Summary
To ascertain whether the effects of diet on glucose tolerance and insulin sensitivity are mediated through changes of insulin receptors we have studied insulin binding to monocytes in 24 young volunteers (4 groups of 6) during 2 week periods of different dietary regimens. In group 1 and 2 the subjects had their usual diet plus 1000 kcal per day from sucrose or fat, respectively. Group 3 had an isocaloric diet with a low-sucrose content, while group 4 ate low-fat high carbohydrate diets. Before change of diet the total group of volunteers showed an inverse correlation between insulin binding and average daily sucrose intake (R = − 0.52, p<0.01). An excessive sucrose consumption (group 1) was associated with a reduction both of insulin binding (p<0.05) and insulin sensitivity (p<0.05). The changes of the two variables were parallel (R = 0.95, p<0.05). An abundant fat intake (group 2) was also accompanied by a decrease of insulin binding (p<0.05). However, insulin sensitivity was unaltered (p>0.1). A rise of insulin binding (p<0.05) followed the isocaloric, low-sucrose diet (group 3) whereas the insulin sensitivity was unchanged (p>0.1). After the isocaloric, low-fat diet (group 4) no significant change of insulin binding occurred (p>0.1) whereas the insulin sensitivity increased (p<0.05). We conclude that diet and especially the dietary sucrose content affect insulin binding to human monocytes. Evidence is presented that changes of insulin sensitivity following hyperalimentation of sucrose may be induced through alterations of insulin receptors.
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Himsworth, H.P.: Dietetic factors determining the glucose tolerance and sensitivity to insulin of healthy man. Clin. Sci. Mol. Med.2, 67–94 (1935)
Wilkerson, H.L.C., Hyman, H., Kaufman, M., McCuistion, A.C., O'S Francis, J.: Diagnostic evaluation of oral glucose tolerance tests in nondiabetic subjects after various levels of carbohydrate intake. N. Engl. J. Med.262, 1047–1053 (1960)
Anderson, J.W., Herman, R.H.: Effect of fasting, caloric restriction, and refeeding on glucose tolerance of normal men. Am. J. Clin. Nutr.25, 41–52 (1972)
Sims, E.A.H., Danforth, E., Jr., Horton, E.S., Bray, G.A., Glennon, J.A., Salans, L.B.: Endocrine and metabolic effects of experimental obesity in man. Recent Prog. Horm. Res.29, 457–494 (1973)
Szanto, S., Yudkin, J.: The effect of dietary sucrose on blood lipids, serum insulin, platelet adhesiveness and body weight in human volunteers. Postgrad. Med. J.45, 602–607 (1969)
Olefsky, J.M.: The insulin receptor: Its role in insulin resistance of obesity and diabetes (Review). Diabetes25, 1154–1161 (1977)
Beck-Nielsen, H.: The pathogenic role of an insulin receptor defect in diabetes mellitus of obese. Diabetes (in press)
Harrison, L.C., Martin, F.I.R., Melick, R.A.: Insulin receptor binding in isolated fat cells and insulin sensitivity in obese. J. Clin. Invest.58, 1435–1441 (1976)
Beck-Nielsen, H., Pedersen, O.: Insulin receptors on monocytes of young healthy persons correlated to glucose tolerance and insulin sensitivity. Diabetologia14, 159–163 (1978)
Helms, P.: Fødevaretabeller. Copenhagen: Akademisk Forlag 1973
Heding, L.G.: A simplified insulin radioimmunoassay method. In: L. Donato et al. (Ed.): Labelled proteins in tracer studies 329–333. Brussels: Euratom 1966
Feteris, W.A.: A serum glucose method without protein precipitation. Am. J. Med. Technol.31, 17–21 (1965)
Pedersen, O., Beck-Nielsen, H.: A study of insulin receptors in human mononuclear leucocytes. Acta Endocrinol. (Kbh.)83, 556–564 (1976)
Jørgensen, K.H., Binder, C.:125I-insulin as a tracer of insulin in different chemical processes. In: L. Donato et al. (Ed.): Labelled proteins in tracer studies, 329–333. Brussels: Euratom 1966
Freychet, P., Roth, J., Neville, D.M. Jr.: Monoiodoinsulin: demonstration of its biological activity and binding to fat cells and liver membranes. Biochem. Biophys. Res. Commun.43, 400–408 (1966)
Beck-Nielsen, H., Pedersen, O., Kragballe, K., Sørensen, N.S.: The monocyte as a model for the study of insulin receptors in man. Diabetologia13, 563–569 (1977)
Scatchard, G.: The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci.51, 660–672 (1949)
Pollet, R.J., Standaert, M.L., Haase, B.A.: Insulin binding to the human lymphocyte receptor. J. Biol. Chem.252, 5828–5834 (1977)
Ip, C., Tepperman, H.M., Holohan, P., Tepperman, J.: Insulin binding and insulin response of adipocytes from rats adapted to fat feeding. J. Lipid Res.17, 588–599 (1976)
Cushman, S.W., Salans, L.B.: Effects of dietary composition on insulin binding in isolated rat adipocytes (Abstract). Clin. Res.23, 317 (1975)
Dahms, W.T., Atkinson, R.L., Bray, G.A.: Insulin resistance and the binding of insulin to adipocytes (Abstract). Clin. Res.24, 116 (1976)
Gavin, J.R., III, Roth, J., Neville, D.M. Jr., DeMeyts, P., Buell, D.N.: Insulin-dependent regulation of insulin receptor concentrations. A direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA71, 84–88 (1974)
Olefsky, J.M.: Effects of fasting on insulin binding, glucose transport, and glucose oxidation in isolated rat adipocytes: relationship between insulin binding and insulin action. J. Clin. Invest.58, 1450–1460 (1977)
Olefsky, J.M.: Decreased insulin binding to adipocytes and circulating monocytes from obese subjects. J. Clin. Invest.57, 1165–1172 (1976)
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Beck-Nielsen, H., Pedersen, O. & Schwartz Sørensen, N. Effects of diet on the cellular insulin binding and the insulin sensitivity in young healthy subjects. Diabetologia 15, 289–296 (1978). https://doi.org/10.1007/BF02573821
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DOI: https://doi.org/10.1007/BF02573821