Summary
Fasting plasma cyclic adenosine monophosphate (cAMP) was measured in 50 matureonset diabetic patients and in 111 non-diabetic patients. Methods used to determine plasma cAMP are described. The addition of sepharose agar beads to the bovine adrenocortical binding protein has considerably improved the sensitivity and simplified the radioligand-receptor assay of cAMP. No statistical differences in plasma cAMP were noted in relation to sex in either group, to the presence of diabetes mellitus, or to age or weight in the non-diabetic patients. Plasma cGMP levels are now being studied to determine if these may prove better indicators of insulin activity than plasma cAMP.
Similar content being viewed by others
References
Cuatrecasas P.: Protein Purification by Affinity Chromatography — J. biol. Chem.245, 3059, 1970.
Fajans S. S., Conn J. W.: The Early Recognition of Diabetes Mellitus — Ann. N.Y. Acad. Sci.82, 208, 1959.
Fisch H. W., Pliska V., Schwyzer R.: Assay of Cyclo-3′,5′-Monophosphate (cAMP) in Sub-Picomole Quantities Using a Covalent Protein-Sepharose Complex — Experientia (Basel)28, 630, 1972.
Goldberg N. D.: Cyclic Nucleotides and Cell Function — Hosp. Pract.9, 127, 1974.
Hoffman W. S.: A Rapid Photoelectric Method for the Determination of Glucose — J. biol. Chem.120, 51, 1967.
Kaminsky N. I., Broadus A. E., Hardman J. G., Jones D. J. Jr., Ball J. H., Sutherland E. W., Liddle G. W.: Effects of Parathyroid Hormone on Plasma and Urinary Adenosine 3′,5′-Monophosphate in Man — J. clin. Invest.49, 2387, 1970.
Latner A., Prudhoe K.: A Simplified Competitive Protein Binding Assay for Adenosine 3′-5′-Monophosphate in Plasma — Clin. chim. Acta48, 353, 1973.
Liljenquist J. E., Bomboy J. D., Sinclair-Smith B. C., Lewis S. B., Felts P. W., Lacy W. W., Crofford O. B., Liddle G. W.: Net Splanchnic Cyclic AMP (cAMP) Production in Normal and Diabetic Men: Effects of Glucagon — J. clin. Invest.51, 58A, 1972.
Murad F., Weitzman R.: Hormonal Regulation of Cyclic AMP — Semin. Drug Treatm.3, 189, 1973.
Park C. R., Lewis S. B., Exton J. H.: Relationship of Some Hepatic Actions of Insulin to the Intracellular Level of Cyclic Adenylate — Diabetes21 (Suppl. 2), 439, 1972.
Rabinowitz B., Katz J.: Method for Determination of Cyclic AMP in Plasma — Clin. Chem.19, 312, 1973.
Soeldner J. A., Slone D.: Critical Variables in the Radioimmunoassay of Serum Insulin Using the Double Antibody Technic — Diabetes14, 771, 1965.
Tsang C. W. P., Lehotay D. C., Murphy B. E. P.: Competitive Binding Assay for Adenosine 3′,5′-Monophosphate Employing a Bovine Adrenal Protein: Application to Urine, Plasma and Tissues — J. clin. Endocr.35, 809, 1972.
Tucci J. R., Lin T., Kopp C.: Urinary Cyclic 3′,5′-Adenosine Monophosphate Levels in Diabetes Mellitus before and after Treatment — J. clin. Endocr.37, 832, 1973.
Wehmann R. E., Blonde L., Steiner A. L.: Sources of Cyclic Nucleotides in Plasma — J. clin. Invest.53, 173, 1974.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Drexler, J., Liu, A.C. & Foti, A.G. Fasting plasma cyclic AMP levels in an adult diabetic and non-diabetic group. Acta diabet. lat 14, 112–118 (1977). https://doi.org/10.1007/BF02581398
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02581398