetter H, Delespesse G, Debré P. Soluble CD23 (FceRII) and interleukin 1 synergistically induce early human thymocyte maturation. J Exp Med 1990; 171:959.

    Article  PubMed  CAS  Google Scholar 

  19. Mossalayi MD, Arock M, Bertho JM, Blanc C, Dalloul AH, Hofstetter H, Sarfati M, Delespesse G, Debré P. Proliferation of early human myeloid precursor induced by interleukin-1 and recombinant soluble CD23. Blood 1990; 75:1924.

    PubMed  CAS  Google Scholar 

  20. Yanagihara Y, Sarfati M, Marsh D, Nutman T, Delespesse G. Serum levels of IgE-binding factor (soluble CD23) in diseases associated with elevated IgE. Clin Exp Allergy 1990; 20:395.

    Article  PubMed  CAS  Google Scholar 

  21. Sarfati M, Bron D, Lagneaux L, Fonteyn C, Frost H, Delespesse G. Elevation of IgE-binding factors in serum of patients with B cell-derived chronic lymphocytic leukemia. Blood 1988; 71:94.

    PubMed  CAS  Google Scholar 

  22. Lowe J, Brown B, Hardie D, Richardson P, Ling N. Soluble forms of CD21 and CD23 antigens in the serum in B cell chronic lymphocytic leukemia. Immunol Lett 1989; 20:103.

    Article  PubMed  CAS  Google Scholar 

  23. Beguin Y, Lampertz S, De Groote D, Igot D, Malaise M, Fillet G. Soluble CD23 and other receptors (CD4, CD8, CD25, CD71) in serum of patients with chronic lymphocytic leukemia. Leukemia 1993; 7:2019.

    PubMed  CAS  Google Scholar 

  24. Rousselet G, Bachouchi M, Busson P, Vathaire F de, Wakasugi H, Schwaab G, Azli N, Armand J-P, Cvitkovic E, Tursz T. Clinical implications of the serum level of CD23 in patients with undifferentiated nasopharyngeal carcinoma. J Clin Oncol 1993; 11:2143.

    PubMed  CAS  Google Scholar 

  25. Bennett JM, Catovsky D, Daniel M-T, Flandrin G, Galton DAG, Gralnick HR, Sultan C, The French-American-British (FAB) Cooperative Group. Proposals for the classification of chronic (mature) B and T lymphoid leukemias. J Clin Pathol 1989; 42:567.

    PubMed  CAS  Google Scholar 

  26. Rai K, Sawitsky A, Cronkite EP, Chanana AD, Levy RN, Pasternack BS. Clinical staging of chronic lymphocytic leukemia. Blood 1975; 46:219.

    PubMed  CAS  Google Scholar 

  27. Rigby WFC, Waugh M, Graziano RF. Regulation of human monocyte HLA-DR and CD4 antigen expression, and antigen presentation by 1,25-dihydroxyvitamin D3. Blood 1990; 76:189.

    PubMed  CAS  Google Scholar 

  28. Huen DS, Henderson SA, Croom-Carter D, Rowe M. The Epstein-Barr virus latent membrane protein-1 (LMP1) mediates activation of NF-kB and cell surface phenotype via two effector regions in its carboxy-terminal cytoplasmic domain. Oncogene 1995; 10:549.

    PubMed  CAS  Google Scholar 

  29. Mosialos G, Birkenbach M, Yalamanchili R, VanArsdale T, Ware C, Kieff E. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 1995; 80:389.

    Article  PubMed  CAS  Google Scholar 

  30. Hu HM, O'Rourke K, Boguski MS, Dixit VM. A novel RING finger protein interacts with the cytoplasmic domain of CD40. J Biol Chem 1994; 269:30069.

    PubMed  CAS  Google Scholar 

  31. Thanos D, Maniatis T. NFkB: a lesson in family values. Cell 1995; 80:529.

    Article  PubMed  CAS  Google Scholar 

  32. Francia di Celle P, Carbone A, Marchis D, Zhou D, Sozzani S, Zupo S, Pini M, Mantovani A, Foa R. Cytokine gene expression in B-cell chronic lymphocytic leukemia: evidence of constitutive interleukin-8 (IL-8) mRNA expression and secretion of biologically active IL-8 protein. Blood 1994; 84:220.

    Google Scholar 

  33. Lantz O, Grillot-Courvalin C, Schmitt C, Fermand JP, Brouet JC. Interleukin-2 induced proliferation of leukemic human B cells. J Exp Med 1985; 161:1225.

    Article  PubMed  CAS  Google Scholar 

  34. Trentin L, Zambello R, Agostini C, Siviero F, Adami F, Marcolongo R, Raimondi R, Chisesi T, Pizzolo G, Semenzato G. Expression and functional role of TNF receptors on leukemic cells from patients with type B chronic lymphoproliferative disorders. Blood 1993; 81:752.

    PubMed  CAS  Google Scholar 

  35. Kolb JP, Renard D, Dugas B, Genot E, Petit-Koskas E, Sarfati M, Delespesse G, Poggioli J. Monoclonal anti-CD23 antibodies induce a rise in intracellular calcium and polyphosphoinositide hydrolysis in human activated B cells: involvement of a GP protein. J Immunol 1990; 145:429.

    PubMed  CAS  Google Scholar 

  36. Geisler CH, Larsen JK, Hansen NE, Hansen NM, Christensen BE, Lund B, Nielsen H, Plesner T, Thorling K, Andersen E, Andersen PK. Prognostic importance of flow cytometric immunophenotyping of 540 consecutive patients with B-cell chronic lymphocytic leukemia. Blood 1991; 78:1795.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Hematology, Verona University School of Medicine, Ospedale Policlinico, I-37134, Verona, Italy

    F. Vinante, A. Vassanelli, R. Zanotti, G. Nadali, M. Krampera, C. Vincenzi, L. Morosato & G. Pizzolo

  2. Department of Pathology, Verona University School of Medicine, Verona, Italy

    M. Chilosi

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  1. F. Vinante
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  2. A. Vassanelli
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  3. R. Zanotti
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  4. G. Nadali
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Vinante, F., Vassanelli, A., Zanotti, R. et al. Circulating levels of soluble CD23 reflect clinical and biological features of leukemic B-cell chronic lymphoproliferative disorders. Int J Clin Lab Res 25, 189–194 (1995). https://doi.org/10.1007/BF02592696

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  • DOI: https://doi.org/10.1007/BF02592696

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