Summary
Insulin binding studies performed in erythrocytes (RBC) have been employed in clinical studies assessing the status of insulin receptors at target cell tissues. However, some authors challenged this assumption on the basis of some discrepancies described in comparative studies of other cell types, probably related to populations of different cell age affecting insulin binding to RBC. We evaluated insulin binding to RBC in normal males (n=10), non-obese diabetic males (n=13), normal females (n=15), obese (n=11) and acromegalic females (n=5), before and after correction of insulin binding data for creatine concentration in the RBC as a procedure of correction for age, since a negative correlation was described between creatine content and RBC age which also correlates inversely with % insulin binding. Insulin binding in all three groups of patients was not statistically different from corresponding values for normal males and females respectively before correction of data for creatine, but significantly reduced values were found after adjustment for creatine in accordance with published data concerning monocytes. In conclusion, the procedure of correcting insulin binding in erythrocytes by the creatine content in RBC is potentially useful for clinical investigations, since the influence of RBC age is excluded.
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References
Arner P., Einarsson K., Backman L., Nilsell C., Lerea K. M., Livingston J. N.: Studies of liver insulin receptors in non-obese and obese human subjects — J. clin. Invest.72, 1729–1736, 1983.
Bar R. S., Gorden P., Roth J., Kahn R. C., De Meyts P.: Fluctuations in affinity and concentration of insulin receptors on circulating monocytes of obese patients: effect of starvation, refeeding and dieting — J. clin. Invest.58, 1123–1135, 1976.
Bolinder J., Östman J., Werner S., Arner P.: Insulin action in adipose tissue in acromegaly — J. clin. Invest.77, 1201, 1986.
Camagna A., De Pirro R., Tardella L., Rossetti L., Lauro R., Caprari P., Samoggia P., Salvo G.: Red blood cell age, pyruvate kinase activity and insulin receptors: evidence that monocytes and RBC may behave differently — Diabetes32, 1017–1022, 1983.
Corash L. M., Pionelli S., Chen H. C., Seamas C., Gross E.: Separation of erythrocytes according to age on a simplified density gradient — J. Lab. clin. Med.84, 147–151, 1974.
Dacie J. V., Lewis S. M. — Hematología Pratica, Ediciones Toray S.A., Barcelona, 1970; p. 31.
DeFronzo R. A., Ferrannini E., Koivisto V.: New concepts in the pathogenesis and treatment of non-insulin-dependent diabetes mellitus — Amer. J. Med.74, 52–81, 1983.
De Pirro R., Fusco A., Lauro R., Testa I., Ferreti F., De Martinis C.: Insulin receptors on monocytes and erythrocytes from obese patients — J. clin. Endocrinol. Metabol.51, 1437–1439, 1980.
De Pirro R., Fusco A., Lauro R., Testa I., Ferreti F., De Martinis C.: Erythrocyte insulin receptor in non-insulin-dependent diabetes mellitus — Diabetes29, 96–99, 1980.
Dons R. F., Corash L. M., Gorden P.: The insulin receptor is an age-dependent integral component of the human erythrocyte membrane — J. biol. Chem.256, 2982–2987, 1981.
Dons R. F., Ryan J., Gorden P., Wajchlicht-Rodbard H.: Erythrocyte and monocyte insulin binding in man. A comparative analysis in normal and disease states — Diabetes39, 896–902, 1981.
Fehr J., Knob M.: Comparison of red cell creatine levels and reticulocyte counts in appraising the severity of hemolytic process — Blood53, 966–967, 1979.
Gambhir K. K., Archer J. A., Carter L.: Insulin radioreceptor assay for human erythrocytes — Clin. Chem.23, 1590–1595, 1977.
Griffiths W. J.: Estimation of creatine in red cells — J. clin. Pathol.21, 412–413, 1968.
Hoffman W.: A rapid photoelectric method for the determination of glucose in urine and blood — J. biol. Chem.120, 51–55, 1937.
Kappy M. S., Plotnick L.: Erythrocyte insulin binding in obese children and adolescents — J. clin. Endocrinol. Metabol.51, 1440–1446, 1980.
Kolterman O. G., Gray R. S., Griffin J., Burstein P., Insel J., Scarlett J. A., Olefsky J. M.: Receptor and postreceptor defects contribute to the insulin resistance in non-insulin-dependent diabetes mellitus — J. clin. Invest.68, 957–969, 1981.
Kosmakos F. C., Nagulesparan M., Bennett P. H.: Insulin binding to erythrocytes: a negative correlation with red cell age — J. clin. Endocrinol. Metabol.51, 46–50, 1980.
Modan M., Karasik A., Halkin H., Fuchs Z., Lusky A., Shitrit A., Modan B.: Effects of past and concurrent body mass index on prevalence of glucose intolerance and type 2 (non-insulin-dependent) diabetes and on insulin response — Diabetologia29, 82–89, 1986.
Muggeo M., Bar R. S., Roth J., Kahn R., Gorden P.: The insulin resistance of acromegaly: evidence for two alterations in the insulin receptor on circulating monocytes — J. clin. Endocrinol. Metabol.48, 17–25, 1979.
Muggeo M., Dons R. F., Ryan J. L., Moghetti P., Valerio A.: Red blood cell insulin receptor assay in insulin receptors. In:De Pirro R. D., Lauro R. (Eds): Handbook on receptor research. Field Educational Italia, Rome, 1985; pp. 146–160.
Olefsky J. M.: Decreased insulin binding to adipocytes and circulating monocytes from obese subjects — J. clin. Invest57, 1165–1172, 1976.
Olefsky J. M., Reaven G. M.: Insulin binding in diabetes: relationships with plasma insulin levels and insulin sensitivity — Diabetes26, 680–688, 1977.
Peterson C. M., Koenig R. V., Jones R. L., Melvin E. T., Lehrman M. L.: Reversible hematologic sequela of diabetes mellitus — Ann. intern. Med.86, 425–429, 1977.
Polychronakos C., Ruggere M. D., Benjamin A., Posner B. I., Guyda H. J.: The role of cell age in the difference in insulin binding between adult and cord erythrocytes — J. clin. Endocrinol. Metabol.55, 290–294, 1982.
Thomopoulus P., Testa V., Flamier A., Berthelier M.: Insulin receptors and protein synthesis in bone marrow cells and reticulocytes — Diabetes29, 820–824, 1980.
Trivelli L. A., Ranney H. M., Lai H. T.: Hemoglobin components in patients with diabetes mellitus — New Engl. J. Med.284, 353–357, 1971.
Wajchenberg B. L., Lerario A. C., El-Andere W., Ohnuma L. Y., Souza I. T. T.: Human erythrocyte insulin receptors in normal male and female subjects — Clin. Endocrinol.20, 23–30, 1984.
Wajchenberg B. L., Pinto H., Souza I. T. T., Lerario A. C., Pieroni R. R.: Preparation of125-I labeled insulin for radioimmunoassay: comparison of lactoperoxidase and chloramine T iodination — J. nucl. Med.19, 900–905, 1978.
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This study was supported by a grant from Novo Research Institute, Bagsvaerd, Denmark.
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Lerario, A.C., El-Andere, W., Wajchenberg, B.L. et al. Erythrocyte insulin receptor: Normalization of binding data for the average cell age by the red cell creatine determination in obese, diabetic and acromegalic patients. Acta diabet. lat 25, 315–323 (1988). https://doi.org/10.1007/BF02581130
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DOI: https://doi.org/10.1007/BF02581130