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Regulation of NK cell activity and the level of the intracellular cAMP in human peripheral blood lymphocytes by Met-enkephalin

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Research in Experimental Medicine

Summary

The effect of Met-enkephalin on natural killer (NK) activity and on intracellular cyclic adenosine monophosphate (cAMP) level in human peripheral blood lymphocytes was measured 1/4, 1, 2, and 24h after incubation. Concentrations of Met-enkephalin ranged from 10−12 to 10−8 M. Cyclic changes in NK activity and in the intracellular cAMP level after treatment with Met-enkephalin were observed. Kinetics of changes caused by high (10−9, 10−8 M) and low concentrations (10−12, 10−11, 10−10 M) of Met-enkephalin differed from each other. Early, nearly threefold increase in the level of intracellular cAMP was found 15 min after treating peripheral blood lymphocytes with 10−12 M Met-enkephalin. By contrast, a nearly 75% decrease of intracellular cAMP level was found 2 h after treatment with 10−9 M Met-enkephalin. Generally, early intensive changes in the cAMP level in peripheral blood lymphocytes, induced by Met-enkephalin, were followed by delayed, subtle changes in NK cell activity in the opposite direction.

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Authors and Affiliations

  1. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia

    I. Martin-Kleiner, M. Osmak & J. Gabrilovac

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  1. I. Martin-Kleiner
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  2. M. Osmak
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  3. J. Gabrilovac
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Martin-Kleiner, I., Osmak, M. & Gabrilovac, J. Regulation of NK cell activity and the level of the intracellular cAMP in human peripheral blood lymphocytes by Met-enkephalin. Res. Exp. Med. 192, 145–150 (1992). https://doi.org/10.1007/BF02576269

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  • DOI: https://doi.org/10.1007/BF02576269

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