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On the mechanism of the insulin-glucose interactions

Acta diabetologia latina volume 16, pages 169–174 (1979)Cite this article

Summary

Analysis of the course of D-glucose binding to insulin has shown that the mechanism of glucose-insulin interaction is a cooperative process. Binding of D-glucose molecules to insulin is facilitated by the dissociation of insulin aggregates caused by insulin-glucose interaction. Thus, insulin behaves as a system with strong positive cooperativity. The results have been treated in accordance with theories for interactions coupled to association equilibria. The data obtained support the idea that insulin monomers are the active species responsible for insulin action.

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References

  1. Anzenbacher P., Kalous V.: Binding of D-glucose to insulin — Biochim. biophys. Acta (Amst.)386, 603, 1975.

    CAS  Google Scholar 

  2. Anzenbacher P., Kalous V.: Komůrkový aparát pro rovnovážnou dialysu — Chem. Listy69, 880, 1975.

    CAS  Google Scholar 

  3. Blundell T. L., Dodson G. G., Dodson E. J., Hodgkin D. C., Vijayan M.: Insulin, its chemistry and biochemistry — Recent Progr. Hormone Res.27, 1, 1971.

    PubMed  CAS  Google Scholar 

  4. Cuatrecasas P.: Insulin receptors of liver and fat cell membranes — Fed. Proc.32, 1838, 1973.

    PubMed  CAS  Google Scholar 

  5. Czech M. P.: Molecular basis of insulin action — Ann. Rev. Biochem.46, 359, 1977.

    PubMed  Article  CAS  Google Scholar 

  6. Dahlquist F. W.: The quantitative interpretation of maximum in Scatchard plot — FEBS Letters49, 267, 1974.

    PubMed  Article  CAS  Google Scholar 

  7. Grant P. T.: The conversion of proinsulin into insulin in the β-cells of mammals and fishes — Biochem. J.125, 49P, 1971.

  8. Henis Y. I., Levitzki A.: An analysis on the slope of Scatchard plots — Europ. J. Biochem.71, 529, 1976.

    PubMed  Article  CAS  Google Scholar 

  9. Hollenberg M. D., Cuatrecasas P.: Insulin interaction with membrane receptors and relationship to cyclic purine nucleotides and cell growth — Fed. Proc.34, 1556, 1975.

    PubMed  CAS  Google Scholar 

  10. Jeffrey P. D.: Polymerization behavior of bovine zinc-insulin at neutral pH. Molecular weight of the subunit and the effect of glucose — Biochemistry (Wash.)13, 4441, 1974.

    CAS  Google Scholar 

  11. Kahn C. R., Neville D. M. Jr., Roth J.: Insulin-receptor interaction in the obese hyperglycemic mouse. A model of insulin resistance — J. biol. Chem.248, 244, 1973.

    PubMed  CAS  Google Scholar 

  12. Klotz I. M.: Protein interactions with small molecules — Acc. Chem. Res.7, 162, 1974.

    Article  CAS  Google Scholar 

  13. Levitzki A., Schlessinger J.: Cooperativity in associating proteins. Monomer-dimer equilibrium coupled to ligand binding — Biochemistry (Wash.)13, 5214, 1974.

    CAS  Google Scholar 

  14. Maturo J. M. III: Isolation and partial characterization of a tryptophan-insulin complex — Endocrinology90, 947, 1972.

    PubMed  CAS  Article  Google Scholar 

  15. Maturo J. M. III, Diloff S.: Interaction between amino acids and insulin — Hormone metab. Res.5, 54, 1973.

    CAS  Article  Google Scholar 

  16. Milthorpe B. K., Nichol L. W., Jeffrey P. D.: The polymerization pattern of zinc(2)-insulin at pH 7.0 — Biochim. biophys. Acta (Amst.)495, 195, 1977.

    CAS  Google Scholar 

  17. Nichol L. W., Jackson W. J. H., Winzor D. J.: A theoretical study of the binding of small molecules to a polymerizing protein system. A model for allosteric effects — Biochemistry (Wash.)6, 2449, 1967.

    CAS  Google Scholar 

  18. Pullen R. A., Lindsay D. C., Wood S. P., Tickle I. S., Blundell T. L., Wollmer A., Krail G., Brandenburg D., Zahn H., Gliemann J., Gammeltoft S.: Receptor-binding region of insulin — Nature (Lond.)259, 369, 1976.

    Article  CAS  Google Scholar 

  19. Scatchard G.: The attractions of proteins for small molecules and ions — Ann. N.Y. Acad. Sci.51, 660, 1949.

    Article  CAS  Google Scholar 

  20. Scholtan W.: Die Bindung der Sulfonamide an Eiweisskörper — Arzneimittel-Forsch.14, 1139, 1964.

    CAS  Google Scholar 

  21. Schwarz G.: Some general aspects regarding the interpretation of binding data by means of a Scatchard plot — Biophys. Struct. Mechanism2, 1, 1976.

    Article  CAS  Google Scholar 

  22. Steinhardt J., Reynolds J. A.: Multiple equilibria in proteins — Academic Press, New York, 1969; p. 10.

    Google Scholar 

  23. Yalow R. S., Berson S. A.: Big, big insulin — Metabolism22, 703, 1973.

    PubMed  Article  CAS  Google Scholar 

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Affiliations

  1. Katedra fysikální chemie a katedra biochemie Přírodovědecká fakulta, Universita Karlova, Praha

    Vítěz Kalous & Pavel Anzenbacher

Authors
  1. Vítěz Kalous
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  2. Pavel Anzenbacher
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Kalous, V., Anzenbacher, P. On the mechanism of the insulin-glucose interactions. Acta diabet. lat 16, 169–174 (1979). https://doi.org/10.1007/BF02581096

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  • DOI: https://doi.org/10.1007/BF02581096

Key-words

  • Cooperativity of insulin interactions
  • Insulin
  • Insulin aggregates
  • Insulin-glucose binding
  • Insulin interactions