Summary
An enzyme-linked immunoassay for human C4b-binding protein (concentration range 25–400 ng/ml) was developed using two monoclonal antibodies; the intra- and interassay coefficients of variation were less than 7.2%. In 50 normal subjects, 20 patients with liver cirrhosis and 34 full-term newborns, the plasma levels of C4b-binding protein were very similar to those measured by Laurell electroimmunoassay; in 24 patients with elevated erythrocyte sedimentation rates, levels measured by enzyme-linked immunoassay were higher then those measured by the Laurell method (270±70% vs. 223±67%). In these patients crossed immunoelectrophoresis showed a pattern different from that of normal individuals, which may explain the lower values found with the Laurell method.
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Coppola, R., Tombesi, S., Cristilli, P. et al. Comparison of two immunoassays for the complement protein C4b-binding protein in health and disease. Int J Clin Lab Res 25, 88–92 (1995). https://doi.org/10.1007/BF02592363
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DOI: https://doi.org/10.1007/BF02592363