Riassunto
Sono stati studiati gli effetti dell’insulina sulla capazione del glucosio da parte del grasso epididimale di ratto e sulla contrazione del muscolo striato di rana. Si è appurato che tanto l’azione metabolica quanto l’effetto meccanico potrebbero esplicarsi sullo stesso recettore a livello della membrana. Si adombra la possibilità che l’ormone agisca sopra un’ATPasi Ca++-, Mg++- e K+-dipendente, data la forte somiglianza tra l’azione dell’insulina e quella della ouabaina. Si ritiene che l’effetto dell’insulina potrebbe essere causato dall’interazione tra l’ormone e il recettore della membrana e dal suo effetto sui cationi divalenti Ca++ ed Mg++ che potrebbe alterate la conformazione della membrana cellulare. Il cambiamento di posizione dei cationi produce modificazioni del campo fisico-chimico che potrebbero a loro volta contribuire all’azione sulla membrana e scatenare le reazioni biochimiche endocellulari.
Résumé
On a étudié les effets de l’insuline sur l’absorption du glucose à niveau de la graisse épididymaire du rat et sur la contraction du muscle strié de la grenouille. On a trouvé que l’action sur le métabolisme et l’effet mécanique possèdent un récepteur commun à niveau de la membrane cellulaire. On suggère la possibilité que cet effet soit du à une ATPase sensible aux cations Ca++, Mg++ et K+, puisqu’on a remarqué une grande ressemblance entre l’action de l’insuline et celle de l’ouabaine. L’action de l’insuline pourrait se réaliser au moyen de sa fixation sur le récepteur de la membrane et sur les cations divalents Ca++ et Mg++, en provoquant une altération structurale de la membrane. Le changement de position des cations produit des altérations physico-chimiques qui peuvent non seulement avoir une action sur la membrane mais aussi déchaîner les réactions chimiques à l’intérieur de la cellule.
Resumen
Se estudia el efecto de la insulina sobre la capación de glucosa por la grasa epididimaria de la rata y sobre la contracción muscular estriada de la rana. Se encuentra que tanto la acción metabólica como la mecánica, puedan tener un receptor común a nivel de la membrana celular. Se sugiere la posibilidad de que el efecto sea sobre una ATPasa Ca++-, Mg++- y K+-dependiente, ya que se encontró similitud con la acción de la ouabaína. Se sugiere que la acción de la insulina pueda ser a través de su fijación en el receptor de la membrana y sobre los cationes divalentes Ca++ y Mg++ lo cual produce una alteración en la conformación de la membrana. La traslocación de los cationes provoca alteraciones en el campo fisicoquímico que además de contribuir a la acción sobre la membrana desencadena las reacciones bioquímicas en el interior de la célula.
Zusammenfassung
Der Verfasser untersuchte die Witkung von Insulin auf die Glukoseaufnahme des epididymalen Fettes der Ratte und auf die Kontraktion des gestreiften Froschmuskels. Es wurde festgestellt, dass die metabolische und die mechanische Wirkung einen gemeinsamen Rezeptor an der Zellmembran haben könnten, und es wird die Möglichkeit in Betracht gezogen, dass die Wirkung über eine Ca++-, Mg++- und K+-abhängige ATPase zustandekommt, da sie der Ouabainwirkung ähnlich ist. Weiterhin wird angenommen, dass der Insulineffekt durch die Wechselwirkungen zwischen dem Rezeptor der Zellmembran und den divalenten Kationen Ca++ und Mg++ zustandekommt, welche die Konformation der Zellmembran ändern. Die veränderte Stellung der Kationen führt zu Veränderungen des physikalisch-chemischen Zustandes, welche ebenfalls die Wirkung auf die Zellmembran beeinflussen und zur Auslösung der intrazellulären biochemischen Reaktionen beitragen konnten.
Summary
The effects of insulin on glucose uptake by rat epididymal fat pad and on frog striated muscle contraction were studied. It was found that both the metabolic action and the mechanical effect could have a common receptor at the membrane level. The possibility that the effect could be on a Ca++-, Mg++- and K+-dependent ATPase is suggested because a strong similarity between the actions of insulin and ouabain was found. It is thought that the action of insulin could be caused through its interaction with the membrane receptor and on the divalent cations Ca++ and Mg++ which might alter the conformation of the cellular membrane. The change in position of the cations produces alterations of the physicochemical field which might also contribute to the action on the membrane and trigger the intracellular biochemical reactions.
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