Summary
Pyridoxal 5′-phosphate inhibited polymorphonuclear leukocyte aggregationin vitro in a dose-dependent fashion at concentrations ranging from 0.5 to 0.001 mmol/l. At pyridoxal 5′-phosphate concentrations between 0.4 and 0.5 mmol/l mean inhibition of aggregation was about 50%–60%. In this range, and at lower pyridoxal 5′-phosphate concentrations, phorbol myristate acetate-activated polymorphonuclear leukocytes showed a normal increase in volume, whereas swelling was inhibited at higher concentrations of pyridoxal 5′-phosphate. Since pyridoxal 5′-phosphate can be administered as a vitamin to man without any relevant side-effects its role as a physiological anti-aggregant of polymorphonuclear leukocytes warrants further investigation.
Similar content being viewed by others
References
Lisiewicz J. Human neutrophils. Maryland: Charles Press, 1980.
O'Flaherty JT, Kreutzer DL, Ward PA. Neutrophil aggregation and swelling induced by chemotactic agent. J Immunol 1977; 119: 232.
O'Flaherty JT, Dechatelet LR, McCall CE, Bass DA. Neutrophil aggregation: evidence for a different mechanism of action by phorbol myristate acetate. Proc Soc. Exp Biol Med 1980; 165: 225.
Craddock PR, Hammerschmidt D, White JG, Dalmasso AP, Jacob HS. Complement (C5a)-induced granulocyte aggregationin vitro. A possible mechanism of complement-mediated leukostasis and leukopenia. J Clin Invest 1977; 60:260.
Reed WP, Chick TW, Jutila K, Butler C, Goldblum S. Pulmonary leukostasis in fatal human pneumococcal bacteriemia without pneumonia. Am Rev Respir Dis 1984; 130: 1184.
Elliot CG, Zimmermann GA, Orme JF, Morris AH, Mortensen JD. Case report. Granulocyte aggregation in adult respiratory distress syndrome (ARDS)-serial histologic and physiologic observations. Am J Med Sic 1985; 289: 70.
Berliner S, Fishelson Z, Bruhis S, Kaufman H, Pinhas J. Aronson M. The phenomenon of leukergy: induction and detection of leukocyte aggregation in whole human blood. J Lab Clin Med 1987; 109:575.
O'Flaherty JT, Kreutzer DL, Showell HJ, Ward PA. Influence of inhibitors of cellular function on chemotactic factor-induced neutrophil aggregation. J Immunol 1977; 119: 1751.
Boucek MM, Snyderman R. Calcium influx requirement for human neutrophil chemotaxis: inhibition by lanthanum chloride. Science 1976; 193:905.
Oseas RS, Boxer LA, Butterick C, Baenher RL. Differences in polymorphonuclear leukocyte aggregating responses among several species in response to chemotactic stimulation. J Lab Clin Med 1980; 96:213.
O'Flaherty JT, Showell HJ, Kreutzer DL, Ward PA, Becker EL. Inhibition ofin vivo andin vitro neutrophil responses to chemotactic factors by a competitive agonist. J Immunol 1979; 120: 1326.
Ringertz B, Palmblad J, Lindgren JA. Stimulus-specific neutrophil aggregation: evaluation of possible mechanisms for the stimulus-response apparatus. J Lab Clin Med 1985; 106:132.
Shumer W. Steroids in the treatment of clinical septic shock. Ann Surg 1976; 184: 333.
Wilson JW. Treatment or prevention of pulmonary cellular damage with pharmacological doses of corticosteroids. Surg Gynecol Obstet 1972; 134: 675.
Hammerschmidt DE, White JG, Craddock PR. Corticosteroids inhibit complement-induced granulocyte aggregation. A possible mechanism for their efficacy in shocks states. J Clin Invest 1979; 63: 798.
O'Flaherty JT, Craddock PR, Jacobs HS. Mechanism of anticomplementary activity of corticosteroids in vivo: possible relevance in endotoxin shock. Proc Soc Exp. Biol Med 1977; 154:206.
Hammerschmidt DE, White JG, Jacob HS. Inhibition of granulocyte (PMN) aggregation: a possible mechanism of efficacy of corticosteroids in shock lung (ARDS) (abstracts). Clin Res 1978; 26:667A.
Nilsson E, Palmblad J. Effects of ethanol on mechanisms for secretory and aggregatory responses of human granulocytes. Biochem Pharmacol 1988; 37: 3237.
Cronstein BN, Stouwe M van de, Druska L, Levin R, Weissmann G. Non steroidal antiflammatory agents inhibit stimulated neutrophil adhesion to endothelium: adenosine dependent and independent mechanisms. Inflammation 1994; 18:323.
Kornecki E, Feinberg H. Possible mechanism of pyridoxal 5′-phosphate inhibition of ADP-induced aggregation. Fed Proc 1978; 37:546.
Subbarao K, Kuchibotla J, Kakkar VV. Pyridoxal 5′-phosphate—a new physiological inhibitor of blood coagulation and platelet function. Biochem Pharmacol 1979; 28: 531
Subbarao K, Kuchibotla J, Green D. Pyridoxine-induced inhibition of platelet aggregation and the release reaction. Circulation 1977; 56: III-77.
Bauernfeind JC, Miller ON. Vitamin B6: nutritional and pharmaceutical usage, stability, bioavailability, antagonists and safety. Human B6 requirements. Washington DC: National Academy of Sciences; 1978: 78.
Lippi U, Bellavite P, Schinella M, Nicoli M, Lippi G. Assessment of neutrophil aggregation by Coulter Counter STKR and STKS hematological analyzers. Clin Lab Haematol 1994; 16: 43.
Kornecki E, Feinberg H. Pyridoxal phosphate inhibition of platelet function. Am J Physiol 1980; 238: H54-H56.
Lam SCT, Harfenist EJ, Packham MA, Mustard JF. Investigation of platelet reactions. Thromb Res 1980; 20: 633.
Lippi U, Bellavite P, Schinella M, Nicoli M. Volumetric changes in phorbol myristate acetate activated neutrophils: a rapid and simple assay using Coulter Counter STKR and STKS hematological analyzers. Clin Lab Haematol 1994; 16: 43.
Schwartz BR, Harlan JM. Neutrophil membrane sulfhydryl groups are involved in stimulated neutrophil adherence to endothelium. J Leukoc Biol 1989; 45: 177.
Kraut EH, Sagone AL. A role for sulfhydryl groups in granulocyte aggregation. Blood 1989; 63: 1056.
Lippi U, Bellavite P, Schinella M, Nicoli M. Volume, conductivity and scatter changes of activated polymorphonuclear leukocytes: an estimation by Coulter Counter STKS analyzer. Int J Clin Lab Res 1992; 21: 321.
Lippi U, Schinella M, Nicoli M, Modena M, Lippi G. EDTA-induced platelet aggregation can be avoided by a new anticoagulant also suitable for automated complete blood count. Haematologica (Pavia) 1990; 75: 38.
Ratnaike S, Moss DW, Apoenzyme of aspartate aminotrans-ferases in serum in health and disease. Clin Chim Acta 1977; 74: 281.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schinella, M., Nicoli, M., Lippi, G. et al. Effect of pyridoxal 5′-phosphate on human neutrophil aggregationin vitro . Int J Clin Lab Res 25, 99–103 (1995). https://doi.org/10.1007/BF02592365
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02592365