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Changes in membrane-bound hydrolases by metronidazole in rat renal brush border

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Research in Experimental Medicine

Summary

The antiprotozoal drug metronidazole, when administered orally at a dose level of 100 mg/kg body wt. daily for 7 days to rats, brought about significant elevation of renal brush-border-membrane-bound hydrolytic enzymes, such as alkaline phosphatase, maltase, sucrase, and leucine aminopeptidase (LAP). Kinetic analysis of the enzymes (substrate saturation) indicated that the drug produced an increase in the maximum of apparent initial enzyme velocity (V max), while the substrate affinity constant (K m) remained unaltered. These changes were not recovered to the normal level event after the drug regimen was stopped and the animals were allowed to recover for a period of 7 days. Lipid analysis of brush border membrane (BBM) revealed a significant elevation in the cholesterol, phospholipid, and ganglioside levels, while no marked change was recorded in triglyceride, free fatty acid and plasmalogen. Study of the temperature-dependent parameters of the enzymes showed that metronidazole induced a shift in the transition temperature (T o) in LAP with nearly total reversibility in the recovery group. No such change was seen in the other enzymes. However, there also was a lowering in the energy of activation (E a) belowT o, which returned to normal after the treatment was withdrawn.

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Authors and Affiliations

  1. Department of Biophysics, Panjab University, 160014, Chandiagrh, India

    Leelank Jamba & Shankar N. Sanyal

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  1. Leelank Jamba
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  2. Shankar N. Sanyal
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Jamba, L., Sanyal, S.N. Changes in membrane-bound hydrolases by metronidazole in rat renal brush border. Res. Exp. Med. 192, 287–294 (1992). https://doi.org/10.1007/BF02576285

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  • DOI: https://doi.org/10.1007/BF02576285

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