Summary
In order to investigate the effects of two biguanide drugs, phenformin and metformin, on metabolic and hormonal changes with exercise, 6 healthy volunteers have been studied during and after 30 min of controlled moderate exercise on a bicycle ergometer on three occasions, two being for the drugs taken separately and the other without any drug, as a control study. Blood samples were taken for estimation of glucose, pyruvate, lactate, free fatty acids (FFA), glycerol, growth hormone (HGH) and insulin (IRI). Both drugs caused a greater rise in blood pyruvate and lactate concentrations during exercise compared with the changes in the control investigation. They also caused a delay in the disposal of pyruvate after exercise. In all investigations, the concentrations of FFA and glycerol rose to similar values at the end of exercise. In the control study, however, the pre-exercise value was achieved by the end of the investigation, whereas with the drugs the concentrations remained elevated. After exercise the rise of ketone-body concentrations was greater with the drugs. These observations indicate that the drugs interfere with aerobic utilization of metabolic fuels, reducing the utilization of pyruvate and glycerol and decreasing the rate of fat re-esterification: these metabolic effects are potentiated by exercise.
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Bergmeyer H. U., Bernt E.: D-Glucose: Determination with Glucose Oxidase and Peroxidase — In:Bergmeyer H. U. (Ed.): Methods of Enzymatic Analysis — Verlag Chemie, Weinheim, 1965; p. 123.
Connon J. J.: A Differential Action of Phenformin in Normal and Diabetic Rat Livers — Diabetologia9, 47, 1973.
Fajans S. S., Moorhouse J. A., Doorenboz H., Louis L. H., Conn J. W.: Metabolic Effects of Phenethylbiguanide in Normal Subjects and in Diabetic Patients — Diabetes9, 194, 1960.
Güttler F., Petersen F. B., Kjeldsen K.: The Influence of Phenformin on Blood Lactic Acid in Normal and Diabetic Subjects during Exercise — Diabetes12, 420, 1963.
Hohorst H., Kreutz F. H., Bücher T.: Über Metabolitgehalte und Metabolitkonzentrationen in der Leber der Ratte — Biochem. Z.332, 18, 1959.
Hultman E., Bergström J.: Muscle Glycogen Synthesis in Relation to Diet Studied in Normal Subjects — Acta med scand.182, 109, 1967.
Hunter W. M.: Control of Specificity in the Radioimmunoassay — In:Margoulies M. (Ed.): Protein and Polypeptide Hormones. Excerpta Medica Foundation, Amsterdam, 1969, p. 551.
Hunter W. M., Ganguli P. C.: The Separation of Antibody Bound from Free Antigen — In:Kirkham K. E., Hunter W. M. (Eds): Radioimmunoassay Methods — Churchill-Livingstone, Edinburgh, 1971; p. 243.
Itaya K., Ui M.: Colorimetric Determination of Free Fatty Acids in Biological Fluids — J. Lipid Res.6, 16, 1965.
Johnson R. H., Park D. M., Rennie M. J., Sulaiman W. R.: Hormonal Responses to Exercise in Racing Cyclists — J. Physiol. (Lond.)241, 23P, 1974.
Johnson R. H., Walton J. L., Krebs H. A., Williamson D. H.: Metabolic Fuels during and after Severe Exercise in Athletes and Non-Athletes — Lancet2, 452, 1969.
Kissebah A. H., Adams P. W., Wynn V.: Inter-Relationship between Insulin Secretion and Plasma Free Fatty Acid and Triglyceride Transport Kinetics in Maturity Onset Diabetes and the Effect of Phenethylbiguanide (Phenformin) — Diabetologia10, 119, 1974.
Kreutz F. H.: Enzymatische Glycerinbestimmung — Klin. Wschr.40, 362, 1962.
Mann H. B., Whitney D. R.: Test of Whether One of Two Random Variables Is Stochastically Larger Than the Other — Ann. Math. Statist.18, 52, 1947.
Morgan C. E., Lazarow A.: Immunoassay of Insulin: Two Antibody System — Diabetes12, 115, 1963.
Pereira J. N., Jangaard N. P., Pinson E. R.: Some Metabolic Effects of Phenformin in Rat Adipose Tissue — Diabetes16, 869, 1967.
Searle G. L., Cavalieri R. R.: Glucose Kinetics before and after Phenformin in the Human Subject — Ann. N.Y. Acad. Sci.148, 734, 1968.
Steiner D. F., Williams R. H.: Respiratory Inhibition and Hypoglycemia by Biguanides and Decamethylene-Biguanide — Biochim. biophys. Acta (Amst.)30, 329, 1958.
Williamson D. H., Mellanby J., Krebs H. A.: Enzymic Determination of D(-)-Hydroxybutyric and Acetoacetic Acid in Blood — Biochem. J.82, 90, 1962.
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Sulaiman, W.R., Johnson, R.H. The effects of phenformin and metformin on fat and carbohydrate metabolism. Acta diabet. lat 14, 129–136 (1977). https://doi.org/10.1007/BF02581400
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DOI: https://doi.org/10.1007/BF02581400