Lipids

, Volume 31, Issue 12, pp 1283–1288 | Cite as

The fatty acid composition of human gliomas differs from that found in nonmalignant brain tissue

  • Douglas D. Martin
  • Michael E. C. Robbins
  • Arthur A. Spector
  • B.-Chen Wen
  • David H. Hussey
Article

Abstract

To compare the fatty acid composition of tumor tissue from glioma patients with that of normal brain tissue, tissue samples were obtained from 13 glioma patients and from 3 nonmalignant patients. Following lipid extraction, total fatty acid composition was measured using gas-liquid chromatography. Samples were further separated into phospholipids and neutral lipids. Representative samples were then separated into phospholipid classes by thin-layer chromatography and the fatty acid composition assayed. Levels of the polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA), were significantly reduced (P=0.029) in the glioma samples compared with normal brain samples; mean values were 4.8±2.9% and 9.2±1.0%, respectively. This reduction in glioma DHA content was also observed in terms of phospholipids (4.6±2.1% vs. 9.6±0.8%,P=0.002). The phosphatidylserine and phosphatidylethanolamine phospholipid classes were reduced in the glioma samples. Differences were also noted in the n-6 PUFA content between glioma and normal brain samples. The glioma content of the n-6 PUFA linoleic acid was significantly greater (P<0.05) than that observed in the control samples in terms of total lipids. Thus, the fatty acid composition of human gliomas differs from that found in nonmalignant brain tissue.

Abbreviations

ALA

α-linolenic acid

CNS

central nervous system

DHA

docosahexaenoic acid

GBM

glioblastoma multiforme

GLC

gas-liquid chromatography

LA

linoleic acid

PC

phosphatidylcholine

PE

phosphatidylethanolamine

PI

phosphatidylinositol

PS

phosphatidylserine

PUFA

polyunsaturated fatty acid

UI

unsaturation index

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Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Douglas D. Martin
    • 1
  • Michael E. C. Robbins
    • 1
  • Arthur A. Spector
    • 2
  • B.-Chen Wen
    • 1
  • David H. Hussey
    • 1
  1. 1.Division of Radiation Oncology, Department of RadiologyThe University of Iowa Hospitals and ClinicsIowa City
  2. 2.Division of Radiation Oncology, Department of BiochemistryThe University of Iowa Hospitals and ClinicsIowa City
  3. 3.Radiation OncologyNaval Medical CenterPortsmouth

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