Summary
The isolated perfused rat pancreas was used to investigate the effect of oleic acid on glucagon secretion in response to 10 mmol/l arginine. In the absence of oleic acid and at 2.5 mmol/l calcium, arginine induced a biphasic glucagon secretion. At lower extracellular calcium concentration (1.0 mmol/l), the second phase of glucagon release was reduced, the first phase being unchanged. In the presence of 1,500 µmol/l oleic acid, the glucagon response to arginine was also biphasic, but second phase release was markedly inhibited, the first phase glucagon release being unchanged. Such an effect was not obtained when oleic acid concentration in the medium was 750 µmol/l. These results demonstrate that high concentrations of oleic acid inhibit glucagon secretion in response to arginine from the isolated perfused rat pancreas and support the concept that circulating free fatty acid levels are involved in the control of glucagon secretion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andrews S. S., Lopez S. A., Blackard W. G.: Effect of lipids on glucagon secretion in man — Metabolism24, 35, 1975.
Assan R., Attali J. R., Ballerio G., Boillot J., Girard J. R.: Glucagon secretion induced by natural and artificial aminoacids in the perfused rat pancreas — Diabetes26, 300, 1977.
Campillo J. E., Luyckx A. S., Torres M. D., Lefebvre P. J.: Effect of various concentrations of calcium on arginine-induced glucagon and insulin releasein vitro — Rev. esp. Fisiol.34, 191, 1978.
Campillo J. E., Luyckx A. S., Torres M. D., Lefebvre P. J.: Effect of oleic acid on insulin secretion by the isolated perfused rat pancreas — Diabetologia16, 267, 1979.
Dole V. P., Meinertz H.: Microdetermination of long chain fatty acids in plasma — J. biol. Chem.235, 2395, 1960.
Edwards J. C., Hellerström C., Petersson B., Taylor K. W.: Oxidation of glucose and fatty acids in normal and in A2-cell rich pancreatic islets isolated from guinea-pig — Diabetologia8, 93, 1972.
Edwards J. C., Howell S. L., Taylor K. W.: Fatty acids as regulators of glucagon secretion — Nature (London)224, 808, 1969.
Edwards J. C., Taylor K. W.: Fatty acids and the release of glucagon from isolated guineapig islets of Langerhans incubatedin vitro — Biochim. biophys. Acta (Amst.)215, 310, 1970.
Gerich J. E., Langlois M., Schneider V., Karam J. H., Noacca C.: Effect of alterations of plasma free fatty acid levels on pancreatic glucagon secretion in man — J. clin. Invest.53, 1284, 1974.
Gross R., Mialhe P.: Free fatty acid-glucagon feedback mechanism — Diabetologia10, 277, 1974.
Hicks B. H., Taylor C. I., Vij S. K., Pek S., Knopf R. F., Floyd J. C., Fajans S. S.: Effect of changes in plasma levels of free fatty acids on plasma glucagon, insulin and growth hormone in man — Metabolism26, 1011, 1977.
Laurent F., Mialhe P.: Effect of free fatty acids and aminoacids on glucagon and insulin secretions in normal and in diabetic ducks — Diabetologia15, 313, 1978.
Leclercq-Meyer V., Marchand J., Malaisse W. J.: The role of calcium in glucagon release. Interaction between arginine and calcium — Hormone Res.7, 348, 1976.
Luyckx A. S.: Immunoassay for glucagon — In:Lefebvre P. J., Unger R. H. (Eds): Glucagon. Molecular physiology, clinical and therapeutic implications. Pergamon Press, Oxford, 1972; p. 285.
Luyckx A. S.: Sécrétion de l’insuline et du glucagon. Étude clinique et expérimentale — Masson et Cie. Éd., Paris, 1974; p. 122.
Luyckx A. S., Gaspard U., Lefebvre P. J.: Influence of elevated plasma free fatty acids on the glucagon response to hypoglycemia in normal and in pregnant women — Metabolism27, 1033, 1978.
Luyckx A. S., Gerard J., Gaspard U., Lefebvre P. J.: Plasma glucagon levels in normal women during pregnancy. Diabetologia11, 549, 1975.
Luyckx A. S., Lefebvre P. J.: Arguments for a regulation of pancreatic glucagon secretion by circulating plasma free fatty acids. Proc. Soc. exp. Biol. (N.Y.)133, 524, 1970.
Luyckx A. S., Lefebvre P. J.: Effect of somatostatin on metabolic and hormonal changes induced by nicotinic acid in insulin-dependent diabetics — Diabetologia12, 447, 1976.
Madison L. L., Seyffert W. A., Unger R. H., Barker B.: Effect of plasma free fatty acids on plasma glucagon and serum insulin concentration — Metabolism17, 301, 1968.
Muller W. A., Aoki T. T., Flatt J. P., Blackburn G. L., Egdahl R. H., Cahill G. F. Jr.: Effect of β-hydroxybutyrate, glycerol and free fatty acid infusions on glucagon and epinephrine secretion in dogs during acute hypoglycemia — Metabolism25, 1077, 1976.
Quabbe H. J., Ramek W., Luyckx A. S.: Growth hormone, glucagon and insulin response to depression of plasma free fatty acids and the effect of glucose infusion — J. clin. Endocr.44, 383, 1977.
Seyffert W. A., Madison L. L.: Physiologic effect of metabolic fuels on carbohydrate metabolism. I. Acute effect of elevation of plasma free fatty acids on hepatic glucose output, peripheral glucose utilization, serum insulin and plasma glucagon levels — Diabetes16, 765, 1967.
Spector A. A.: Fatty acid binding to plasma albumin — J. Lipid Res.16, 165, 1975.
Tasaka Y., Sekine M., Yoshida Y., Hirata Y.: Effects of nicotinic acid on the plasma pancreatic glucagon, insulin and free fatty acids in diabetic subjects — Hormone metab. Res.8, 489, 1976.
Tiengo A., Muggeo M., Assan R.: Glucagon secretion in primary endogenous hypertriglyceridemia before and after clofibrate treatment — Metabolism24, 901, 1975.
Unger R. H., Lefebvre P. J.: Glucagon physiology — In:Lefebvre P. J., Unger R. H. (Eds): Glucagon. Molecular physiology, clinical and therapeutic implications. Pergamon Press, Oxford, 1972; p. 213.
Author information
Authors and Affiliations
Additional information
Presented in part at the 13th Annual Meeting of the European Association for the Study of Diabetes, Geneva, 28–30 September, 1977, and published in abstract form in Diabetologia13, 386, 1977.
Rights and permissions
About this article
Cite this article
Campillo, JE., Luyckx, A.S. & Lefebvre, P.J. Effect of oleic acid on arginine-induced glucagon secretion by the isolated perfused rat pancreas. Acta diabet. lat 16, 287–293 (1979). https://doi.org/10.1007/BF02587649
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02587649