Abstract
The reticuloendothelial system (RES) plays an important role in the biological defense system. In the liver, Kupffer cells are the main constituent of the RES, and when their function is impaired postoperative complications may more often occur. By using99mTc-labeled human serum albumin millimicrospheres (99mTc-HSA-MM) combined with assessment of single photon emission computed tomography (SPECT), we have attempted to determine the function of Kupffer cells independently of the hepatic blood flow. First, Kupffer cell function in rats with chronic liver injury caused by CCl4 was studied. The hepatic uptake rate in chronic liver injury was decreased, and a reduced phagocytic activity of the Kupffer cells was noted. The parameter concerning Kupffer cell degradation, the excretion rate (k), was markedly decreased in the early period of chronic liver injury. Changes in Kupffer cell function after 30% and 70% hepatectomy were also studied. After 30% hepatectomy, the excretion rate was decreased on the first postoperative day (POD), and it was increased beyond that found after sham operation on the 3rd POD. In contrast, slower recovery of uptake rate was demonstrated. After 70% hepatectomy, both uptake and excretion rates were markedly reduced, and recovery was prolonged beyond the 5th POD. The hepatic uptake was not parallel with the excretion rate in either experiment. These results suggest that the method that measures the hepatic excretion rate may provide a better assessment of Kupffer cell function than the current uptake measurement with radiolabeled colloid.
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References
Arii S, Shibagaki M, Takahashi S et al (1985) Changes in the reticuloendothelial phagocytic function after partial hepatectomy. J Lab Clin Med 105: 668–672
Aschoff L (1924) Das reticuloendotheliale System. Ergeb Inn Med Kinderheilkd 26: 1–118
Bolognesi M, Merkel C, Gatta A (1987) The use of human albumin millimicrospheres tagged with99mTc in the evaluation of the removal capacity of the reticuloendothelial system. Eur J Nucl Med 13: 254–257
Bradfield JWB (1980) Can we measure Kupffer cell function in man? In: Liehr H, Grun M (eds) The reticuloendothelial system and the pathogenesis of liver disease. Elsevier/North-Holland, Amsterdam, pp 309–316
De Nardo SJ, Bell GB, De Nardo GL (1976) Diagnosis of cirrhosis and hepatitis by quantitative hepatic and other reticuloendothelial clearance rates. J Nucl Med 17: 449–459
Goris RJA, Boekhorst TCA, Nuytineck JKS, Gimbrere JSF (1985) Multiple organ failure. Generalized autodestructive inflammation. Arch Surg 120: 1109–1115
Gross BK, Katz S, Dun SP, Cikrit D, Rosenthal R, Grosfeld, JL (1985) Bacterial clearance in the intact and regenerating liver. J Pediatr Surg 20: 320–323
Henson PM, Larsen GL, Webster RO, Mitchell BC, Goins AJ, Henson JE (1982) Pulmonary microvascular alterations and injury induced by complement fragments: synergistic effect of complement activation, neutrophil sequestration, and prostaglandins. Ann NY Acad Sci 384: 287–300
Higgins GM, Anderson RM (1981) Experimental pathology of the liver I. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12: 186–202
Keyes JW Jr, Wilson GA, Quinonest JD (1973) An evaluation of lung uptake of colloid during liver imaging. J Nucl Med 14: 687–691
Kim DK, Pfeifer J (1977) Measurement of phagocytic activity of reticuloendothelial system by intralipid. Surg Forum 28: 85–87
Kuratsune H, Koda T, Kurahori T (1983) The relationship between endotoxin and the phagocytic activity of the reticuloendothelial system. Hepatogastroenterology 30: 78–82
Mimura H, Takakura N, Kim H, Ohno Y, Hamazaki K, Matsuda T, Tsumura M, Toda S, Hiraki Y (1986) Determination of the extent of feasible hepatic resection from hepatic blood flow. World J Surg 10: 302–310
Pearson HJ, Chamberlain J, Anderson J, Bowry V, Bell PRF (1985) Can technetium-labelled millimicrospheres be used to measure Kupffer-cell function? An experimental study. Eur J Nucl Med 10: 358–361
Peters T, Karck U, Decker K (1990) Interdependence of tumor necrosis factor, prostaglandin E2, and protein synthesis in lipopolysaccharide-exposed rat Kupffer cells. Eur J Biochem 191: 583–589
Reske SM, Vyska K, Feinendegen LE (1981) In vivo assessment of phagocytic properties of Kupffer cells. J Nucl Med 22: 405–410
Rimola A, Soto R, Bory F (1984) Reticuloendothelial system phagocytic activity in cirrhosis and its relation to bacterial infection and prognosis. Hepatology 4: 53–58
Roumen RM, Hendriks T, Ven-Jongekrijg J, Nieuwenhuijzen GAP, Sauerwein RW, Meer JWM, Goris RA (1993) Cytokine patterns in patients after major vascular surgery, hemorrhagic shock, and severe blunt trauma. Relation with subsequent adult respiratory distress syndrome and multiple organ failure. Ann Surg 218: 769–776
Saba TM (1970) Physiology and physiopathology of the reticuloendothelial system. Arch Intern Med 126: 1031–1352
Saba TM (1970) Liver blood flow and intravascular colloid clearance alteration following partial hepatectomy. J Reticuloendothel Soc 7: 406
Seyer JM (1980) Interstitial collagen polymorphism in rat liver with CCl4-induced cirrhosis. Biochim Biophys Acta 629: 490–498
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Hamazaki, K., Sato, S., Yunoki, M. et al. Kupffer cell function in chronic liver injury and after partial hepatectomy. Res. Exp. Med. 194, 237–246 (1994). https://doi.org/10.1007/BF02576385
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DOI: https://doi.org/10.1007/BF02576385