Abstract
The enzymes pepsin, α-chymotrypsin, trypsin, RNase and DNase were applied to preparations of human metaphase chromosomes before staining to study whether dissociable materials related to the formation of G-, Q- and C-bands would be seen. Treatment with active pepsin but not the other enzymes revealed material with ribonucleo-protein properties which dissociated from the chromosomes and formed a halo. — Lateral extensions from the chromatids stretched to the rim of the halo and appeared at positions corresponding to G-bands. A G-band may be defined as a ring of stable chromatid-matrix binding at positions where the chromatids coil to form lateral extensions.
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Byarugaba, W. A pepsin-revealed material possibly related to chromosomal banding. Chromosoma 67, 275–283 (1978). https://doi.org/10.1007/BF02569040
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DOI: https://doi.org/10.1007/BF02569040