Skip to main content
SpringerLink
Log in

Effect of hypoxic and carbon monoxide-induced hypoxia on regional myocardial segment work and O2 consumption

  • Original Papers
  • Published:
Research in Experimental Medicine

Abstract

The purpose of this study was to investigate the potentially greater responses of regional myocardial work and O2 consumption to hypoxic hypoxia than to CO-induced hypoxia. Twenty open-chest anesthetized dogs were studied under control and four hypoxic conditions, hypoxic hypoxia induced with either 8% O2 (SaO2=56%) or 6% O2 (SaO2=40%) gas mixtures, or CO-induced hypoxia produced by a 1% CO gas mixture for either 7 min (SaO2=67%; SaCO=30%) or 20 min (SaO2=40%; SaCO=56%). Ultrasonic crystals and a force gauge were utilized to measure myocardial shortening and force. Regional myocardial segment work was calculated by integrating myocardial segment shortening multiplied by its corresponding force. Radioactive micropheres were used to measure regional coronary blood flow during each condition. Transmural biopsies were utilized to measure arterial and venous O2 saturation with a four-wavelength microspectrophotometric method. Regional O2 extraction and consumption were calculated Regional coronary blood flow (77±38 ml/min per 100 g, control) increased with severe hypoxic hypoxia (293±206) and CO-induced hypoxia (150±128). Regional myocardial O2 extraction was decreased with both hypoxic and CO hypoxia. Regional myocardial O2 consumption was maintained even with severe hypoxic and CO hypoxia. Regional myocardial segment work/min increased from 343±205 g*mm/min with increasing levels of hypoxic hypoxia (564±677) and decreased with increasing levels of CO hypoxia (169±111). Regional segment work increased with increasing levels of hypoxic hypoxia and decreased with increasing CO hypoxia. The differential effects on segment work caused by the two types of hypoxia may be due to direct metabolic or autonomic differences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adachi H, Strauss HW, Ochi H, Wagner HN Jr (1976) Effect of hypoxia on the regional distribution of cardiac output in the dog. Circ Res 39:314–319

    PubMed  CAS  Google Scholar 

  2. Adams JD, Erickson HH, Stone HL (1973) Myocardial metabolism during exposure to carbon monoxide in the conscious dog. J Appl Physiol 34: 238–242

    PubMed  CAS  Google Scholar 

  3. Buckberg GD, Luck JC, Payne DE, Hoffman JIE, Archie JP, Fixler DE (1971) Some sources of error in measuring regional blood flow with radioactive microspheres. J Appl Physiol 31: 598–604

    PubMed  CAS  Google Scholar 

  4. Cain SM (1977) Oxygen delivery and uptake in dogs during anemic and hypoxic hypoxia. J Appl Physiol 42: 228–234

    PubMed  CAS  Google Scholar 

  5. Daly MDeB, Scott MJ (1964) The cardiovascular effects of hypoxia in the dog with special reference to the contribution of the carotid body chemoreceptors. J Physiol (Lond) 273: 201–214

    Google Scholar 

  6. DeDeest H, Levy MN, Zieske H (1965) Reflex effects of cephalic hypoxia, hypercapnia, and ischemia upon ventricular contractility. Circ Res 17: 349–358

    Google Scholar 

  7. Downing SE, Mitchell JA, Wallace AG (1963) Cardiovascular responses to ischemia, hypoxia and hypercapnia of the central nervous system. Am J Physiol 204: 881–887

    Google Scholar 

  8. Einzig S, Nicoloff DM, Lucas RV Jr (1980) Myocardial perfusion abnormalities in carbon monoxide-poisoned dogs. Can J Physiol Pharmacol 58: 396–405

    PubMed  CAS  Google Scholar 

  9. Gewirtz H, Brautigan DL, Olsson RA, Brown P, Most AS (1983) Role of adenosine in the maintenance of coronary vasodilation distal to a severe coronary artery stenosis. Circ Res 53: 42–51

    PubMed  CAS  Google Scholar 

  10. Heller LJ, Trachte GJ, Regal JF (1989) Role of adenosine in hypoxic alterations of anaphylaxis of isolated guinea pig hearts. Am J Physiol 257: H1378-H1388

    PubMed  CAS  Google Scholar 

  11. Hogan MC, Bebout DE, Gray AT, Wagner PD, West JB, Haab PE (1990) Muscle maximal O2 uptake at constant O2 delivery with and without CO in the blood. J Appl Physiol 69: 830–836

    PubMed  CAS  Google Scholar 

  12. Hoka S, Bosnjak ZJ, Arimura H, Kampine JP (1989) Regional venous outflow, blood volume and sympathetic nerve activity during severe hypoxia. Am J Physiol 256: H162-H170

    PubMed  CAS  Google Scholar 

  13. Kaijser L, Grubbstrom J, Berglund B (1990) Coronary circulation in acute hypoxia. Clin Physiol 10: 259–263

    PubMed  CAS  Google Scholar 

  14. Kampen EJ, Zijlstra WG (1965) Determination of hemoglobin and its derivatives. Adv Clin Chem 8: 141–187

    PubMed  Google Scholar 

  15. Kedem J, Weiss HR, Scholz PM (1991) Augmented efficiency of regional myocardial work by ouabain. Cardiovasc Res 25: 916–922

    PubMed  CAS  Google Scholar 

  16. Kedem J, Sonn J, Scheinowitz M, Weiss HR (1992) Effect of isoproterenol on regional myocardial segment work, O2 consumption and oxygen balance. Res Exp Med 192: 323–334

    CAS  Google Scholar 

  17. King CE, Cain SM, Chapler CK (1983) Whole body and hindlimb cardiovascular responses of the anesthetized dog during CO hypoxia. Can J Physiol Pharmacol 62: 769–774

    Google Scholar 

  18. King CE, Dodd SL, Cain SM (1987) O2 delivery to contracting muscle during hypoxic or CO hypoxia. J Appl Physiol 63: 726–732

    PubMed  CAS  Google Scholar 

  19. Kontos HA, Levasseur JE, Richardson DW, Mauck HP Jr, Patterson JL Jr (1967) Comparative circulatory responses to systemic hypoxia in man and in unanesthetized dogs. J Appl Physiol 23: 381–386

    PubMed  CAS  Google Scholar 

  20. Krasney JA, Magno MG, Levitzky MG, Koehler RC, Davis DG (1971) Cardiovascular responses to arterial hypoxia in awake sinoaortic denervated dogs. Am J Physiol 220: 1361–1366

    PubMed  CAS  Google Scholar 

  21. Krasney JA, Koehler RC (1977) Influence of arterial hypoxia on cardiac dynamics in the conscious sinoaortic-denervated dog. J Appl Physiol 43: 1012–1018

    PubMed  CAS  Google Scholar 

  22. Lee SC, Mallet RT, Shizukuda Y, Williams AG Jr, Downey HF (1992) Canine coronary vasodepressor responses to hypoxia are attenuated but not abolished by 8-phenyltheophylline. Am J Physiol 262: H955-H960

    PubMed  CAS  Google Scholar 

  23. Mark AL, Abboud FM, Heistad DD, Schmid PG, Johannsen J (1974) Evidence against the presence of ventricular chemoreceptors activated by hypoxia and hypercapnia. Am J Physiol 227: 178–182

    PubMed  CAS  Google Scholar 

  24. Mazer CD, Stanley WC, Hickey RF, Neese RA, Caspm BA, Demas KA, Wisneski JA, Gertz EW (1990) Myocardial metabolism during hypoxia maintained lactate oxidation during increased glycolysis. Metab Clin Exp 39: 913–918

    PubMed  CAS  Google Scholar 

  25. Merrill GF, Downey HF, Yonekura S, Watanabe N, Jones CE (1988) Adenosine deaminase attenuates canine coronary vasodilation during regional non-ischaemic myocardial hypoxia. Cardiovasc Res 22: 345–350

    Article  PubMed  CAS  Google Scholar 

  26. Powers ER, Powell WJ Jr (1973) Effect of arterial hypoxia on myocardial oxygen consumption. Circ Res 33: 749–756

    PubMed  CAS  Google Scholar 

  27. Rothe CF, Maass-Moreno R, Flanagan AD (1990) Effects of hypercapnia and hypoxia on the cardiovascular system: vascular capacitance and aortic chemoreceptors. Am J Physiol 259: H932-H939

    PubMed  CAS  Google Scholar 

  28. Scharf SM, Permutt S, Bromberger-Barnea B (1975) Effects of hypoxic and CO hypoxia on isolated hearts. J Appl Physiol 39: 752–758

    PubMed  CAS  Google Scholar 

  29. Walley KR, Becker CJ, Hogan RA, Teplinsky K, Wood LDH (1988) Progressive hypoxemia limits left ventricular oxygen consumption and contractility. Circ Res 63: 849–859

    PubMed  CAS  Google Scholar 

  30. Wasicko MJ, Melton JE, Neubauer JA, Krawein N, Edelman NH (1990) Cervical sympathetic and phrenic nerve responses to progressive brain hypoxia. J Appl Physiol 68: 53–58

    PubMed  CAS  Google Scholar 

  31. Weiss HR, Neubauer JA, Lipp JA, Sinha AK (1978) Quantitative determination of regional oxygen consumption in the dog heart. Circ Res 42: 394–401

    PubMed  CAS  Google Scholar 

  32. Zhu NH, Weiss HR (1991) Oxy-and carboxyhemoglobin saturation determination in frozen small vessels. Am J Physiol 260: H626-H631

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Heart and Brain Circulation Laboratory, Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, NJ, USA

    Naihong Zhu & Harvey R. Weiss

Authors
  1. Naihong Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harvey R. Weiss
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, N., Weiss, H.R. Effect of hypoxic and carbon monoxide-induced hypoxia on regional myocardial segment work and O2 consumption. Res. Exp. Med. 194, 97–107 (1994). https://doi.org/10.1007/BF02576370

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02576370

Key words

Search

Navigation