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Cellular affinity is the only deciding factor for microcarrier selection for the cultivation of anchorage dependent cells

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Biotechnology Techniques

Summary

Vero and BHK-21 cells are not attached and spreaded equally on the same microcarrier. BHK-21 cells require a microenvironment similar to the mammalian tissue for attachment and spreading. Cellular affinity, which control adhesion to the surface, varied with the growth phase, membrane integrity and the type of microcarrier.

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Authors and Affiliations

  1. National Institute of Immunology Saheed Jit Singh Marg, 110 067, New Delhi, India

    Asok Mukhopadhyay

  2. Biochemical Engineering Research Centre, Indian Institute of Technology, Kauz Khas, 110 016, New Delhi, India

    S. N. Mukhopadhyay & G. P. Talwar

Authors
  1. Asok Mukhopadhyay
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  2. S. N. Mukhopadhyay
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  3. G. P. Talwar
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Mukhopadhyay, A., Mukhopadhyay, S.N. & Talwar, G.P. Cellular affinity is the only deciding factor for microcarrier selection for the cultivation of anchorage dependent cells. Biotechnol Tech 7, 173–176 (1993). https://doi.org/10.1007/BF02566143

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  • DOI: https://doi.org/10.1007/BF02566143

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