Summary
Foreign body reaction of the liver, kidney, and subcutaneous tissue of rats after implantation of hollow fibers (HF) for 1, 3, 6, and 12 months and its influence on the effectiveness of diffusional transport of insulin and oxygen were investigated. The highest degree of fibrosis was observed after subcutaneous implantation of HF and the lowest degree after implantation into the kidney. Histochemical staining of the fibrous capsule showed a tissue-dependent domination of the collagenous fibrils. After 90 min of perfusion 33% of the insulin contained in HF diffused out of the nonimplanted fiber, after 120 min, 73% and after 180 min, 100%. Hollow fibers, which were removed with a surrounding connective capsule after an implantation period of 1 year, showed even better permeability for insulin than nonimplanted HF. The tension of oxygen in the lumen of the implanted hollow fibers was 42 mm Hg after implantation into the kidney and 30 mm Hg after implantation into the liver. The oxygen present inside an HF that is implanted into a kidney and liver was consumed by 100 islets in 3.9 and 2.8 min, respectively. It was concluded that to achieve acceptable results in the construction of bioartificial pancreas more research activities should be performed on the diffusion and consumption of oxygen in the bioreactor.
Similar content being viewed by others
References
Altman JJ, Houlbert D, Callard P, McMillan P, Solomaon BA, Rosen J, Galleti PM (1986) Long-term plasma glucose normalization in experimental diabetic rats with macroencapsulated implants of benign human insulinomas. Diabetes 35:625–633
Asplund K, Hellerström C (1972) Glucose metabolism of pancreatic islets isolated from neonatal rats. Horm Metab Res 4:159–163
Bodziony J, Schwille PO (1985) Islet isolation from atrophied pancreas after short-term duct ligature-evidence for normal in vitro release of insulin and somatostatin. Horm Metab Res 17:314–316
Bodziony J, Schwille PO (1988) Verwendung von Hohlfasern zur Entwicklung des bioartifizellen Pankreas. Akt Endokrin Stoffw 9:96
Bodziony J, Schwille PO (1989) In vitro secretion of hormones from the islets of pancreas with acinar atrophy-influence of digestion with collagenase. Exp Clin Endocrinol 94:338–344
Bodziony J, Kaminski K, Matuszewski W, Reiss J, Waronski W (1976) Anpassung der Sauerstoffelektrode des Astrupapparats AME-1 an die Messung der Aktivität sauerstoffverbrauchender Enzyme. Z Med Labortechnik 17:192–197
Bodziony J, Gasior U, Stanosek J (1985) Funktionsbeurteilung isolierter Langerhansscher Inseln in semipermeablen Kammern. Z Exp Chir Transplant Künstliche Organe 18:204–214
Burck HC (1973) Histologische Technik. Thieme, Stuttgart
Clark G (ed) (1981) Staining procedures. William & Wilkins, Baltimore
Gray DWR, Morris PJ (1987) Developments in isolated pancreatic islet transplantation. Transplanatation 43:321–331
Hedeskov CJ, Hertz L, Nissen C (1972) The effect of mannoheptulose on glucose and pyruvate-stimulated oxygen uptake in normal mouse pancreatic islets. Biochim Biophys Acta 261:388–397
Hutton JC, Malaisse WJ (1980) Dynamics of O2 consumption in rat pancreatic islets. Diabetologia 18:395–405
Jolley WB, Hinshaw DB, Call TW, Alvord LS (1977) Xenogeneic pancreatic islet transplantation in proteolytic enzyme-bonded diffusion chambers in diabetics rats. Transplant Proc 9:363–365
Panten U, Klein H (1982) Oxygen consumption by isolated pancreatic islets, as measured in a microincubation system with a Clark-type electrode. Endocrinology 111:1595–1600
Pearse A (1986) Histochemistry. Theoretical and applied (vol 1). J Churchil, London
Radiometer (1980) Instruction manual for pO2 electrode, type E5046. Radiometer, Copenhagen
Reach G (1984) Bioartificial pancreas. Present state and future prospects. Biomed Biochim Acta 43:569–576
Robb-Smith AHT (1957) In: Tunbridge ER, Keech M, Delafresnaye JF, Wood GC (eds) Connective tissue. Blackwell Scientific, Oxford
Scharp DW, Mason NS, Sparks RE (1984) Islet immuno-isolation: the use of hybrid artificial organs to prevent islet tissue rejection. World J Surg 8:221–229
Scharp WD, Lacy PE, Santiago JV, McCullogh CS, Weide LG, Falqui L, Marchetti P, Gingerich R, Jaffe AS, Cryer PE, Andersoin CB, Flye MW (1990) Insulin independence after islet transplantation into type I diabetic patient. Diabetes 39:515–518
Silver F, Doillon C (1989) Biocompatibility. VCH, New York Wieheim Cambridge
Sorensen J, Colton CK, Hillman RS, Soeldner JS (1982) Use of a physiologic pharmacokinetic model of glucose homeostatis for assessment of performance requirements for insulin therapy. Diabetes Care 5:148–157
Theodorou NA, Vrbova H, Tyhurst M, Howell SL (1980) Problems in the use of polycarbonate diffusion chambers for syngeneic pancreatic islet transplantation in rats. Diabetologia 18:313–317
Zekorn T, Komp U, Bretzel RG, Federlin K (1986) Islet transplantation in experimental diabetes of the rat. XI: In vitro tests on artificial membrane applied in islet transplantation. Horm Metab Res 19:87–88
Zekorn T, Siebers U, Filip L, Mauer K, Schmitt U, Bretzel RG, Federlin K (1989) The use of different hollow fibers as a diffusion chamber. Transplant Proc 21:2748–2750
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bodziony, J. Bioartificial endocrine pancreas: foreign-body reaction and effectiveness of diffusional transport of insulin and oxygen after long-term implantation of hollow fibers into rats. Res. Exp. Med. 192, 305–316 (1992). https://doi.org/10.1007/BF02576287
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02576287