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Amylin immunoreactivity in the rat trachea and characterization of the interaction of amylin and somatostatin on airway mucus secretion

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Research in Experimental Medicine

Abstract

Amylin is a peptide containing 37 amino acids that is mainly expressed in pancreatic B-cells and cosecreted with insulin. It is the major component of the islet amyloid typically found in non-insulin-dependent diabetes mellitus. The amylin mRNA is present in RNA isolated from lung, and amylin receptors have been detected in lung membranes. Recently, amylin was shown to be a potent stimulator of airway mucus secretion. In this study, we characterized the site of amylin expression in rat trachea using a highly specific antiserum and the functional interaction of amylin with somatostatin-14 in mucus secreting cells. Amylin-like immunoreactivity is present in epithelial cells of submucous gland acini. The expression pattern varies, since some acini showed strong staining while others were negative. In addition, some columnar cells of the tracheal lining epithelium are strongly stained. Amylin applied submucosally is a potent stimulator of airway mucus secretion. Somatostatin inhibits this effect. Amylin may influence airway mucus secretion by paracrine and endocrine mechanisms, and our data suggest that amylin and somatostatin belong to the increasing number of peptides that are known to influence airway function.

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References

  1. Westermark P, Wernstedt C, Wilander E, Sletten K (1986) A novel peptide in the calcitonin gene related family as an amyloid fibril protein in the endocrine pancreas. Biochem Biophys Res Commun 140:827–831

    Article  PubMed  CAS  Google Scholar 

  2. Westermark P, Wernstedt C, O'Brien TD, Hayden DW, Johnson KH (1987) Islet amyloid in type 2 diabetes mellitus and adult diabetic cats contains a novel putative polypeptide hormone. Am J Pathol 127:414–418

    PubMed  CAS  Google Scholar 

  3. Cooper GJS, Willis AC, Clark A, Turner RC, Sim RB, Reid KBM (1987) Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients. Proc Natl Acad Sci USA 84:8628–8632

    Article  PubMed  CAS  Google Scholar 

  4. Sanke T, Bell GI, Sample C, Rubenstein AH, Steiner DF (1989) An islet amyloid peptide is derived from an 89-amino acid precursor by proteolytic processing. J Biol Chem 262:17243–17246

    Google Scholar 

  5. Westermark P, Wilander E, Westermark GT, Johnson KH (1987) Islet amyloid polypeptide-like immunoreactivity in the islet B cells of type 2 (non-insulin-dependent) diabetic and nondiabetic individuals. Diabetologia 30:887–892

    PubMed  CAS  Google Scholar 

  6. Johnson KH, O'Brien TD, Hayden DW, Jordan K, Ghobrial HKG, Mahoney WC, Westermark P (1988) Immunolocalization of islet amyloid polypeptide (IAPP) in pancreatic β cells by means of peroxidase-antiperoxidase (PAP) and protein A-gold techniques. Am J Pathol 130:1–8

    PubMed  CAS  Google Scholar 

  7. Fehmann HC, Weber V, Göke R, Göke B, Arnold, R (1990) Cosecretion of amylin and insulin from isolated rat pancreas. FEBS Lett 262:279–281

    Article  PubMed  CAS  Google Scholar 

  8. Ogawa A, Harris V, McCorkie SK, Unger RH, Luskey KL (1990) Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment. J Clin Invest 85:973–976

    PubMed  CAS  Google Scholar 

  9. Mosselman S, Höppener JWM, Lips JM, Jansz HS (1989) The complete islet amyloid polypeptide precursor is encoded by two exons. FEBS Lett 247:227–232

    Article  Google Scholar 

  10. Ferrier GJM, Pierson AM, Jones PM, Bloom SR, Girgis SI, Legon S (1989) Expression of the rat amylin (IAPP, DAP) gene. J Mol Endocrinol 3:R1-R4

    Article  PubMed  CAS  Google Scholar 

  11. Bhogal R, Smith DM, Bloom SR (1992) Investigation and characterization of binding sites for islet amyloid polypeptide in rat membranes. Endocrinology 130:906–913

    Article  PubMed  CAS  Google Scholar 

  12. Wagner U, Fehmann HC, Bredenbröker D, Yu F, Barth PJ, Wichert P von, Göke B (1993) The stimulatory effect of amylin on mucus secretion in isolated rat trachea. Res Exp Med 193:347–352

    CAS  Google Scholar 

  13. Wagner U, Fehmann HC, Bredenbröker D, Yu F, Barth PJ, Wichert P von (1995) Galanin and somatostatin inhibition of neurokinin A and B induced airway mucus secretion in the rat. Life Sci 57:283–289

    Article  PubMed  CAS  Google Scholar 

  14. Wagner U, Fehmann HC, Bredenbröker D, Yu F, Barth PJ, Wichert P von (1995) Galanin and somatostatin inhibition of substance P induced airway mucus secretion in the rat. Neuropeptides 28:59–64

    Article  PubMed  CAS  Google Scholar 

  15. Ussing HH, Zerhan K (1951) Active transport of sodium as a source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand 23:110–117

    PubMed  CAS  Google Scholar 

  16. Umeda Y, Arisawa M (1989) Characterization of cacitonin gene-related peptide (CGRP) receptors in guinea pig lung. Jpn J Pharmacol 51:377–384

    PubMed  CAS  Google Scholar 

  17. Wagner U, Wichert P von (1991) Control of mucus secretion in airways. Respiration 58:1–8

    Article  PubMed  CAS  Google Scholar 

  18. Ramnarine SI, Rogers DF (1994) Non-adrenergic, non-cholinergic neural control of mucus secretion in the airways. Pulm Pharmacol 7:19–33

    Article  PubMed  CAS  Google Scholar 

  19. Richter G, Feddersen O, Wagner U, Barth P, Göke R, Göke B (1993) GLP-1 stimulates secretion of macromolecules from airways and relaxes pulmonary artery. Am J Physiol 265:L374-L381

    PubMed  CAS  Google Scholar 

  20. Silvestre RA, Peir OE, Egano P, Miralles P, Marco J (1990) Inhibitory effect of rat amylin on the insulin responses to glucose and arginine in the perfused rat pancreas. Regul Pept 31:23–31

    Article  PubMed  CAS  Google Scholar 

  21. Molina JM, Cooper GJS, Leighton B, Olefsky JM (1990) Induction of insulin resistance in vivo by amylin and calcitonon gene-related peptide. Diabetes 39:260–265

    Article  PubMed  CAS  Google Scholar 

  22. Fürnsinn C, Leuvenink H, Roden M, Nowotny P, Schneider B, Rohac M, Pieber T, Clodi M, Waldhäusl W (1994) Islet amyloid polypeptide inhibits insulin secretion in conscious rats. Am J Physiol 267:E300-E305

    PubMed  Google Scholar 

  23. Leighton B, Foot E (1990) The effects of amylin on carbohydrate metabolism in skeletal muscle in vitro and in vivo. Biochem J 269:19–26

    PubMed  CAS  Google Scholar 

  24. Gomez-Foix M, Gil J, Rodriguez E, Guinovart JJ (1991), Anti-insulin effects of amylin and calcitonin-gene-related-eptide on hepatic glycogen metabolism. Biochem J 276:607–609

    PubMed  CAS  Google Scholar 

  25. Hartter E, Svobada T, Ludvik B, Schuller M, Kuenburg E, Brunnbauer W, Woloszczukw W, Prager R (1991) Basal and stimulated plasma levels of pancreatic amylin indicate its cosecretion with insulin in humans. Diabetologia 34:52–54

    Article  PubMed  CAS  Google Scholar 

  26. Johnson KH, O'Brien TD, Jordan K, Westermark P (1989) Islet amyloid, islet amyloid polypeptide and diabetes mellitus. N Engl J Med 321:513–517

    Article  PubMed  CAS  Google Scholar 

  27. Datta HK, Zaidi M, Wimalawanska MA, Ghatei MA, Beacham JL, Bloom SR, MacIntyre I (1989) In vivo and in vitro effect of amylin and amylin-amide on calcium metabolism in the rat and in the rabbit. Biochem Biophys Res Commun 162:2876–881

    Article  Google Scholar 

  28. Brain SD, Wimalawansa S, MacIntyre I, Williams JT (1990) The demonstration of vasodilator activity of pancreatic amylin amide in the rabbit. Am J Pathol 136:487–490

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Internal Medicine, Philipps University of Marburg, D-35033, Marburg, Germany

    Ulrich Wagner, Dirk Bredenbröker, Hans-Christoph Fehmann & Peter von Wichert

  2. Department of Pathology, Philipps University of Marburg, D-35033, Marburg, Germany

    Peter Josef Barth

  3. Clinical Research Unit for Gastrointestinal Endocrinology, Department of Internal Medicine, Philipps University of Marburg, Baldingerstrasse, D-35033, Marburg, Germany

    Hans-Christoph Fehmann

Authors
  1. Ulrich Wagner
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  2. Dirk Bredenbröker
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  3. Peter Josef Barth
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  4. Hans-Christoph Fehmann
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  5. Peter von Wichert
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Wagner, U., Bredenbröker, D., Barth, P.J. et al. Amylin immunoreactivity in the rat trachea and characterization of the interaction of amylin and somatostatin on airway mucus secretion. Res. Exp. Med. 195, 289–296 (1995). https://doi.org/10.1007/BF02576799

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  • DOI: https://doi.org/10.1007/BF02576799

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