Summary
The influence of increasing glucose concentrations on some essential functions in polymorphonuclear neutrophils (PMN) was evaluatedin vitro in 20 healthy persons. PMN chemotaxis, adherence, phagocytosis and bactericidal capacity were estimated. Higher than physiologic glucose concentrations were found to diminish chemotaxis, phagocytosis and bactericidal capacity. The results obtained may indicate another possible mechanism that accounts for the increased susceptibility to infection observed in diabetic patients. However, PMN adherence rose parallel with increasing glucose concentrations reaching maximum values at 300 mg/dl (16.6 mmol/l). The changes of PMN adherence may suggest the participation of these cells in the development of microangiopathic injuries.
Similar content being viewed by others
References
Andreani D.: Immunology in diabetes: introduction and overview. In:Andreani D., DiMario U., Federlin K. F., Heding L. G. (Eds): Immunology in diabetes. Kimpton Medical Publications, London-Edinburgh, 1984; p. 1.
Arquilla E. R., Stenger D.: Effects of diabetes on immune function. In:Andreani D., DiMario U., Federlin K. F., Heding L. G. (Eds): Immunology in diabetes. Kimpton Medical Publications, London-Edinburgh, 1984; p. 265.
Bagdade J. D., Root R. K., Bulger R. J.: Impaired leukocyte function in patients with poorly controlled diabetes — Diabetes23, 9, 1974.
Bagdade J. D., Stewart M., Walters E.: Impaired granulocyte adherence. A reversible defect in host defense in patients with poorly controlled diabetes — Diabetes27, 677, 1978.
Buschardt K.: Glycemic control and suppressor cell activity in patients with insulin-dependent diabetes mellitus — Clin. exp. Immunol.81, 189, 1982.
Buschardt K., Madsbad S., Rygaard J.: Suppressor cell activity in patients with newly diagnosed type I diabetes: a prospective study — J. clin. Lab. Immunol.8, 19, 1982.
Craddock P. R., Fehr J., Dalmasso A. P., Brigham K. L., Jacob H. S.: Hemodialysis leukopenia. Pulmonary vascular leukostasis resulting from complement activation by dialyzer cellophane membrane — J. clin. Invest.59, 879, 1977.
Dziatkowiak H., Kowaiska M., Denys A.: Phagocytic and bactericidal activity of granulocytes in diabetic children — Diabetes31, 1041, 1982.
Esmann V.: The polymorphonuclear leukocyte in diabetes — Fresenius’ Z. Analyt. Chem.311, 344, 1982.
Fehr J., Jacob H. S.:In vitro granulocyte adherence andin vivo margination: two associated complement-dependent functions. Studies based on the acute neutropenia of filtration leukapheresis — J. exp. Med.146, 641, 1977.
Fehr J., Moser P., Leppert D., Groscurth P.: Antiadhesive properties of biological surfaces are protective against stimulated granulocytes — J. clin. Invest.76, 535, 1985.
Fikrig S. M., Reddy C. M., Orti E., Herod D., Suntharalingam K.: Diabetes and neutrophil chemotaxis — Diabetes26, 466, 1977.
Pantazis C. G., Kniker W. T.: Assessment of blood leukocyte microbial killing by using a new fluorochrome microassay — J. reticuloendothel. Soc.26, 155, 1979.
Rayfield E. J., Ault M. J., Keusch M. J., Brothers M. J., Nechemias C., Smith H.: Infection and diabetes. The case for glucose control — Amer. J. Med.72, 439, 1982.
Repo H.: Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. I. Effects of environmental factors on neutrophil migration under agarose — Scand. J. Immunol.6, 203, 1977.
Wierusz-Wysocka B., Wysocki H., Siekierka H., Wykrętowicz A., Szczepanik A.: The plasma activity inducing polymorphonuclear neutrophils (PMN) aggregation, augmenting PMN adherence and chemotactic activity in patients with insulin-dependent diabetes — Immunol. pol.12, 65, 1987.
Wierusz-Wysocka B., Wysocki H., Siekierka H., Wykrętowicz A., Szczepanik A., Klimas R.: Evidence of polymorphonuclear neutrophils (PMN) activation in patients with insulin-dependent diabetes mellitus — Leukoc. Biol.42, 519, 1987.
Wierusz-Wysocka B., Wysocki H., Wykrętowicz A., Szczepanik A., Siekierka H.: Phagocytosis, bactericidal capacity and superoxide anion (O −2 ) production by polymorphonuclear neutrophils from patients with diabetes mellitus — Folia haematol. (Leipzig)5, 658, 1985.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wierusz-Wysocka, B., Wysocki, H., Wykrętowicz, A. et al. The influence of increasing glucose concentrations on selected functions of polymorphonuclear neutrophils. Acta diabet. lat 25, 283–288 (1988). https://doi.org/10.1007/BF02581126
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02581126