Summary
The functional properties of two CD4+CD8−CD16−, five CD4−CD8+CD16− and three CD4−CD8−CD16+ human T cell clones were compared. All CD4− T cell clones displayed strong cytolytic activity in the lectin-dependent lytic assay against the P815 murine mastocytoma cell line, but only the CD4−CD8−CD16+ T cell clones exhibited lytic activity against the natural killer-sensitive K562 cell line. Upon activation with anti-CD3 monoclonal antibody, all T cell clones were able to support IgM and IgA synthesis in autologous B cells. Both CD4+ and CD4− T cell clones required cell-to-cell interaction with the B cells in order to exert their helper activity for immunoglobulin production. However, unlike CD4+, CD4−CD8+CD16− and CD4−CD8−CD16+ T cell clones provided helper function for immunoglobulin synthesis only when low T/B cell ratios were used in culture. At higher T/B cell ratios, there was a decline in the B cell helper activity of CD4− T cell clones that was probably related to the expression of cytolytic capacity against the antigen-presenting B cell. These data support the notion that under certain experimental conditions even cytotoxic T lymphocytes and natural killer cells may provide B cell helper function.
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Simonelli, C., Rugiu, F.S., Manetti, R. et al. Both CD8+ and CD16+ human T cell clones can provide B cell help for immunoglobulin production. Int J Clin Lab Res 22, 36–39 (1992). https://doi.org/10.1007/BF02591391
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DOI: https://doi.org/10.1007/BF02591391